The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment
Many Enterococcus faecalis strains display tolerance or resistance to many antibiotics, but genes that contribute to the resistance cannot be specified. The multiresistant E. faecalis V583, for which the complete genome sequence is available, survives and grows in media containing relatively high le...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2010/483048 |
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| author | Ågot Aakra Heidi Vebø Ulf Indahl Lars Snipen Øystein Gjerstad Merete Lunde Ingolf F. Nes |
| author_facet | Ågot Aakra Heidi Vebø Ulf Indahl Lars Snipen Øystein Gjerstad Merete Lunde Ingolf F. Nes |
| author_sort | Ågot Aakra |
| collection | DOAJ |
| description | Many Enterococcus faecalis strains display tolerance or resistance to many antibiotics, but genes that contribute to the resistance cannot be specified. The multiresistant E. faecalis V583, for which the complete genome sequence is available, survives and grows in media containing relatively high levels of chloramphenicol. No specific genes coding for chloramphenicol resistance has been recognized in V583. We used microarrays to identify genes and mechanisms behind the tolerance to chloramphenicol in V583, by comparison of cells treated with subinhibitory concentrations of chloramphenicol and untreated V583 cells. During a time course experiment, more than 600 genes were significantly differentially transcribed. Since chloramphenicol affects protein synthesis in bacteria, many genes involved in protein synthesis, for example, genes for ribosomal proteins, were induced. Genes involved in amino acid biosynthesis, for example, genes for tRNA synthetases and energy metabolism were downregulated, mainly. Among the upregulated genes were EF1732 and EF1733, which code for potential chloramphenicol transporters. Efflux of drug out of the cells may be one mechanism used by V583 to overcome the effect of chloramphenicol. |
| format | Article |
| id | doaj-art-ff6166f4affa447dbc00122a3d30a99b |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-ff6166f4affa447dbc00122a3d30a99b2025-08-20T03:25:42ZengWileyInternational Journal of Microbiology1687-918X1687-91982010-01-01201010.1155/2010/483048483048The Response of Enterococcus faecalis V583 to Chloramphenicol TreatmentÅgot Aakra0Heidi Vebø1Ulf Indahl2Lars Snipen3Øystein Gjerstad4Merete Lunde5Ingolf F. Nes6Department of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayDepartment of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayDepartment of Mathematical Sciences and Technology, The Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayDepartment of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayDepartment of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayDepartment of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayDepartment of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayMany Enterococcus faecalis strains display tolerance or resistance to many antibiotics, but genes that contribute to the resistance cannot be specified. The multiresistant E. faecalis V583, for which the complete genome sequence is available, survives and grows in media containing relatively high levels of chloramphenicol. No specific genes coding for chloramphenicol resistance has been recognized in V583. We used microarrays to identify genes and mechanisms behind the tolerance to chloramphenicol in V583, by comparison of cells treated with subinhibitory concentrations of chloramphenicol and untreated V583 cells. During a time course experiment, more than 600 genes were significantly differentially transcribed. Since chloramphenicol affects protein synthesis in bacteria, many genes involved in protein synthesis, for example, genes for ribosomal proteins, were induced. Genes involved in amino acid biosynthesis, for example, genes for tRNA synthetases and energy metabolism were downregulated, mainly. Among the upregulated genes were EF1732 and EF1733, which code for potential chloramphenicol transporters. Efflux of drug out of the cells may be one mechanism used by V583 to overcome the effect of chloramphenicol.http://dx.doi.org/10.1155/2010/483048 |
| spellingShingle | Ågot Aakra Heidi Vebø Ulf Indahl Lars Snipen Øystein Gjerstad Merete Lunde Ingolf F. Nes The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment International Journal of Microbiology |
| title | The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment |
| title_full | The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment |
| title_fullStr | The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment |
| title_full_unstemmed | The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment |
| title_short | The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment |
| title_sort | response of enterococcus faecalis v583 to chloramphenicol treatment |
| url | http://dx.doi.org/10.1155/2010/483048 |
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