Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient
The plating efficiency of neurons in culture is highly dependent on the concentration of cells used to establish the monolayer. A discontinuous iodixanol gradient permits both the production of a viable concentrated suspension of neurons and purification from other brain tissue elements. The gradien...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.850 |
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| _version_ | 1832551436473335808 |
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| author | John Graham |
| author_facet | John Graham |
| author_sort | John Graham |
| collection | DOAJ |
| description | The plating efficiency of neurons in culture is highly dependent on the concentration of cells used to establish the monolayer. A discontinuous iodixanol gradient permits both the production of a viable concentrated suspension of neurons and purification from other brain tissue elements. The gradient that is described in this Protocol Article is applicable to brain tissue from rat and also from human biopsy specimens. |
| format | Article |
| id | doaj-art-ff5bac28934f4f4abfa2b959ed537c3a |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ff5bac28934f4f4abfa2b959ed537c3a2025-02-03T06:01:29ZengWileyThe Scientific World Journal1537-744X2002-01-0121634163710.1100/tsw.2002.850Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density GradientJohn Graham0School of Biomolecular Sciences, Liverpool John Moores University, UKThe plating efficiency of neurons in culture is highly dependent on the concentration of cells used to establish the monolayer. A discontinuous iodixanol gradient permits both the production of a viable concentrated suspension of neurons and purification from other brain tissue elements. The gradient that is described in this Protocol Article is applicable to brain tissue from rat and also from human biopsy specimens.http://dx.doi.org/10.1100/tsw.2002.850 |
| spellingShingle | John Graham Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient The Scientific World Journal |
| title | Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient |
| title_full | Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient |
| title_fullStr | Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient |
| title_full_unstemmed | Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient |
| title_short | Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient |
| title_sort | isolation of rat and human hippocampal neuron fractions in a discontinuous density gradient |
| url | http://dx.doi.org/10.1100/tsw.2002.850 |
| work_keys_str_mv | AT johngraham isolationofratandhumanhippocampalneuronfractionsinadiscontinuousdensitygradient |