Three-dimensional single-cell transcriptome imaging of thick tissues
Multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows genome-scale imaging of RNAs in individual cells in intact tissues. To date, MERFISH has been applied to image thin-tissue samples of ~10 µm thickness. Here, we present a thick-tissue three-dimensional (3D) MERFISH imaging...
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Main Authors: | Rongxin Fang, Aaron Halpern, Mohammed Mostafizur Rahman, Zhengkai Huang, Zhiyun Lei, Sebastian J Hell, Catherine Dulac, Xiaowei Zhuang |
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Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2024-12-01
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Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/90029 |
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