AAV-mediated transduction of songbird retina
IntroductionGenetic manipulation of murine retinal tissue through ocular administration of adeno-associated viruses (AAVs) has become a standard technique to investigate a multitude of mechanisms underlying retinal physiology. Resultantly, developments of recombinant viral vectors with improved tran...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2025.1549585/full |
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| author | Pranav Kumar Seth Pranav Kumar Seth Dominik Heyers Dominik Heyers Baladev Satish Ezequiel Mendoza Katrin Haase Lisa Borowsky Isabelle Musielak Karl-Wilhelm Koch Karl-Wilhelm Koch Regina Feederle Constance Scharff Karin Dedek Karin Dedek Henrik Mouritsen Henrik Mouritsen |
| author_facet | Pranav Kumar Seth Pranav Kumar Seth Dominik Heyers Dominik Heyers Baladev Satish Ezequiel Mendoza Katrin Haase Lisa Borowsky Isabelle Musielak Karl-Wilhelm Koch Karl-Wilhelm Koch Regina Feederle Constance Scharff Karin Dedek Karin Dedek Henrik Mouritsen Henrik Mouritsen |
| author_sort | Pranav Kumar Seth |
| collection | DOAJ |
| description | IntroductionGenetic manipulation of murine retinal tissue through ocular administration of adeno-associated viruses (AAVs) has become a standard technique to investigate a multitude of mechanisms underlying retinal physiology. Resultantly, developments of recombinant viral vectors with improved transduction efficiency and further methodological improvements have mostly focused on murine tissue, whereas AAVs successfully targeting avian retinae have remained scarce.MethodologyUsing a custom-designed injection setup, we identified a viral serotype with the capability to successfully induce widespread transduction of the bird retina.ResultsIntravitreal administration of an AAV type 2/9 encoding for enhanced green fluorescent protein (EGFP) in night-migratory European robins (Erithacus rubecula) resulted in transduction coverages of up to 60% within retinal tissue. Subsequent immunohistochemical analyses revealed that the AAV2/9-EGFP serotype almost exclusively targeted photoreceptors: rods, various single cones (UV, blue, green, and red cones), and both (accessory and principal) members of double cones.DiscussionThe consistently high and photoreceptor-specific transduction efficiency makes the AAV2/9 serotype a powerful tool for carrying out genetic manipulations in avian retinal photoreceptors, thus opening a wealth of opportunities to investigate physiological aspects underlying retinal processing in birds, such as physiological recordings and/or post-transductional behavioural readouts for future vision-related research. |
| format | Article |
| id | doaj-art-ff4b5d56b4c1498cb2c3ef34e67b4742 |
| institution | DOAJ |
| issn | 1664-042X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj-art-ff4b5d56b4c1498cb2c3ef34e67b47422025-08-20T03:02:56ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2025-03-011610.3389/fphys.2025.15495851549585AAV-mediated transduction of songbird retinaPranav Kumar Seth0Pranav Kumar Seth1Dominik Heyers2Dominik Heyers3Baladev Satish4Ezequiel Mendoza5Katrin Haase6Lisa Borowsky7Isabelle Musielak8Karl-Wilhelm Koch9Karl-Wilhelm Koch10Regina Feederle11Constance Scharff12Karin Dedek13Karin Dedek14Henrik Mouritsen15Henrik Mouritsen16Neurosensorics Group/Animal Navigation, Institute of Biology and Environmental Sciences, Carl on Ossietzky University of Oldenburg, Oldenburg, GermanySussex Neuroscience, School of Life Sciences, University of Sussex, Brighton, United KingdomNeurosensorics Group/Animal Navigation, Institute of Biology and Environmental Sciences, Carl on Ossietzky University of Oldenburg, Oldenburg, GermanyResearch Centre for Neurosensory Science, Carl von Ossietzky University of Oldenburg, Oldenburg, GermanyNeurosensorics Group/Animal Navigation, Institute of Biology and Environmental Sciences, Carl on Ossietzky University of Oldenburg, Oldenburg, GermanyInstitut für Biologie, Freie Universität Berlin, Berlin, GermanyNeurosensorics Group/Animal Navigation, Institute of Biology and Environmental Sciences, Carl on Ossietzky University of Oldenburg, Oldenburg, GermanyNeurosensorics Group/Animal Navigation, Institute of Biology and Environmental Sciences, Carl on Ossietzky University of Oldenburg, Oldenburg, GermanyNeurosensorics Group/Animal Navigation, Institute of Biology and Environmental Sciences, Carl on Ossietzky University of Oldenburg, Oldenburg, GermanyResearch Centre for Neurosensory Science, Carl von Ossietzky University of Oldenburg, Oldenburg, GermanyDepartment of Neuroscience, Biochemistry Group, University of Oldenburg, Oldenburg, GermanyMonoclonal Antibody Core Facility, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, GermanyInstitut für Biologie, Freie Universität Berlin, Berlin, GermanyNeurosensorics Group/Animal Navigation, Institute of Biology and Environmental Sciences, Carl on Ossietzky University of Oldenburg, Oldenburg, GermanyResearch Centre for Neurosensory Science, Carl von Ossietzky University of Oldenburg, Oldenburg, GermanyNeurosensorics Group/Animal Navigation, Institute of Biology and Environmental Sciences, Carl on Ossietzky University of Oldenburg, Oldenburg, GermanyResearch Centre for Neurosensory Science, Carl von Ossietzky University of Oldenburg, Oldenburg, GermanyIntroductionGenetic manipulation of murine retinal tissue through ocular administration of adeno-associated viruses (AAVs) has become a standard technique to investigate a multitude of mechanisms underlying retinal physiology. Resultantly, developments of recombinant viral vectors with improved transduction efficiency and further methodological improvements have mostly focused on murine tissue, whereas AAVs successfully targeting avian retinae have remained scarce.MethodologyUsing a custom-designed injection setup, we identified a viral serotype with the capability to successfully induce widespread transduction of the bird retina.ResultsIntravitreal administration of an AAV type 2/9 encoding for enhanced green fluorescent protein (EGFP) in night-migratory European robins (Erithacus rubecula) resulted in transduction coverages of up to 60% within retinal tissue. Subsequent immunohistochemical analyses revealed that the AAV2/9-EGFP serotype almost exclusively targeted photoreceptors: rods, various single cones (UV, blue, green, and red cones), and both (accessory and principal) members of double cones.DiscussionThe consistently high and photoreceptor-specific transduction efficiency makes the AAV2/9 serotype a powerful tool for carrying out genetic manipulations in avian retinal photoreceptors, thus opening a wealth of opportunities to investigate physiological aspects underlying retinal processing in birds, such as physiological recordings and/or post-transductional behavioural readouts for future vision-related research.https://www.frontiersin.org/articles/10.3389/fphys.2025.1549585/fullAAVavian retinaphotoreceptorsintravitreal injectionEuropean robinopsin |
| spellingShingle | Pranav Kumar Seth Pranav Kumar Seth Dominik Heyers Dominik Heyers Baladev Satish Ezequiel Mendoza Katrin Haase Lisa Borowsky Isabelle Musielak Karl-Wilhelm Koch Karl-Wilhelm Koch Regina Feederle Constance Scharff Karin Dedek Karin Dedek Henrik Mouritsen Henrik Mouritsen AAV-mediated transduction of songbird retina Frontiers in Physiology AAV avian retina photoreceptors intravitreal injection European robin opsin |
| title | AAV-mediated transduction of songbird retina |
| title_full | AAV-mediated transduction of songbird retina |
| title_fullStr | AAV-mediated transduction of songbird retina |
| title_full_unstemmed | AAV-mediated transduction of songbird retina |
| title_short | AAV-mediated transduction of songbird retina |
| title_sort | aav mediated transduction of songbird retina |
| topic | AAV avian retina photoreceptors intravitreal injection European robin opsin |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2025.1549585/full |
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