Automated DNA Sequencing Requiring No DNA Template Purification

A critical component in automated fluorescent DNA sequencing is good quality of DNA template. In an effort to reduce the dependence of sequencing success on DNA template quality, the effect of heat-soaked PCR on automated sequencing reactions has been examined. We have found that the heat-soaked PCR...

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Bibliographic Details
Main Authors: Qianfa Chen, Catherine Neville, Alex MacKenzie, Robert G. Korneluk
Format: Article
Language:English
Published: Taylor & Francis Group 1996-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/96213st02
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