Immunomodulatory effects of type II arabinogalactans fraction from Anoectochilus formosanus in a murine model of OVA-induced airway hyperresponsiveness
Polysaccharides have been investigated for their potential role in modulating allergic asthma, a chronic respiratory disease characterized by airway hyperresponsiveness (AHR). This study aimed to evaluate the immunomodulatory effects of a type II arabinogalactan fraction from Anoectochilus formosanu...
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001859 |
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| author | Yi-Lun Huang Wen-Chuan Lin Vipul Wayal Chang-Chi Hsieh |
| author_facet | Yi-Lun Huang Wen-Chuan Lin Vipul Wayal Chang-Chi Hsieh |
| author_sort | Yi-Lun Huang |
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| description | Polysaccharides have been investigated for their potential role in modulating allergic asthma, a chronic respiratory disease characterized by airway hyperresponsiveness (AHR). This study aimed to evaluate the immunomodulatory effects of a type II arabinogalactan fraction from Anoectochilus formosanus (AGAF) on allergic responses in a murine model of ovalbumin (OVA)-induced allergic asthma. BALB/c mice were treated with various doses of AGAF (350, 700, and 1750 mg/kg), while the control groups received either distilled water (negative control) or prednisolone (10 mg/kg, positive control) over a six-week preventive regimen. After this period, mice were challenged with OVA to examine the immunomodulatory effects of AGAF. We investigated cellular differentiation and proliferation in lymphoid organs, anti-ovalbumin immunoglobulin levels, bronchial hyperresponsiveness, lung inflammation, histopathological changes and immunohistochemical analysis of TSLP, JNK and TGF-β1 in lungs. Six weeks of AGAF administration significantly downregulated thymic stromal lymphopoietin (TSLP) and c-Jun N-terminal kinase (JNK) expressions in the airways, reduced airway inflammatory cell infiltration, inhibited mast cell degranulation, decreased mucus production, modulated T helper (Th)1/Th2 differentiation, lowered anti-OVA IgE levels, and alleviated bronchoconstriction. These findings suggest that AGAF can influence T and B cell development and modulate allergic responses, making it a potential therapeutic agent for allergic asthma. |
| format | Article |
| id | doaj-art-ff3ec89d435747ad87e9aa9d73ab8e4e |
| institution | OA Journals |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-ff3ec89d435747ad87e9aa9d73ab8e4e2025-08-20T02:06:32ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010084710.1016/j.carpta.2025.100847Immunomodulatory effects of type II arabinogalactans fraction from Anoectochilus formosanus in a murine model of OVA-induced airway hyperresponsivenessYi-Lun Huang0Wen-Chuan Lin1Vipul Wayal2Chang-Chi Hsieh3Department of Animal Science and Biotechnology, Tunghai University, Taichung 407224, TaiwanDepartment of Pharmacology, School of Medicine, China Medical University, Taichung 40402, TaiwanDepartment of Animal Science and Biotechnology, Tunghai University, Taichung 407224, Taiwan; Corresponding authors at: Department of Animal Science and Biotechnology, Tunghai University, Taichung 407224, Taiwan.Department of Animal Science and Biotechnology, Tunghai University, Taichung 407224, Taiwan; Corresponding authors at: Department of Animal Science and Biotechnology, Tunghai University, Taichung 407224, Taiwan.Polysaccharides have been investigated for their potential role in modulating allergic asthma, a chronic respiratory disease characterized by airway hyperresponsiveness (AHR). This study aimed to evaluate the immunomodulatory effects of a type II arabinogalactan fraction from Anoectochilus formosanus (AGAF) on allergic responses in a murine model of ovalbumin (OVA)-induced allergic asthma. BALB/c mice were treated with various doses of AGAF (350, 700, and 1750 mg/kg), while the control groups received either distilled water (negative control) or prednisolone (10 mg/kg, positive control) over a six-week preventive regimen. After this period, mice were challenged with OVA to examine the immunomodulatory effects of AGAF. We investigated cellular differentiation and proliferation in lymphoid organs, anti-ovalbumin immunoglobulin levels, bronchial hyperresponsiveness, lung inflammation, histopathological changes and immunohistochemical analysis of TSLP, JNK and TGF-β1 in lungs. Six weeks of AGAF administration significantly downregulated thymic stromal lymphopoietin (TSLP) and c-Jun N-terminal kinase (JNK) expressions in the airways, reduced airway inflammatory cell infiltration, inhibited mast cell degranulation, decreased mucus production, modulated T helper (Th)1/Th2 differentiation, lowered anti-OVA IgE levels, and alleviated bronchoconstriction. These findings suggest that AGAF can influence T and B cell development and modulate allergic responses, making it a potential therapeutic agent for allergic asthma.http://www.sciencedirect.com/science/article/pii/S2666893925001859Allergic asthmaAirway hyperresponsivenessAnoectochilus formosanusArabinogalactanTSLPJNK |
| spellingShingle | Yi-Lun Huang Wen-Chuan Lin Vipul Wayal Chang-Chi Hsieh Immunomodulatory effects of type II arabinogalactans fraction from Anoectochilus formosanus in a murine model of OVA-induced airway hyperresponsiveness Carbohydrate Polymer Technologies and Applications Allergic asthma Airway hyperresponsiveness Anoectochilus formosanus Arabinogalactan TSLP JNK |
| title | Immunomodulatory effects of type II arabinogalactans fraction from Anoectochilus formosanus in a murine model of OVA-induced airway hyperresponsiveness |
| title_full | Immunomodulatory effects of type II arabinogalactans fraction from Anoectochilus formosanus in a murine model of OVA-induced airway hyperresponsiveness |
| title_fullStr | Immunomodulatory effects of type II arabinogalactans fraction from Anoectochilus formosanus in a murine model of OVA-induced airway hyperresponsiveness |
| title_full_unstemmed | Immunomodulatory effects of type II arabinogalactans fraction from Anoectochilus formosanus in a murine model of OVA-induced airway hyperresponsiveness |
| title_short | Immunomodulatory effects of type II arabinogalactans fraction from Anoectochilus formosanus in a murine model of OVA-induced airway hyperresponsiveness |
| title_sort | immunomodulatory effects of type ii arabinogalactans fraction from anoectochilus formosanus in a murine model of ova induced airway hyperresponsiveness |
| topic | Allergic asthma Airway hyperresponsiveness Anoectochilus formosanus Arabinogalactan TSLP JNK |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925001859 |
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