Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study
The reaction of anti-sperm antibody-positive sera from infertile women with fractionated mouse ovarian antigens was measured by enzyme-linked immunosorbent assay (ELISA). Antigens were obtained by extraction for nuclear matrix and intermediate filaments (NM-IF) producing three protein fractions – so...
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| Format: | Article |
| Language: | English |
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Medical University - Pleven
2017-11-01
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| Series: | Journal of Biomedical & Clinical Research |
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| Online Access: | https://jbcr.arphahub.com/article/34455/download/pdf/ |
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| author | Stefka Delimitreva Ralitsa Zhivkova Irina Chakarova Valentina Hadzhinesheva Vladislav Lazarov Dimitrina Dimitrova-Dikanarova |
| author_facet | Stefka Delimitreva Ralitsa Zhivkova Irina Chakarova Valentina Hadzhinesheva Vladislav Lazarov Dimitrina Dimitrova-Dikanarova |
| author_sort | Stefka Delimitreva |
| collection | DOAJ |
| description | The reaction of anti-sperm antibody-positive sera from infertile women with fractionated mouse ovarian antigens was measured by enzyme-linked immunosorbent assay (ELISA). Antigens were obtained by extraction for nuclear matrix and intermediate filaments (NM-IF) producing three protein fractions – soluble, cytoskeletal and NM-IF. The results showed that sera from some infertile patients, but not control sera, react with either the soluble fraction or the NM-IF fraction. The reaction with soluble proteins was most likely directed against surface antigens, possibly aggravating the fertility problems, while the anti-NM-IF antibodies could indicate release of insoluble intracellular components by tissue damage of unknown origin. |
| format | Article |
| id | doaj-art-ff3b2b13556e4b49986e59d8977207de |
| institution | DOAJ |
| issn | 1313-9053 |
| language | English |
| publishDate | 2017-11-01 |
| publisher | Medical University - Pleven |
| record_format | Article |
| series | Journal of Biomedical & Clinical Research |
| spelling | doaj-art-ff3b2b13556e4b49986e59d8977207de2025-08-20T02:48:09ZengMedical University - PlevenJournal of Biomedical & Clinical Research1313-90532017-11-01101373910.1515/jbcr-2017-000734455Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot StudyStefka DelimitrevaRalitsa ZhivkovaIrina ChakarovaValentina HadzhineshevaVladislav LazarovDimitrina Dimitrova-DikanarovaThe reaction of anti-sperm antibody-positive sera from infertile women with fractionated mouse ovarian antigens was measured by enzyme-linked immunosorbent assay (ELISA). Antigens were obtained by extraction for nuclear matrix and intermediate filaments (NM-IF) producing three protein fractions – soluble, cytoskeletal and NM-IF. The results showed that sera from some infertile patients, but not control sera, react with either the soluble fraction or the NM-IF fraction. The reaction with soluble proteins was most likely directed against surface antigens, possibly aggravating the fertility problems, while the anti-NM-IF antibodies could indicate release of insoluble intracellular components by tissue damage of unknown origin.https://jbcr.arphahub.com/article/34455/download/pdf/nuclear matrixintermediate filamentsanti-ovari |
| spellingShingle | Stefka Delimitreva Ralitsa Zhivkova Irina Chakarova Valentina Hadzhinesheva Vladislav Lazarov Dimitrina Dimitrova-Dikanarova Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study Journal of Biomedical & Clinical Research nuclear matrix intermediate filaments anti-ovari |
| title | Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study |
| title_full | Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study |
| title_fullStr | Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study |
| title_full_unstemmed | Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study |
| title_short | Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study |
| title_sort | reaction of sera from infertile female patients with fractionated phylogenetically conserved ovarian antigens measured by elisa a pilot study |
| topic | nuclear matrix intermediate filaments anti-ovari |
| url | https://jbcr.arphahub.com/article/34455/download/pdf/ |
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