Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study
The reaction of anti-sperm antibody-positive sera from infertile women with fractionated mouse ovarian antigens was measured by enzyme-linked immunosorbent assay (ELISA). Antigens were obtained by extraction for nuclear matrix and intermediate filaments (NM-IF) producing three protein fractions – so...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Medical University - Pleven
2017-11-01
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| Series: | Journal of Biomedical & Clinical Research |
| Subjects: | |
| Online Access: | https://jbcr.arphahub.com/article/34455/download/pdf/ |
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| Summary: | The reaction of anti-sperm antibody-positive sera from infertile women with fractionated mouse ovarian antigens was measured by enzyme-linked immunosorbent assay (ELISA). Antigens were obtained by extraction for nuclear matrix and intermediate filaments (NM-IF) producing three protein fractions – soluble, cytoskeletal and NM-IF. The results showed that sera from some infertile patients, but not control sera, react with either the soluble fraction or the NM-IF fraction. The reaction with soluble proteins was most likely directed against surface antigens, possibly aggravating the fertility problems, while the anti-NM-IF antibodies could indicate release of insoluble intracellular components by tissue damage of unknown origin. |
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| ISSN: | 1313-9053 |