Therapeutic Effects of PSL-Loaded PLGA-PEG-PLGA NPs in Allergic Contact Dermatitis Model Mice
This study focused on the poly(DL-lactide-<i>co</i>-glycolide)-<i>block</i>-poly(ethylene glycol)-<i>block</i>-poly(DL-lactide-<i>co</i>-glycolide) (PLGA-PEG-PLGA) triblock copolymer, which was recently reported as a novel material for polymeric nanopa...
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MDPI AG
2025-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/30/15/3292 |
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| author | Ryo Fujisawa Ryuse Sakurai Takeshi Oshizaka Kenji Mori Akiyoshi Saitoh Issei Takeuchi Kenji Sugibayashi |
| author_facet | Ryo Fujisawa Ryuse Sakurai Takeshi Oshizaka Kenji Mori Akiyoshi Saitoh Issei Takeuchi Kenji Sugibayashi |
| author_sort | Ryo Fujisawa |
| collection | DOAJ |
| description | This study focused on the poly(DL-lactide-<i>co</i>-glycolide)-<i>block</i>-poly(ethylene glycol)-<i>block</i>-poly(DL-lactide-<i>co</i>-glycolide) (PLGA-PEG-PLGA) triblock copolymer, which was recently reported as a novel material for polymeric nanoparticles to replace poly(DL-lactide-<i>co</i>-glycolide) (PLGA) as a drug carrier for prednisolone (PSL), and aimed to evaluate the efficacy of PSL-loaded PLGA-PEG-PLGA nanoparticles (NPs) against allergic contact dermatitis (ACD). PSL-loaded PLGA-PEG-PLGA NPs were prepared using the nanoprecipitation method, and their particle size distribution and mean particle size were measured using dynamic light scattering. 1-Fluoro-2,4-dinitrobenzene (DNFB) was used to create a mouse model of contact hypersensitivity (CHS). PSL-loaded PLGA-PEG-PLGA NPs were administered before sensitization with DNFB, and the therapeutic effect was evaluated by quantifying intracutaneous TNF-α and IL-4 levels suing ELISA. When PSL-loaded PLGA-PEG-PLGA NPs were administered before sensitization, TNF-α expression and IL-4 statements were significantly lower in the PSL-loaded PLGA-PEG-PLGA NP group than in the non-treated group. No significant difference was observed between the PSL-loaded PLGA-PEG-PLGA NP and PSL-loaded ointment groups, even though the steroid dose was 40 times lower than in the PSL-containing ointment. These results suggest that PSL-loaded PLGA-PEG-PLGA NPs may have a better effect in the treatment of ACD than PSL-loaded PLGA NPs. |
| format | Article |
| id | doaj-art-ff3af476833e4d6f90f1b42f24210f7a |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-ff3af476833e4d6f90f1b42f24210f7a2025-08-20T03:04:43ZengMDPI AGMolecules1420-30492025-08-013015329210.3390/molecules30153292Therapeutic Effects of PSL-Loaded PLGA-PEG-PLGA NPs in Allergic Contact Dermatitis Model MiceRyo Fujisawa0Ryuse Sakurai1Takeshi Oshizaka2Kenji Mori3Akiyoshi Saitoh4Issei Takeuchi5Kenji Sugibayashi6Department of Medical Pharmacy, Graduate School of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, JapanFaculty of Pharmaceutical Sciences, Tokyo University of Science, 2641, Yamazaki, Noda 278-8510, JapanDepartment of Medical Pharmacy, Graduate School of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, JapanDepartment of Medical Pharmacy, Graduate School of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, JapanFaculty of Pharmaceutical Sciences, Tokyo University of Science, 2641, Yamazaki, Noda 278-8510, JapanDepartment of Medical Pharmacy, Graduate School of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, JapanDepartment of Medical Pharmacy, Graduate School of Pharmaceutical Sciences, Josai International University, 1 Gumyo Togane, Chiba 283-0002, JapanThis study focused on the poly(DL-lactide-<i>co</i>-glycolide)-<i>block</i>-poly(ethylene glycol)-<i>block</i>-poly(DL-lactide-<i>co</i>-glycolide) (PLGA-PEG-PLGA) triblock copolymer, which was recently reported as a novel material for polymeric nanoparticles to replace poly(DL-lactide-<i>co</i>-glycolide) (PLGA) as a drug carrier for prednisolone (PSL), and aimed to evaluate the efficacy of PSL-loaded PLGA-PEG-PLGA nanoparticles (NPs) against allergic contact dermatitis (ACD). PSL-loaded PLGA-PEG-PLGA NPs were prepared using the nanoprecipitation method, and their particle size distribution and mean particle size were measured using dynamic light scattering. 1-Fluoro-2,4-dinitrobenzene (DNFB) was used to create a mouse model of contact hypersensitivity (CHS). PSL-loaded PLGA-PEG-PLGA NPs were administered before sensitization with DNFB, and the therapeutic effect was evaluated by quantifying intracutaneous TNF-α and IL-4 levels suing ELISA. When PSL-loaded PLGA-PEG-PLGA NPs were administered before sensitization, TNF-α expression and IL-4 statements were significantly lower in the PSL-loaded PLGA-PEG-PLGA NP group than in the non-treated group. No significant difference was observed between the PSL-loaded PLGA-PEG-PLGA NP and PSL-loaded ointment groups, even though the steroid dose was 40 times lower than in the PSL-containing ointment. These results suggest that PSL-loaded PLGA-PEG-PLGA NPs may have a better effect in the treatment of ACD than PSL-loaded PLGA NPs.https://www.mdpi.com/1420-3049/30/15/3292poly(DL-lactide-<i>co</i>-glycolide)-<i>block</i>-poly(ethylene glycol)-<i>block</i>-poly(DL-lactide-<i>co</i>-glycolide) (PLGA-PEG-PLGA)nanoparticleallergic contact dermatitisprednisolone |
| spellingShingle | Ryo Fujisawa Ryuse Sakurai Takeshi Oshizaka Kenji Mori Akiyoshi Saitoh Issei Takeuchi Kenji Sugibayashi Therapeutic Effects of PSL-Loaded PLGA-PEG-PLGA NPs in Allergic Contact Dermatitis Model Mice Molecules poly(DL-lactide-<i>co</i>-glycolide)-<i>block</i>-poly(ethylene glycol)-<i>block</i>-poly(DL-lactide-<i>co</i>-glycolide) (PLGA-PEG-PLGA) nanoparticle allergic contact dermatitis prednisolone |
| title | Therapeutic Effects of PSL-Loaded PLGA-PEG-PLGA NPs in Allergic Contact Dermatitis Model Mice |
| title_full | Therapeutic Effects of PSL-Loaded PLGA-PEG-PLGA NPs in Allergic Contact Dermatitis Model Mice |
| title_fullStr | Therapeutic Effects of PSL-Loaded PLGA-PEG-PLGA NPs in Allergic Contact Dermatitis Model Mice |
| title_full_unstemmed | Therapeutic Effects of PSL-Loaded PLGA-PEG-PLGA NPs in Allergic Contact Dermatitis Model Mice |
| title_short | Therapeutic Effects of PSL-Loaded PLGA-PEG-PLGA NPs in Allergic Contact Dermatitis Model Mice |
| title_sort | therapeutic effects of psl loaded plga peg plga nps in allergic contact dermatitis model mice |
| topic | poly(DL-lactide-<i>co</i>-glycolide)-<i>block</i>-poly(ethylene glycol)-<i>block</i>-poly(DL-lactide-<i>co</i>-glycolide) (PLGA-PEG-PLGA) nanoparticle allergic contact dermatitis prednisolone |
| url | https://www.mdpi.com/1420-3049/30/15/3292 |
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