Expression of Dlx-5 and HLX Proteins in Odontogenic Cysts

Odontogenic cysts, commonly detected during routine examinations involving head and neck imaging such as orthopantomograms and computed tomography (CT), are classified into two groups: developmental and inflammatory. Radicular cysts, which belong to the inflammatory group, originate from odontogenic...

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Main Authors: Sinan Ateş, Uğur Topaloğlu, Mehmet Erdem Akbalik, Şeyma Keleş Karagözoğlu
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Life
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Online Access:https://www.mdpi.com/2075-1729/15/2/301
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author Sinan Ateş
Uğur Topaloğlu
Mehmet Erdem Akbalik
Şeyma Keleş Karagözoğlu
author_facet Sinan Ateş
Uğur Topaloğlu
Mehmet Erdem Akbalik
Şeyma Keleş Karagözoğlu
author_sort Sinan Ateş
collection DOAJ
description Odontogenic cysts, commonly detected during routine examinations involving head and neck imaging such as orthopantomograms and computed tomography (CT), are classified into two groups: developmental and inflammatory. Radicular cysts, which belong to the inflammatory group, originate from odontogenic epithelium, while dentigerous cysts of developmental origin are observed as a result of peri-coronal expansion of fluid in the dental follicle. The diagnosis and identification of odontogenic cysts rely on clinical, radiographic, and histological evaluations. This study aimed to demonstrate the expression of Dlx-5 and HLX proteins in radicular and dentigerous cysts. A total of 40 radicular and 40 dentigerous cysts were obtained from patients who visited private oral and dental health clinics in Bingöl and Diyarbakır provinces. After undergoing routine histological procedures, the cysts were stained using Masson’s Trichrome and immunohistochemistry techniques. As a result, the epithelium of radicular cysts was found to be keratinized stratified squamous, with hyaline (Rushton) bodies located within the epithelium. Dentigerous cysts, on the other hand, consisted of non-keratinized stratified squamous epithelium, rete ridges with hyperplastic areas, and inflammatory cell infiltrations. The immunoreactivity induced by Dlx-5 in epithelial and connective tissue cells of radicular and dentigerous cysts was found to be stronger than that of HLX. The positive expression of Dlx-5 and HLX proteins in radicular and dentigerous cysts suggests that these proteins may play a potential role in the pathogenesis of these cysts. Furthermore, it was considered that the expression of Dlx-5 and HLX might help reveal the behavioral differences between odontogenic cysts.
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spelling doaj-art-ff39c49a6154495da128cda7f8b0951c2025-08-20T02:44:50ZengMDPI AGLife2075-17292025-02-0115230110.3390/life15020301Expression of Dlx-5 and HLX Proteins in Odontogenic CystsSinan Ateş0Uğur Topaloğlu1Mehmet Erdem Akbalik2Şeyma Keleş Karagözoğlu3Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Bingöl, 12000 Bingöl, TürkiyeDepartment of Histology and Embryology, Faculty of Veterinary Medicine, Dicle University, 21280 Diyarbakır, TürkiyeDepartment of Histology and Embryology, Faculty of Veterinary Medicine, Dicle University, 21280 Diyarbakır, TürkiyeHealth Services Vocational School, University of Bingöl, 12000 Bingöl, TürkiyeOdontogenic cysts, commonly detected during routine examinations involving head and neck imaging such as orthopantomograms and computed tomography (CT), are classified into two groups: developmental and inflammatory. Radicular cysts, which belong to the inflammatory group, originate from odontogenic epithelium, while dentigerous cysts of developmental origin are observed as a result of peri-coronal expansion of fluid in the dental follicle. The diagnosis and identification of odontogenic cysts rely on clinical, radiographic, and histological evaluations. This study aimed to demonstrate the expression of Dlx-5 and HLX proteins in radicular and dentigerous cysts. A total of 40 radicular and 40 dentigerous cysts were obtained from patients who visited private oral and dental health clinics in Bingöl and Diyarbakır provinces. After undergoing routine histological procedures, the cysts were stained using Masson’s Trichrome and immunohistochemistry techniques. As a result, the epithelium of radicular cysts was found to be keratinized stratified squamous, with hyaline (Rushton) bodies located within the epithelium. Dentigerous cysts, on the other hand, consisted of non-keratinized stratified squamous epithelium, rete ridges with hyperplastic areas, and inflammatory cell infiltrations. The immunoreactivity induced by Dlx-5 in epithelial and connective tissue cells of radicular and dentigerous cysts was found to be stronger than that of HLX. The positive expression of Dlx-5 and HLX proteins in radicular and dentigerous cysts suggests that these proteins may play a potential role in the pathogenesis of these cysts. Furthermore, it was considered that the expression of Dlx-5 and HLX might help reveal the behavioral differences between odontogenic cysts.https://www.mdpi.com/2075-1729/15/2/301Dlx-5HLXradicular cystodontogenic cystimmunohistochemistry
spellingShingle Sinan Ateş
Uğur Topaloğlu
Mehmet Erdem Akbalik
Şeyma Keleş Karagözoğlu
Expression of Dlx-5 and HLX Proteins in Odontogenic Cysts
Life
Dlx-5
HLX
radicular cyst
odontogenic cyst
immunohistochemistry
title Expression of Dlx-5 and HLX Proteins in Odontogenic Cysts
title_full Expression of Dlx-5 and HLX Proteins in Odontogenic Cysts
title_fullStr Expression of Dlx-5 and HLX Proteins in Odontogenic Cysts
title_full_unstemmed Expression of Dlx-5 and HLX Proteins in Odontogenic Cysts
title_short Expression of Dlx-5 and HLX Proteins in Odontogenic Cysts
title_sort expression of dlx 5 and hlx proteins in odontogenic cysts
topic Dlx-5
HLX
radicular cyst
odontogenic cyst
immunohistochemistry
url https://www.mdpi.com/2075-1729/15/2/301
work_keys_str_mv AT sinanates expressionofdlx5andhlxproteinsinodontogeniccysts
AT ugurtopaloglu expressionofdlx5andhlxproteinsinodontogeniccysts
AT mehmeterdemakbalik expressionofdlx5andhlxproteinsinodontogeniccysts
AT seymakeleskaragozoglu expressionofdlx5andhlxproteinsinodontogeniccysts