(1→3)-α-<span style="font-variant: small-caps">d</span>-Glucan from the Pink Oyster Mushroom (<i>Pleurotus djamor</i>): Structural Features
(1→3)-α-<span style="font-variant: small-caps;">d</span>-Glucan is an important component of the cell wall of most fungi. The polymer has many applications, including as a therapeutic agent in the prevention or treatment of various diseases, as well as a heavy metal sorbent and...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/14/7/1272 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | (1→3)-α-<span style="font-variant: small-caps;">d</span>-Glucan is an important component of the cell wall of most fungi. The polymer has many applications, including as a therapeutic agent in the prevention or treatment of various diseases, as well as a heavy metal sorbent and a component of new materials used in the plastics industry. The presence of (1→3)-α-<span style="font-variant: small-caps;">d</span>-glucan (water-insoluble, alkali-soluble polysaccharide) in the cell wall of <i>Pleurotus djamor</i> (pink oyster mushroom) was confirmed using specific fluorophore-labeled antibodies. Therefore, the water-insoluble fraction (WI-ASF) of <i>P. djamor</i> B123 fruiting bodies was isolated by alkaline extraction and used for further analyses. The structural features of the WI-ASF were determined by composition analysis, linkage analysis, Fourier transform infrared and Raman spectroscopy, <sup>1</sup>H and <sup>13</sup>C nuclear magnetic resonance spectroscopy, scanning electron microscopy, as well as viscosity, specific rotation, and gel permeation chromatography. These studies revealed the presence of glucose units linked by α-glycosidic bonds and scanty amounts of mannose and xylose. Furthermore, methylation analysis of WI-ASF demonstrated that the (1→3)-linked glucopyranose (Glc<i>p</i>) is the primary moiety (86.4%) of the polymer, while the 3,4- and 3,6-substituted hexoses are the branching residues of the glucan. The results of chemical and spectroscopic investigations indicated that the analyzed WI-ASF is a (1→3)-linked α-<span style="font-variant: small-caps;">d</span>-glucan type with a molecular weight of 552 kDa. |
|---|---|
| ISSN: | 2304-8158 |