Antiviral Activity of Rambutan Peel Polyphenols Obtained Using Green Extraction Technology and Solvents

Rambutan peel is a great source of bioactive compounds, the same that, over the years, has been extracted using conventional technologies which have been proven to be harmful to the environment and potentially toxic to human beings. This study aimed to extract the same compounds using a hybridizatio...

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Main Authors: Christian Hernández-Hernández, Luis E. Estrada-Gil, Sonia A. Lozano-Sepúlveda, Ana M. Rivas-Estilla, Mayela Govea-Salas, Jesús Morlett-Chávez, Cristóbal N. Aguilar, Juan A. Ascacio-Valdés
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Sustainable Chemistry
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Online Access:https://www.mdpi.com/2673-4079/6/2/14
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author Christian Hernández-Hernández
Luis E. Estrada-Gil
Sonia A. Lozano-Sepúlveda
Ana M. Rivas-Estilla
Mayela Govea-Salas
Jesús Morlett-Chávez
Cristóbal N. Aguilar
Juan A. Ascacio-Valdés
author_facet Christian Hernández-Hernández
Luis E. Estrada-Gil
Sonia A. Lozano-Sepúlveda
Ana M. Rivas-Estilla
Mayela Govea-Salas
Jesús Morlett-Chávez
Cristóbal N. Aguilar
Juan A. Ascacio-Valdés
author_sort Christian Hernández-Hernández
collection DOAJ
description Rambutan peel is a great source of bioactive compounds, the same that, over the years, has been extracted using conventional technologies which have been proven to be harmful to the environment and potentially toxic to human beings. This study aimed to extract the same compounds using a hybridization of ultrasound/microwave extraction. The results were promising, as a total of 378.48 ± 9.19 mg/g of polyphenols were recovered from this procedure, and the most important molecules (geraniin, corilagin, and ellagic acid) were identified, giving this much more relevance. Furthermore, treatment with rambutan peel extract recovered with green technologies significantly reduced cell viability in HCV-infected liver cells. Notably, higher concentrations (4000 and 5000 ppm) led to more pronounced cell death in huh7 cells. The treatment also led to a significant reduction in viral protein and RNA expression in HCV-infected cells. These findings suggest that rambutan peel extract obtained from the combination of ultrasound and microwave extraction, particularly the ellagitannins present, have potential antiviral properties. Further research is needed to explore its mechanism of action and its potential as a therapeutic agent for Hepatitis C.
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issn 2673-4079
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publishDate 2025-04-01
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series Sustainable Chemistry
spelling doaj-art-ff2a9acd98f54fa2969ecb3a5594e4092025-08-20T03:27:43ZengMDPI AGSustainable Chemistry2673-40792025-04-01621410.3390/suschem6020014Antiviral Activity of Rambutan Peel Polyphenols Obtained Using Green Extraction Technology and SolventsChristian Hernández-Hernández0Luis E. Estrada-Gil1Sonia A. Lozano-Sepúlveda2Ana M. Rivas-Estilla3Mayela Govea-Salas4Jesús Morlett-Chávez5Cristóbal N. Aguilar6Juan A. Ascacio-Valdés7Bioprocesses & Bioproducts Research Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, MexicoBioprocesses & Bioproducts Research Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, MexicoCenter for Innovation and Research in Medical Virology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey 66450, Nuevo Leon, MexicoBiochemistry and Molecular Medicine Department, School of Medicine, Autonomous University of Nuevo Leon, Monterrey 66450, Nuevo Leon, MexicoLaboratory of Nanobiosciences, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, MexicoLaboratory of Molecular Biology, Health Research Department, School of Medicine, Autonomous University of Coahuila, Saltillo 25280, Coahuila, MexicoBioprocesses & Bioproducts Research Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, MexicoBioprocesses & Bioproducts Research Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, MexicoRambutan peel is a great source of bioactive compounds, the same that, over the years, has been extracted using conventional technologies which have been proven to be harmful to the environment and potentially toxic to human beings. This study aimed to extract the same compounds using a hybridization of ultrasound/microwave extraction. The results were promising, as a total of 378.48 ± 9.19 mg/g of polyphenols were recovered from this procedure, and the most important molecules (geraniin, corilagin, and ellagic acid) were identified, giving this much more relevance. Furthermore, treatment with rambutan peel extract recovered with green technologies significantly reduced cell viability in HCV-infected liver cells. Notably, higher concentrations (4000 and 5000 ppm) led to more pronounced cell death in huh7 cells. The treatment also led to a significant reduction in viral protein and RNA expression in HCV-infected cells. These findings suggest that rambutan peel extract obtained from the combination of ultrasound and microwave extraction, particularly the ellagitannins present, have potential antiviral properties. Further research is needed to explore its mechanism of action and its potential as a therapeutic agent for Hepatitis C.https://www.mdpi.com/2673-4079/6/2/14ellagitanninsmicrowave-/ultrasound-assisted extractionhybrid technologyantiproliferative
spellingShingle Christian Hernández-Hernández
Luis E. Estrada-Gil
Sonia A. Lozano-Sepúlveda
Ana M. Rivas-Estilla
Mayela Govea-Salas
Jesús Morlett-Chávez
Cristóbal N. Aguilar
Juan A. Ascacio-Valdés
Antiviral Activity of Rambutan Peel Polyphenols Obtained Using Green Extraction Technology and Solvents
Sustainable Chemistry
ellagitannins
microwave-/ultrasound-assisted extraction
hybrid technology
antiproliferative
title Antiviral Activity of Rambutan Peel Polyphenols Obtained Using Green Extraction Technology and Solvents
title_full Antiviral Activity of Rambutan Peel Polyphenols Obtained Using Green Extraction Technology and Solvents
title_fullStr Antiviral Activity of Rambutan Peel Polyphenols Obtained Using Green Extraction Technology and Solvents
title_full_unstemmed Antiviral Activity of Rambutan Peel Polyphenols Obtained Using Green Extraction Technology and Solvents
title_short Antiviral Activity of Rambutan Peel Polyphenols Obtained Using Green Extraction Technology and Solvents
title_sort antiviral activity of rambutan peel polyphenols obtained using green extraction technology and solvents
topic ellagitannins
microwave-/ultrasound-assisted extraction
hybrid technology
antiproliferative
url https://www.mdpi.com/2673-4079/6/2/14
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