Pharmaco-Technological Characterization, Structural Analysis, and Toxicological Evaluation of the Novel Polyene Antibiotic Roseofungin for Drug Development
<b>Background/Objectives:</b> pentane polyene antibiotic Roseofungin produced by actinomycetes possessing wide range of antimicrobial activity. <b>Methods:</b> The structure of novel polyene antibiotic Roseofungin was confirmed through FTIR, H<sup>1</sup> nuclear...
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2025-03-01
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| author | Amankeldy Sadanov Dmitriy Berillo Assya Bagimbayeva Gul Baimakhanova Liliya N. Ibragimova Iliyas Raikhanovich Kulmaganbetov Farida Nurmaganbetova Gulbany Sarsenbaeva Saltanat Orazymbet Baiken Baimakhanova Olga Lakh Diana Tleubekova Gulnara T. Dzhakibaeva Tulegen Mussaldinov |
| author_facet | Amankeldy Sadanov Dmitriy Berillo Assya Bagimbayeva Gul Baimakhanova Liliya N. Ibragimova Iliyas Raikhanovich Kulmaganbetov Farida Nurmaganbetova Gulbany Sarsenbaeva Saltanat Orazymbet Baiken Baimakhanova Olga Lakh Diana Tleubekova Gulnara T. Dzhakibaeva Tulegen Mussaldinov |
| author_sort | Amankeldy Sadanov |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> pentane polyene antibiotic Roseofungin produced by actinomycetes possessing wide range of antimicrobial activity. <b>Methods:</b> The structure of novel polyene antibiotic Roseofungin was confirmed through FTIR, H<sup>1</sup> nuclear magnetic resonance, and high-performance liquid chromatography analysis with a mass detector. The substance pharmaco-technological parameters were evaluated. Additionally, the in silico modelling of various physicochemical parameters and biological activities was performed using validated open-access software tools such as ProTox3, SwissADME, and ADMET SAR1. The evaluation of its toxicological profile was also investigated in vivo. <b>Results:</b> The Roseofungin exhibits potential risks in certain categories, including immunotoxicity, respiratory toxicity, and nephrotoxicity, as predicted in silico. However, Roseofungin shows a relatively safe profile with regard to hepatotoxicity, neurotoxicity, and mutagenicity, along with lower risks of carcinogenicity and cytotoxicity in silico. The analysis of body weight dynamics after Roseofungin exposure in mice revealed no statistically significant differences among the experimental groups. Similarly, in the absolute or relative weights of internal organs across the experimental groups after Roseofungin treatment, no significant differences were observed in vivo. Roseofungin appears as a light-yellow hygroscopic powder with a specific odour, possessing the ability to settle and classified as a light powder. The particles are lamellar crystals ranging in size from 3 μm to 6 μm, and the molecules generate electrostatic tension between themselves. The pharmaco-technological parameters of Roseofungin were comprehensively studied. <b>Conclusions:</b> The experimental data obtained provide a foundation for further pharmaceutical development of new drugs containing the original Roseofungin. |
| format | Article |
| id | doaj-art-ff255fe9da7645fe9f94ef2a27211619 |
| institution | DOAJ |
| issn | 1999-4923 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj-art-ff255fe9da7645fe9f94ef2a272116192025-08-20T03:13:32ZengMDPI AGPharmaceutics1999-49232025-03-0117443010.3390/pharmaceutics17040430Pharmaco-Technological Characterization, Structural Analysis, and Toxicological Evaluation of the Novel Polyene Antibiotic Roseofungin for Drug DevelopmentAmankeldy Sadanov0Dmitriy Berillo1Assya Bagimbayeva2Gul Baimakhanova3Liliya N. Ibragimova4Iliyas Raikhanovich Kulmaganbetov5Farida Nurmaganbetova6Gulbany Sarsenbaeva7Saltanat Orazymbet8Baiken Baimakhanova9Olga Lakh10Diana Tleubekova11Gulnara T. Dzhakibaeva12Tulegen Mussaldinov13Scientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanDepartment of Chemistry, M. Kozybayev North-Kazakhstan University, Zhumabaeva Str., 114, Petropavlovsk 150000, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanCenter for Pharmacy and Pharmacology of the Science and Technology Park, JSC Asfendiyarov Kazakh National Medical University, Almaty 050012, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, KazakhstanScientific Production Center for Microbiology and Virology LLP, Almaty 050010, Kazakhstan<b>Background/Objectives:</b> pentane polyene antibiotic Roseofungin produced by actinomycetes possessing wide range of antimicrobial activity. <b>Methods:</b> The structure of novel polyene antibiotic Roseofungin was confirmed through FTIR, H<sup>1</sup> nuclear magnetic resonance, and high-performance liquid chromatography analysis with a mass detector. The substance pharmaco-technological parameters were evaluated. Additionally, the in silico modelling of various physicochemical parameters and biological activities was performed using validated open-access software tools such as ProTox3, SwissADME, and ADMET SAR1. The evaluation of its toxicological profile was also investigated in vivo. <b>Results:</b> The Roseofungin exhibits potential risks in certain categories, including immunotoxicity, respiratory toxicity, and nephrotoxicity, as predicted in silico. However, Roseofungin shows a relatively safe profile with regard to hepatotoxicity, neurotoxicity, and mutagenicity, along with lower risks of carcinogenicity and cytotoxicity in silico. The analysis of body weight dynamics after Roseofungin exposure in mice revealed no statistically significant differences among the experimental groups. Similarly, in the absolute or relative weights of internal organs across the experimental groups after Roseofungin treatment, no significant differences were observed in vivo. Roseofungin appears as a light-yellow hygroscopic powder with a specific odour, possessing the ability to settle and classified as a light powder. The particles are lamellar crystals ranging in size from 3 μm to 6 μm, and the molecules generate electrostatic tension between themselves. The pharmaco-technological parameters of Roseofungin were comprehensively studied. <b>Conclusions:</b> The experimental data obtained provide a foundation for further pharmaceutical development of new drugs containing the original Roseofungin.https://www.mdpi.com/1999-4923/17/4/430roseofunginantibioticpharmaco-technological parametersFTIRHPLCNMR |
| spellingShingle | Amankeldy Sadanov Dmitriy Berillo Assya Bagimbayeva Gul Baimakhanova Liliya N. Ibragimova Iliyas Raikhanovich Kulmaganbetov Farida Nurmaganbetova Gulbany Sarsenbaeva Saltanat Orazymbet Baiken Baimakhanova Olga Lakh Diana Tleubekova Gulnara T. Dzhakibaeva Tulegen Mussaldinov Pharmaco-Technological Characterization, Structural Analysis, and Toxicological Evaluation of the Novel Polyene Antibiotic Roseofungin for Drug Development Pharmaceutics roseofungin antibiotic pharmaco-technological parameters FTIR HPLC NMR |
| title | Pharmaco-Technological Characterization, Structural Analysis, and Toxicological Evaluation of the Novel Polyene Antibiotic Roseofungin for Drug Development |
| title_full | Pharmaco-Technological Characterization, Structural Analysis, and Toxicological Evaluation of the Novel Polyene Antibiotic Roseofungin for Drug Development |
| title_fullStr | Pharmaco-Technological Characterization, Structural Analysis, and Toxicological Evaluation of the Novel Polyene Antibiotic Roseofungin for Drug Development |
| title_full_unstemmed | Pharmaco-Technological Characterization, Structural Analysis, and Toxicological Evaluation of the Novel Polyene Antibiotic Roseofungin for Drug Development |
| title_short | Pharmaco-Technological Characterization, Structural Analysis, and Toxicological Evaluation of the Novel Polyene Antibiotic Roseofungin for Drug Development |
| title_sort | pharmaco technological characterization structural analysis and toxicological evaluation of the novel polyene antibiotic roseofungin for drug development |
| topic | roseofungin antibiotic pharmaco-technological parameters FTIR HPLC NMR |
| url | https://www.mdpi.com/1999-4923/17/4/430 |
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