Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo
Stable vitiligo significantly impacts patients’ quality of life and presents a considerable challenge to healthcare providers. In recent years, cell therapy has emerged as a promising treatment for stable vitiligo, which is demonstrated encouraging results. Among current cell-based therapies, autolo...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1583271/full |
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| author | Qianren Zheng Jie Chen Yixun Huang Weikai Chen Dandan Cheng Qianqian Jia Meiqin Zhu Youguo Liao Qiulin He Qiulin He Shunli Wu Shunli Wu |
| author_facet | Qianren Zheng Jie Chen Yixun Huang Weikai Chen Dandan Cheng Qianqian Jia Meiqin Zhu Youguo Liao Qiulin He Qiulin He Shunli Wu Shunli Wu |
| author_sort | Qianren Zheng |
| collection | DOAJ |
| description | Stable vitiligo significantly impacts patients’ quality of life and presents a considerable challenge to healthcare providers. In recent years, cell therapy has emerged as a promising treatment for stable vitiligo, which is demonstrated encouraging results. Among current cell-based therapies, autologous epidermal cell transplantation is regarded as a safe and cost-effective strategy. However, the therapeutic outcome critically depends on the retention and viability of the transplanted cells at the target site. Therefore, there is an urgent need to develop novel strategies to improve cell retention and maintain cell viability for improving therapeutic efficacy. In this work, a novel cell extraction method was first developed with deal with 2 h at 37°C to obtain epidermal cells while maintaining high cell viability. Subsequently, the crosslinked hyaluronic acid (HA) by BDDE was utilized as 3D scaffold for cell delivery to treat stable vitiligo. By combining the new extraction method with the HA-based hydrogel scaffold, we achieved prolonged cell retention without compromising cell viability. This approach provides a promising, time-saving strategy for treating stable vitiligo using autologous epidermal cells. |
| format | Article |
| id | doaj-art-ff193b20c9f14e2bb5a43c05d84505e1 |
| institution | Kabale University |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj-art-ff193b20c9f14e2bb5a43c05d84505e12025-08-20T03:53:12ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-05-011210.3389/fmed.2025.15832711583271Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligoQianren Zheng0Jie Chen1Yixun Huang2Weikai Chen3Dandan Cheng4Qianqian Jia5Meiqin Zhu6Youguo Liao7Qiulin He8Qiulin He9Shunli Wu10Shunli Wu11Hangzhou Singclean Medical Products Co., Ltd, Hangzhou, ChinaDepartment of Student Affairs, Jiaxing Vocational Technical College, Jiaxing, Zhejiang, ChinaDepartment of Orthopedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, ChinaDepartment of Orthopedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, ChinaDepartment of Gynecological Oncology, Wenzhou Central Hospital, Wenzhou, Zhejiang, ChinaHangzhou Singclean Medical Products Co., Ltd, Hangzhou, ChinaHangzhou Singclean Medical Products Co., Ltd, Hangzhou, ChinaDepartment of Burns and Wound Care Center, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaHangzhou Singclean Medical Products Co., Ltd, Hangzhou, ChinaDepartment of Macromolecular Science, Fudan University, Shanghai, ChinaHangzhou Singclean Medical Products Co., Ltd, Hangzhou, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao, Shandong, ChinaStable vitiligo significantly impacts patients’ quality of life and presents a considerable challenge to healthcare providers. In recent years, cell therapy has emerged as a promising treatment for stable vitiligo, which is demonstrated encouraging results. Among current cell-based therapies, autologous epidermal cell transplantation is regarded as a safe and cost-effective strategy. However, the therapeutic outcome critically depends on the retention and viability of the transplanted cells at the target site. Therefore, there is an urgent need to develop novel strategies to improve cell retention and maintain cell viability for improving therapeutic efficacy. In this work, a novel cell extraction method was first developed with deal with 2 h at 37°C to obtain epidermal cells while maintaining high cell viability. Subsequently, the crosslinked hyaluronic acid (HA) by BDDE was utilized as 3D scaffold for cell delivery to treat stable vitiligo. By combining the new extraction method with the HA-based hydrogel scaffold, we achieved prolonged cell retention without compromising cell viability. This approach provides a promising, time-saving strategy for treating stable vitiligo using autologous epidermal cells.https://www.frontiersin.org/articles/10.3389/fmed.2025.1583271/fullautologous epidermal melanocytescrosslinked hyaluronic acid hydrogelcell therapystable vitiligonude mice |
| spellingShingle | Qianren Zheng Jie Chen Yixun Huang Weikai Chen Dandan Cheng Qianqian Jia Meiqin Zhu Youguo Liao Qiulin He Qiulin He Shunli Wu Shunli Wu Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo Frontiers in Medicine autologous epidermal melanocytes crosslinked hyaluronic acid hydrogel cell therapy stable vitiligo nude mice |
| title | Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo |
| title_full | Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo |
| title_fullStr | Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo |
| title_full_unstemmed | Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo |
| title_short | Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo |
| title_sort | injectable crosslinked ha hydrogel a promising carrier for cell transplantation to treat stable vitiligo |
| topic | autologous epidermal melanocytes crosslinked hyaluronic acid hydrogel cell therapy stable vitiligo nude mice |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1583271/full |
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