Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde
Mushrooms have been used as food and medicinal purposes for centuries. In this study, some edible Ascomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the samples were performed afterward. PCR products were sent for sequence analysis after ITS fragments were am...
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| Format: | Article |
| Language: | English |
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Hasan Eleroğlu
2021-11-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4642 |
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| author | Şükrü Canpolat Ilgaz Akata Yasemin İşlek Elif Canpolat Cemil İşlek |
| author_facet | Şükrü Canpolat Ilgaz Akata Yasemin İşlek Elif Canpolat Cemil İşlek |
| author_sort | Şükrü Canpolat |
| collection | DOAJ |
| description | Mushrooms have been used as food and medicinal purposes for centuries. In this study, some edible Ascomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the samples were performed afterward. PCR products were sent for sequence analysis after ITS fragments were amplified with suitable primers concerning DNA isolation of samples. The obtained data were compared with the Genbank database for samples’ determination. The mushroom samples were determined to be Terfezia claveryi Chatin and Morchella esculenta (L.) Pers. Samples were extracted by ethanol and methanol to determine antimicrobial activity using the disc diffusion method. Antimicrobial zones against microorganisms were measured. Then DPPH radical scavenging effects and total phenolic content of T. claveryi and M. esculenta mushroom samples were studied. The highest antimicrobial activity was observed in ethanol extracts of M. esculenta. When the DPPH radical scavenging activities are taken into account, the activity is increased depending on the concentration. The highest DPPH scavenging activity was found in the extract of M. esculenta with 40.86%. It was determined that the total phenolic substances in methanol extracts of mushroom samples varies between 533.28 ± 1,15-537.34 ±2.20 μg GAE / mg extract. |
| format | Article |
| id | doaj-art-ff135b638b794df29fb077235dda7f3d |
| institution | OA Journals |
| issn | 2148-127X |
| language | English |
| publishDate | 2021-11-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-ff135b638b794df29fb077235dda7f3d2025-08-20T02:04:49ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2021-11-019101915192010.24925/turjaf.v9i10.1915-1920.46422234Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from NiğdeŞükrü Canpolat0Ilgaz Akata1Yasemin İşlek2Elif Canpolat3Cemil İşlek4Central Laboratory Research Center, Niğde Ömer Halisdemir University, 51240 NiğdeDepartment of Biology, Faculty of Science, Ankara University, 06560AnkaraDepartment of Electronics and Automation, Vocational School of Technical Sciences, Niğde Ömer Halisdemir University, 51240 NiğdeDeparment of Biotechnology, Faculty of Arts and Science, Niğde Ömer Halisdemir University, 51240 NiğdeDeparment of Biotechnology, Faculty of Arts and Science, Niğde Ömer Halisdemir University, 51240 NiğdeMushrooms have been used as food and medicinal purposes for centuries. In this study, some edible Ascomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the samples were performed afterward. PCR products were sent for sequence analysis after ITS fragments were amplified with suitable primers concerning DNA isolation of samples. The obtained data were compared with the Genbank database for samples’ determination. The mushroom samples were determined to be Terfezia claveryi Chatin and Morchella esculenta (L.) Pers. Samples were extracted by ethanol and methanol to determine antimicrobial activity using the disc diffusion method. Antimicrobial zones against microorganisms were measured. Then DPPH radical scavenging effects and total phenolic content of T. claveryi and M. esculenta mushroom samples were studied. The highest antimicrobial activity was observed in ethanol extracts of M. esculenta. When the DPPH radical scavenging activities are taken into account, the activity is increased depending on the concentration. The highest DPPH scavenging activity was found in the extract of M. esculenta with 40.86%. It was determined that the total phenolic substances in methanol extracts of mushroom samples varies between 533.28 ± 1,15-537.34 ±2.20 μg GAE / mg extract.http://www.agrifoodscience.com/index.php/TURJAF/article/view/4642t. claveryim. esculentaantimicrobial activityantioxidantnigde |
| spellingShingle | Şükrü Canpolat Ilgaz Akata Yasemin İşlek Elif Canpolat Cemil İşlek Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde Turkish Journal of Agriculture: Food Science and Technology t. claveryi m. esculenta antimicrobial activity antioxidant nigde |
| title | Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde |
| title_full | Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde |
| title_fullStr | Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde |
| title_full_unstemmed | Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde |
| title_short | Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde |
| title_sort | total phenolics antioxidative and antimicrobial activities of some edible ascomycota collected from nigde |
| topic | t. claveryi m. esculenta antimicrobial activity antioxidant nigde |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4642 |
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