The Evaluation of <i>Citrus bergamia</i> Phytochemicals as Potential Cholesterol-Lowering Agents against HMG-CoA Reductase: An In Silico Molecular Docking Study

Elevated cholesterol levels, or hypercholesterolemia, have been recognized as the underlying cause of various diseases, most notably cardiovascular diseases. Unfortunately, most cholesterol-lowering (or anti-hypercholesterolemic) drugs are associated with several adverse effects, emphasizing the nee...

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Main Authors: Nesteve John Agosto, Patricia Blanch Alambatin, Joemer Bacalso, JC Cabisada, Beatriz Danica Carating
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Biology and Life Sciences Forum
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Online Access:https://www.mdpi.com/2673-9976/35/1/7
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author Nesteve John Agosto
Patricia Blanch Alambatin
Joemer Bacalso
JC Cabisada
Beatriz Danica Carating
author_facet Nesteve John Agosto
Patricia Blanch Alambatin
Joemer Bacalso
JC Cabisada
Beatriz Danica Carating
author_sort Nesteve John Agosto
collection DOAJ
description Elevated cholesterol levels, or hypercholesterolemia, have been recognized as the underlying cause of various diseases, most notably cardiovascular diseases. Unfortunately, most cholesterol-lowering (or anti-hypercholesterolemic) drugs are associated with several adverse effects, emphasizing the need to identify new cholesterol-lowering strategies. Natural products, particularly bioactive phytochemicals, have gained significant attention for their safer profile, fewer side effects, and potential health benefits, including cholesterol-lowering properties. The citrus fruit bergamot (<i>Citrus bergamia</i>) is renowned for its diverse array of bioactive phytochemicals. In this study, an in silico approach was utilized to assess the cholesterol-lowering potential of phytochemicals derived from <i>C. bergamia</i>. Molecular docking using AutoDock Vina of the selected phytochemicals was performed against the HMG-CoA reductase (HMGR), an enzyme targeted for hypercholesterolemia. Our results indicated that among the selected 20 phytochemicals, 8, namely eriocitrin, narirutin, scolymoside, neodiosmin, brutieridin, neohesperidin, rhoifolin, and naringin, exhibited better binding affinities than the conventional HMGR inhibitor, atorvastatin (−9.2 kcal/mol). Notably, among these top eight phytochemicals, eriocitrin displayed the most favorable binding affinity of −10.0 kcal/mol. These findings strongly imply that <i>C. bergamia</i> possesses potential HMGR-inhibitory activity and anti-hypercholesterolemic activity, primarily due to the high binding affinities exhibited by its phytochemical constituents. Therefore, further studies must be considered to comprehensively explore the cholesterol-lowering properties of <i>C. bergamia</i> phytochemicals.
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spelling doaj-art-ff0e71fbae6c424cadbb2d4032712dde2025-08-20T02:11:01ZengMDPI AGBiology and Life Sciences Forum2673-99762024-09-01351710.3390/blsf2024035007The Evaluation of <i>Citrus bergamia</i> Phytochemicals as Potential Cholesterol-Lowering Agents against HMG-CoA Reductase: An In Silico Molecular Docking StudyNesteve John Agosto0Patricia Blanch Alambatin1Joemer Bacalso2JC Cabisada3Beatriz Danica Carating4Center for Natural Products Research, University of Science and Technology of Southern Philippines, Cagayan de Oro City 9000, PhilippinesDepartment of Chemistry, University of Science and Technology of Southern Philippines, Cagayan de Oro City 9000, PhilippinesDepartment of Chemistry, University of Science and Technology of Southern Philippines, Cagayan de Oro City 9000, PhilippinesDepartment of Chemistry, University of Science and Technology of Southern Philippines, Cagayan de Oro City 9000, PhilippinesDepartment of Chemistry, University of Science and Technology of Southern Philippines, Cagayan de Oro City 9000, PhilippinesElevated cholesterol levels, or hypercholesterolemia, have been recognized as the underlying cause of various diseases, most notably cardiovascular diseases. Unfortunately, most cholesterol-lowering (or anti-hypercholesterolemic) drugs are associated with several adverse effects, emphasizing the need to identify new cholesterol-lowering strategies. Natural products, particularly bioactive phytochemicals, have gained significant attention for their safer profile, fewer side effects, and potential health benefits, including cholesterol-lowering properties. The citrus fruit bergamot (<i>Citrus bergamia</i>) is renowned for its diverse array of bioactive phytochemicals. In this study, an in silico approach was utilized to assess the cholesterol-lowering potential of phytochemicals derived from <i>C. bergamia</i>. Molecular docking using AutoDock Vina of the selected phytochemicals was performed against the HMG-CoA reductase (HMGR), an enzyme targeted for hypercholesterolemia. Our results indicated that among the selected 20 phytochemicals, 8, namely eriocitrin, narirutin, scolymoside, neodiosmin, brutieridin, neohesperidin, rhoifolin, and naringin, exhibited better binding affinities than the conventional HMGR inhibitor, atorvastatin (−9.2 kcal/mol). Notably, among these top eight phytochemicals, eriocitrin displayed the most favorable binding affinity of −10.0 kcal/mol. These findings strongly imply that <i>C. bergamia</i> possesses potential HMGR-inhibitory activity and anti-hypercholesterolemic activity, primarily due to the high binding affinities exhibited by its phytochemical constituents. Therefore, further studies must be considered to comprehensively explore the cholesterol-lowering properties of <i>C. bergamia</i> phytochemicals.https://www.mdpi.com/2673-9976/35/1/7cholesterol-lowering propertiesCitrus bergamiaHMG-CoA reductasemolecular dockingin silico
spellingShingle Nesteve John Agosto
Patricia Blanch Alambatin
Joemer Bacalso
JC Cabisada
Beatriz Danica Carating
The Evaluation of <i>Citrus bergamia</i> Phytochemicals as Potential Cholesterol-Lowering Agents against HMG-CoA Reductase: An In Silico Molecular Docking Study
Biology and Life Sciences Forum
cholesterol-lowering properties
Citrus bergamia
HMG-CoA reductase
molecular docking
in silico
title The Evaluation of <i>Citrus bergamia</i> Phytochemicals as Potential Cholesterol-Lowering Agents against HMG-CoA Reductase: An In Silico Molecular Docking Study
title_full The Evaluation of <i>Citrus bergamia</i> Phytochemicals as Potential Cholesterol-Lowering Agents against HMG-CoA Reductase: An In Silico Molecular Docking Study
title_fullStr The Evaluation of <i>Citrus bergamia</i> Phytochemicals as Potential Cholesterol-Lowering Agents against HMG-CoA Reductase: An In Silico Molecular Docking Study
title_full_unstemmed The Evaluation of <i>Citrus bergamia</i> Phytochemicals as Potential Cholesterol-Lowering Agents against HMG-CoA Reductase: An In Silico Molecular Docking Study
title_short The Evaluation of <i>Citrus bergamia</i> Phytochemicals as Potential Cholesterol-Lowering Agents against HMG-CoA Reductase: An In Silico Molecular Docking Study
title_sort evaluation of i citrus bergamia i phytochemicals as potential cholesterol lowering agents against hmg coa reductase an in silico molecular docking study
topic cholesterol-lowering properties
Citrus bergamia
HMG-CoA reductase
molecular docking
in silico
url https://www.mdpi.com/2673-9976/35/1/7
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