Anti-Inflammatory Activity of Thymol and Thymol-Rich Essential Oils: Mechanisms, Applications, and Recent Findings

Thymol, a monoterpenoid phenol present in the essential oils of several aromatic plants, has attracted considerable attention for its anti-inflammatory effects, often in combination with other bioactive compounds. This work explores the mechanisms behind the anti-inflammatory activity of thymol and...

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Main Authors: Custódia Gago, Ana Serralheiro, Maria da Graça Miguel
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/30/11/2450
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author Custódia Gago
Ana Serralheiro
Maria da Graça Miguel
author_facet Custódia Gago
Ana Serralheiro
Maria da Graça Miguel
author_sort Custódia Gago
collection DOAJ
description Thymol, a monoterpenoid phenol present in the essential oils of several aromatic plants, has attracted considerable attention for its anti-inflammatory effects, often in combination with other bioactive compounds. This work explores the mechanisms behind the anti-inflammatory activity of thymol and thymol-rich essential oils, summarizing recent experimental findings. Inflammation, a key factor in numerous chronic diseases, can be modulated by targeting essential molecular pathways, such as MAPK, NF-<i>κ</i>B, JAK/STAT, and arachidonic acid signaling. Thymol has been shown to influence these pathways, reducing the production of pro-inflammatory cytokines and mediators. Beyond its anti-inflammatory effects, thymol also exhibits a broad range of biological activities, including antimicrobial, antioxidant, and anticancer properties. The applications of thymol and thymol-containing essential oils in therapeutic formulations, food additives, and veterinary medicine are also reviewed. Despite promising preclinical results, challenges such as low bioavailability and toxicity at high doses limit their clinical use. Recent developments in drug delivery systems, such as encapsulation in micro- and nanoparticles, are suggested as strategies to enhance efficacy. Additionally, the synergistic effects of thymol with other natural products are examined, offering the potential for improved therapeutic outcomes.
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spelling doaj-art-ff0dd03a1e43483683b527a3171e8ef82025-08-20T02:32:52ZengMDPI AGMolecules1420-30492025-06-013011245010.3390/molecules30112450Anti-Inflammatory Activity of Thymol and Thymol-Rich Essential Oils: Mechanisms, Applications, and Recent FindingsCustódia Gago0Ana Serralheiro1Maria da Graça Miguel2MED—Mediterranean Institute for Agriculture, Environment and Development & CHANGE-Global Change and Sustainability Institute, FCT, Universidade do Algarve, Edf. 8, Campus de Gambelas, 8005-139 Faro, PortugalCCMAR—Centre for Marine Sciences, FCT, Universidade do Algarve, Edf. 2, Campus de Gambelas, 8005-139 Faro, PortugalMED—Mediterranean Institute for Agriculture, Environment and Development & CHANGE-Global Change and Sustainability Institute, FCT, Universidade do Algarve, Edf. 8, Campus de Gambelas, 8005-139 Faro, PortugalThymol, a monoterpenoid phenol present in the essential oils of several aromatic plants, has attracted considerable attention for its anti-inflammatory effects, often in combination with other bioactive compounds. This work explores the mechanisms behind the anti-inflammatory activity of thymol and thymol-rich essential oils, summarizing recent experimental findings. Inflammation, a key factor in numerous chronic diseases, can be modulated by targeting essential molecular pathways, such as MAPK, NF-<i>κ</i>B, JAK/STAT, and arachidonic acid signaling. Thymol has been shown to influence these pathways, reducing the production of pro-inflammatory cytokines and mediators. Beyond its anti-inflammatory effects, thymol also exhibits a broad range of biological activities, including antimicrobial, antioxidant, and anticancer properties. The applications of thymol and thymol-containing essential oils in therapeutic formulations, food additives, and veterinary medicine are also reviewed. Despite promising preclinical results, challenges such as low bioavailability and toxicity at high doses limit their clinical use. Recent developments in drug delivery systems, such as encapsulation in micro- and nanoparticles, are suggested as strategies to enhance efficacy. Additionally, the synergistic effects of thymol with other natural products are examined, offering the potential for improved therapeutic outcomes.https://www.mdpi.com/1420-3049/30/11/2450in vitroin vivomechanismsynergismencapsulationchemotherapy
spellingShingle Custódia Gago
Ana Serralheiro
Maria da Graça Miguel
Anti-Inflammatory Activity of Thymol and Thymol-Rich Essential Oils: Mechanisms, Applications, and Recent Findings
Molecules
in vitro
in vivo
mechanism
synergism
encapsulation
chemotherapy
title Anti-Inflammatory Activity of Thymol and Thymol-Rich Essential Oils: Mechanisms, Applications, and Recent Findings
title_full Anti-Inflammatory Activity of Thymol and Thymol-Rich Essential Oils: Mechanisms, Applications, and Recent Findings
title_fullStr Anti-Inflammatory Activity of Thymol and Thymol-Rich Essential Oils: Mechanisms, Applications, and Recent Findings
title_full_unstemmed Anti-Inflammatory Activity of Thymol and Thymol-Rich Essential Oils: Mechanisms, Applications, and Recent Findings
title_short Anti-Inflammatory Activity of Thymol and Thymol-Rich Essential Oils: Mechanisms, Applications, and Recent Findings
title_sort anti inflammatory activity of thymol and thymol rich essential oils mechanisms applications and recent findings
topic in vitro
in vivo
mechanism
synergism
encapsulation
chemotherapy
url https://www.mdpi.com/1420-3049/30/11/2450
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AT anaserralheiro antiinflammatoryactivityofthymolandthymolrichessentialoilsmechanismsapplicationsandrecentfindings
AT mariadagracamiguel antiinflammatoryactivityofthymolandthymolrichessentialoilsmechanismsapplicationsandrecentfindings