Gibberellins treatment or stratification can break dormancy of the seeds of three Ranunculus species native to Korea

Abstract In this study, the seed germination and dormancy characteristics of three Ranunculus species native to Korea (R. cantoniensis, R. chinensis, and R. sceleratus) were determined to establish an effective dormancy-breaking protocol. The results revealed that the R. cantoniensis, R. chinensis,...

Full description

Saved in:
Bibliographic Details
Main Authors: Soyeon Park, Kyungtae Park, Jihun Lee, Hamin Lee, Bo-Kook Jang, Hayan Lee, Ju-Sung Cho
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-024-80159-x
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850128902272319488
author Soyeon Park
Kyungtae Park
Jihun Lee
Hamin Lee
Bo-Kook Jang
Hayan Lee
Ju-Sung Cho
author_facet Soyeon Park
Kyungtae Park
Jihun Lee
Hamin Lee
Bo-Kook Jang
Hayan Lee
Ju-Sung Cho
author_sort Soyeon Park
collection DOAJ
description Abstract In this study, the seed germination and dormancy characteristics of three Ranunculus species native to Korea (R. cantoniensis, R. chinensis, and R. sceleratus) were determined to establish an effective dormancy-breaking protocol. The results revealed that the R. cantoniensis, R. chinensis, and R. sceleratus seeds responded differently to treatment with gibberellins (GAs) and stratification. Warm stratification at 20℃ increased the germination percentage and partially broke the dormancy of the R. cantoniensis seeds; moreover, the germination temperature range of the seeds expanded upon treatment with high-concentration GA4 + 7. Meanwhile, neither cold nor warm stratification was effective at breaking the dormancy of R. chinensis seeds; however, high-concentration GA4 + 7 treatment broke the dormancy of the seeds by expanding the germination temperature range. Lastly, the R. sceleratus seeds did not respond to the treatment with GAs; however, their germination percentage increased after undergoing warm stratification at 25℃. The seeds of these three species were characterized as exhibiting nondeep morphophysiological dormancy. The findings of this study enhance our understanding of the temperature-related germination responses of the three Ranunculus species, offering valuable insights into their conservation and utilization.
format Article
id doaj-art-ff0a3b299be8447a84c662cd67994e62
institution OA Journals
issn 2045-2322
language English
publishDate 2024-11-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-ff0a3b299be8447a84c662cd67994e622025-08-20T02:33:08ZengNature PortfolioScientific Reports2045-23222024-11-0114111410.1038/s41598-024-80159-xGibberellins treatment or stratification can break dormancy of the seeds of three Ranunculus species native to KoreaSoyeon Park0Kyungtae Park1Jihun Lee2Hamin Lee3Bo-Kook Jang4Hayan Lee5Ju-Sung Cho6Division of Animal, Horticultural and Food Sciences, Chungbuk National UniversityDivision of Animal, Horticultural and Food Sciences, Chungbuk National UniversityDepartment of DMZ Forest Biological Resources Conservation, Korea National ArboretumDivision of Animal, Horticultural and Food Sciences, Chungbuk National UniversityDepartment of Horticulture, Sunchon National UniversityForest Ecosystem Restoration Division, Baekdudaegan National ArboretumDivision of Animal, Horticultural and Food Sciences, Chungbuk National UniversityAbstract In this study, the seed germination and dormancy characteristics of three Ranunculus species native to Korea (R. cantoniensis, R. chinensis, and R. sceleratus) were determined to establish an effective dormancy-breaking protocol. The results revealed that the R. cantoniensis, R. chinensis, and R. sceleratus seeds responded differently to treatment with gibberellins (GAs) and stratification. Warm stratification at 20℃ increased the germination percentage and partially broke the dormancy of the R. cantoniensis seeds; moreover, the germination temperature range of the seeds expanded upon treatment with high-concentration GA4 + 7. Meanwhile, neither cold nor warm stratification was effective at breaking the dormancy of R. chinensis seeds; however, high-concentration GA4 + 7 treatment broke the dormancy of the seeds by expanding the germination temperature range. Lastly, the R. sceleratus seeds did not respond to the treatment with GAs; however, their germination percentage increased after undergoing warm stratification at 25℃. The seeds of these three species were characterized as exhibiting nondeep morphophysiological dormancy. The findings of this study enhance our understanding of the temperature-related germination responses of the three Ranunculus species, offering valuable insights into their conservation and utilization.https://doi.org/10.1038/s41598-024-80159-xEnzyme activityExpansion of germination temperature rangeGA4 + 7Nondeep MPDStratification
spellingShingle Soyeon Park
Kyungtae Park
Jihun Lee
Hamin Lee
Bo-Kook Jang
Hayan Lee
Ju-Sung Cho
Gibberellins treatment or stratification can break dormancy of the seeds of three Ranunculus species native to Korea
Scientific Reports
Enzyme activity
Expansion of germination temperature range
GA4 + 7
Nondeep MPD
Stratification
title Gibberellins treatment or stratification can break dormancy of the seeds of three Ranunculus species native to Korea
title_full Gibberellins treatment or stratification can break dormancy of the seeds of three Ranunculus species native to Korea
title_fullStr Gibberellins treatment or stratification can break dormancy of the seeds of three Ranunculus species native to Korea
title_full_unstemmed Gibberellins treatment or stratification can break dormancy of the seeds of three Ranunculus species native to Korea
title_short Gibberellins treatment or stratification can break dormancy of the seeds of three Ranunculus species native to Korea
title_sort gibberellins treatment or stratification can break dormancy of the seeds of three ranunculus species native to korea
topic Enzyme activity
Expansion of germination temperature range
GA4 + 7
Nondeep MPD
Stratification
url https://doi.org/10.1038/s41598-024-80159-x
work_keys_str_mv AT soyeonpark gibberellinstreatmentorstratificationcanbreakdormancyoftheseedsofthreeranunculusspeciesnativetokorea
AT kyungtaepark gibberellinstreatmentorstratificationcanbreakdormancyoftheseedsofthreeranunculusspeciesnativetokorea
AT jihunlee gibberellinstreatmentorstratificationcanbreakdormancyoftheseedsofthreeranunculusspeciesnativetokorea
AT haminlee gibberellinstreatmentorstratificationcanbreakdormancyoftheseedsofthreeranunculusspeciesnativetokorea
AT bokookjang gibberellinstreatmentorstratificationcanbreakdormancyoftheseedsofthreeranunculusspeciesnativetokorea
AT hayanlee gibberellinstreatmentorstratificationcanbreakdormancyoftheseedsofthreeranunculusspeciesnativetokorea
AT jusungcho gibberellinstreatmentorstratificationcanbreakdormancyoftheseedsofthreeranunculusspeciesnativetokorea