Unique North American isolates of severe metastatic hypervirulent Klebsiella pneumoniae strain infections with hepatic abscesses seen in young patients within Texas.
<h4>Rationale</h4>Hypervirulent Klebsiella pneumoniae (hvKp) infections have principally been identified in Asia. Within a two-month period, two patients between the ages of 30 to 50 years old presented to a tertiary referral hospital in Texas with septic shock, hepatic abscess, and sept...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0308305&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823864130387312640 |
|---|---|
| author | Junaid M Alam Eric N Riha Haris Ahmed Hong M Thai Kevin Garnepudi Ramesh B Kesavan Gnananandh Jayaraman Anna Sangster Dylan Curry Heidi A Butz Lori Smith Maureen Vowles Kelly F Oakeson Erin L Young Siva T Sarva |
| author_facet | Junaid M Alam Eric N Riha Haris Ahmed Hong M Thai Kevin Garnepudi Ramesh B Kesavan Gnananandh Jayaraman Anna Sangster Dylan Curry Heidi A Butz Lori Smith Maureen Vowles Kelly F Oakeson Erin L Young Siva T Sarva |
| author_sort | Junaid M Alam |
| collection | DOAJ |
| description | <h4>Rationale</h4>Hypervirulent Klebsiella pneumoniae (hvKp) infections have principally been identified in Asia. Within a two-month period, two patients between the ages of 30 to 50 years old presented to a tertiary referral hospital in Texas with septic shock, hepatic abscess, and septic thrombophlebitis. Blood cultures were positive for Klebsiella pneumoniae (isolates 2020CK-00441 and 2021CK-00720 respectively). The first patient survived after a prolonged hospital course while the second patient expired.<h4>Objectives</h4>Describe the clinical presentation of these two patients. Perform whole genome sequencing and bioinformatic analysis to evaluate potential outbreak of specific hvKp bacteria isolates.<h4>Methods</h4>Whole genome sequencing was performed using both paired-end Illumina MiSeq and nanopore sequencing to obtain a Completed genome for both isolates.<h4>Main results</h4>2020CK-00441 belonged to ST23 type while 2021CK-00720 was a ST65 type isolate. Kleborate analyses predicted with high confidence both isolates were hvKp. Phylogenetic analyses showed the two strains are not closely related to each other nor to any known hvKp isolates reported. Both isolates had yersiniabactin, colibactin, aerobactin and salmochelin producing loci which likely confer these isolates hvKp phenotype. 2020CK-00441 and 2021CK-00720 had a unique pK2044 like plasmid.<h4>Conclusions</h4>HvKp strains capable of causing devastating metastatic septic infections have emerged in Texas. These isolates are unique compared to other hvKp strains globally. Country-wide surveillance and whole genome sequencing of these strains is essential to prevent a major public health emergency in the USA. |
| format | Article |
| id | doaj-art-feecd735ad6a4354aae3945339db0bf2 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-feecd735ad6a4354aae3945339db0bf22025-02-09T05:30:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e030830510.1371/journal.pone.0308305Unique North American isolates of severe metastatic hypervirulent Klebsiella pneumoniae strain infections with hepatic abscesses seen in young patients within Texas.Junaid M AlamEric N RihaHaris AhmedHong M ThaiKevin GarnepudiRamesh B KesavanGnananandh JayaramanAnna SangsterDylan CurryHeidi A ButzLori SmithMaureen VowlesKelly F OakesonErin L YoungSiva T Sarva<h4>Rationale</h4>Hypervirulent Klebsiella pneumoniae (hvKp) infections have principally been identified in Asia. Within a two-month period, two patients between the ages of 30 to 50 years old presented to a tertiary referral hospital in Texas with septic shock, hepatic abscess, and septic thrombophlebitis. Blood cultures were positive for Klebsiella pneumoniae (isolates 2020CK-00441 and 2021CK-00720 respectively). The first patient survived after a prolonged hospital course while the second patient expired.<h4>Objectives</h4>Describe the clinical presentation of these two patients. Perform whole genome sequencing and bioinformatic analysis to evaluate potential outbreak of specific hvKp bacteria isolates.<h4>Methods</h4>Whole genome sequencing was performed using both paired-end Illumina MiSeq and nanopore sequencing to obtain a Completed genome for both isolates.<h4>Main results</h4>2020CK-00441 belonged to ST23 type while 2021CK-00720 was a ST65 type isolate. Kleborate analyses predicted with high confidence both isolates were hvKp. Phylogenetic analyses showed the two strains are not closely related to each other nor to any known hvKp isolates reported. Both isolates had yersiniabactin, colibactin, aerobactin and salmochelin producing loci which likely confer these isolates hvKp phenotype. 2020CK-00441 and 2021CK-00720 had a unique pK2044 like plasmid.<h4>Conclusions</h4>HvKp strains capable of causing devastating metastatic septic infections have emerged in Texas. These isolates are unique compared to other hvKp strains globally. Country-wide surveillance and whole genome sequencing of these strains is essential to prevent a major public health emergency in the USA.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0308305&type=printable |
| spellingShingle | Junaid M Alam Eric N Riha Haris Ahmed Hong M Thai Kevin Garnepudi Ramesh B Kesavan Gnananandh Jayaraman Anna Sangster Dylan Curry Heidi A Butz Lori Smith Maureen Vowles Kelly F Oakeson Erin L Young Siva T Sarva Unique North American isolates of severe metastatic hypervirulent Klebsiella pneumoniae strain infections with hepatic abscesses seen in young patients within Texas. PLoS ONE |
| title | Unique North American isolates of severe metastatic hypervirulent Klebsiella pneumoniae strain infections with hepatic abscesses seen in young patients within Texas. |
| title_full | Unique North American isolates of severe metastatic hypervirulent Klebsiella pneumoniae strain infections with hepatic abscesses seen in young patients within Texas. |
| title_fullStr | Unique North American isolates of severe metastatic hypervirulent Klebsiella pneumoniae strain infections with hepatic abscesses seen in young patients within Texas. |
| title_full_unstemmed | Unique North American isolates of severe metastatic hypervirulent Klebsiella pneumoniae strain infections with hepatic abscesses seen in young patients within Texas. |
| title_short | Unique North American isolates of severe metastatic hypervirulent Klebsiella pneumoniae strain infections with hepatic abscesses seen in young patients within Texas. |
| title_sort | unique north american isolates of severe metastatic hypervirulent klebsiella pneumoniae strain infections with hepatic abscesses seen in young patients within texas |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0308305&type=printable |
| work_keys_str_mv | AT junaidmalam uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT ericnriha uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT harisahmed uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT hongmthai uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT kevingarnepudi uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT rameshbkesavan uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT gnananandhjayaraman uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT annasangster uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT dylancurry uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT heidiabutz uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT lorismith uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT maureenvowles uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT kellyfoakeson uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT erinlyoung uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas AT sivatsarva uniquenorthamericanisolatesofseveremetastatichypervirulentklebsiellapneumoniaestraininfectionswithhepaticabscessesseeninyoungpatientswithintexas |