An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation
Abstract Objective Understanding the regulation and function of plant genes is essential for addressing the challenges faced by modern agriculture. Plant transformation, in conjunction with fluorescence microscopy, offers a powerful approach to investigate the dynamic behavior of plant genes and the...
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| Language: | English |
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BMC
2025-04-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-025-07231-1 |
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| author | Jeffrey R. Allen Michael Flanagan Sunita Pathak Samantha K. Emenecker Ryan J. Emenecker Lucia C. Strader |
| author_facet | Jeffrey R. Allen Michael Flanagan Sunita Pathak Samantha K. Emenecker Ryan J. Emenecker Lucia C. Strader |
| author_sort | Jeffrey R. Allen |
| collection | DOAJ |
| description | Abstract Objective Understanding the regulation and function of plant genes is essential for addressing the challenges faced by modern agriculture. Plant transformation, in conjunction with fluorescence microscopy, offers a powerful approach to investigate the dynamic behavior of plant genes and the proteins they encode. We previously developed a set of Gateway-compatible tissue-specific plant transformation vectors. In this paper we aim to expand the toolkit of vectors available for Agrobacterium-mediated plant transformation and protoplast transfection. Results Here, we introduce new Agrobacterium-mediated plant transformation vectors by introducing additional fluorophores to create the pJRA vector series. Additionally, we introduce the pLCS series of vectors, a new set of modular Gateway- and Gibson assembly-compatible vectors designed for protoplast transfection. All described vectors are available from Addgene to serve as a resource for the plant research community. |
| format | Article |
| id | doaj-art-fee3482d83fb4877a82ba9c1d344eb7b |
| institution | OA Journals |
| issn | 1756-0500 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-fee3482d83fb4877a82ba9c1d344eb7b2025-08-20T02:11:58ZengBMCBMC Research Notes1756-05002025-04-011811810.1186/s13104-025-07231-1An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformationJeffrey R. Allen0Michael Flanagan1Sunita Pathak2Samantha K. Emenecker3Ryan J. Emenecker4Lucia C. Strader5Department of Biology, Duke UniversityDepartment of Biology, Duke UniversityDepartment of Biology, Duke UniversityDepartment of Biology, Washington University in St. LouisDepartment of Biology, Washington University in St. LouisDepartment of Biology, Duke UniversityAbstract Objective Understanding the regulation and function of plant genes is essential for addressing the challenges faced by modern agriculture. Plant transformation, in conjunction with fluorescence microscopy, offers a powerful approach to investigate the dynamic behavior of plant genes and the proteins they encode. We previously developed a set of Gateway-compatible tissue-specific plant transformation vectors. In this paper we aim to expand the toolkit of vectors available for Agrobacterium-mediated plant transformation and protoplast transfection. Results Here, we introduce new Agrobacterium-mediated plant transformation vectors by introducing additional fluorophores to create the pJRA vector series. Additionally, we introduce the pLCS series of vectors, a new set of modular Gateway- and Gibson assembly-compatible vectors designed for protoplast transfection. All described vectors are available from Addgene to serve as a resource for the plant research community.https://doi.org/10.1186/s13104-025-07231-1Protoplast transfectionAgrobacterium transformationTransgenic plantPlant transformationTransient expressionLive cell imaging |
| spellingShingle | Jeffrey R. Allen Michael Flanagan Sunita Pathak Samantha K. Emenecker Ryan J. Emenecker Lucia C. Strader An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation BMC Research Notes Protoplast transfection Agrobacterium transformation Transgenic plant Plant transformation Transient expression Live cell imaging |
| title | An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation |
| title_full | An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation |
| title_fullStr | An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation |
| title_full_unstemmed | An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation |
| title_short | An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation |
| title_sort | improved toolkit of gateway and gibson assembly compatible vectors for protoplast transfection and agrobacterium mediated plant transformation |
| topic | Protoplast transfection Agrobacterium transformation Transgenic plant Plant transformation Transient expression Live cell imaging |
| url | https://doi.org/10.1186/s13104-025-07231-1 |
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