Flammulina velutipes polysaccharide-iron(III) complex used to treat iron deficiency anemia after being absorbed via GLUT2 and SGLT1 transporters
Iron deficiency anemia (IDA) is a common nutritional problem, but traditional iron supplements cause many adverse reactions. Thus, the development of a novel iron supplement might be significant for the treatment of IDA. This study aimed to study the transport mechanism of Flammulina velutipes polys...
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000472 |
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| author | Chenying Shi Chen Cheng Xiaotong Lin Yanfang Qian Yufeng Du Guitang Chen |
| author_facet | Chenying Shi Chen Cheng Xiaotong Lin Yanfang Qian Yufeng Du Guitang Chen |
| author_sort | Chenying Shi |
| collection | DOAJ |
| description | Iron deficiency anemia (IDA) is a common nutritional problem, but traditional iron supplements cause many adverse reactions. Thus, the development of a novel iron supplement might be significant for the treatment of IDA. This study aimed to study the transport mechanism of Flammulina velutipes polysaccharide-iron complex (FVP1-Fe(III)) in Caco-2 cells and the therapeutic effect on IDA rats, as well as the influence on gut microbiota in vivo. These results showed that in vitro, the uptake of FVP1-Fe(III) was mediated by sodium-dependent glucose transporter-1 (SGLT1) and facilitated glucose transporter-2 (GLUT2) and GLUT2 played a dominant function. The multidrug resistance-associated protein-2 (MRP-2) was involved in the efflux of FVP1-Fe(III) across the Caco-2 cells. In vivo, FVP1-Fe(III) had a better restorative effect on blood parameters and iron status indicators in rats with IDA as compared with FeSO4 and exerted this effect by downregulating the expression of hepcidin. FVP1-Fe(III) could also regulate gut microbiota dysbiosis in iron deficiency rats by returning the relative abundance of gut microbiota to the normal level. Besides, as a dietary factor, vitamin C (vit C) could enhance the therapeutic effect of FVP1-Fe(III). These present findings showed that FVP1-Fe(III) could be exploited as a novel iron supplement to treat IDA. |
| format | Article |
| id | doaj-art-fecefbda16cb4e3a949be5e24e7ae65a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-fecefbda16cb4e3a949be5e24e7ae65a2025-02-03T00:27:37ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112518281840Flammulina velutipes polysaccharide-iron(III) complex used to treat iron deficiency anemia after being absorbed via GLUT2 and SGLT1 transportersChenying Shi0Chen Cheng1Xiaotong Lin2Yanfang Qian3Yufeng Du4Guitang Chen5Department of Food Nutrition and Safety/National R&D Center for Chinese Herbal, Medicine Processing, China Pharmaceutical University, Nanjing 211198, ChinaDepartment of Food Nutrition and Safety/National R&D Center for Chinese Herbal, Medicine Processing, China Pharmaceutical University, Nanjing 211198, ChinaDepartment of Food Nutrition and Safety/National R&D Center for Chinese Herbal, Medicine Processing, China Pharmaceutical University, Nanjing 211198, ChinaDepartment of Food Nutrition and Safety/National R&D Center for Chinese Herbal, Medicine Processing, China Pharmaceutical University, Nanjing 211198, ChinaCollege of Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Corresponding authors.Department of Food Nutrition and Safety/National R&D Center for Chinese Herbal, Medicine Processing, China Pharmaceutical University, Nanjing 211198, China; Corresponding authors.Iron deficiency anemia (IDA) is a common nutritional problem, but traditional iron supplements cause many adverse reactions. Thus, the development of a novel iron supplement might be significant for the treatment of IDA. This study aimed to study the transport mechanism of Flammulina velutipes polysaccharide-iron complex (FVP1-Fe(III)) in Caco-2 cells and the therapeutic effect on IDA rats, as well as the influence on gut microbiota in vivo. These results showed that in vitro, the uptake of FVP1-Fe(III) was mediated by sodium-dependent glucose transporter-1 (SGLT1) and facilitated glucose transporter-2 (GLUT2) and GLUT2 played a dominant function. The multidrug resistance-associated protein-2 (MRP-2) was involved in the efflux of FVP1-Fe(III) across the Caco-2 cells. In vivo, FVP1-Fe(III) had a better restorative effect on blood parameters and iron status indicators in rats with IDA as compared with FeSO4 and exerted this effect by downregulating the expression of hepcidin. FVP1-Fe(III) could also regulate gut microbiota dysbiosis in iron deficiency rats by returning the relative abundance of gut microbiota to the normal level. Besides, as a dietary factor, vitamin C (vit C) could enhance the therapeutic effect of FVP1-Fe(III). These present findings showed that FVP1-Fe(III) could be exploited as a novel iron supplement to treat IDA.http://www.sciencedirect.com/science/article/pii/S2213453023000472Flammulina velutipes polysaccharideIron deficiency anemiaIron supplementCaco-2 cell modelGut microbiota |
| spellingShingle | Chenying Shi Chen Cheng Xiaotong Lin Yanfang Qian Yufeng Du Guitang Chen Flammulina velutipes polysaccharide-iron(III) complex used to treat iron deficiency anemia after being absorbed via GLUT2 and SGLT1 transporters Food Science and Human Wellness Flammulina velutipes polysaccharide Iron deficiency anemia Iron supplement Caco-2 cell model Gut microbiota |
| title | Flammulina velutipes polysaccharide-iron(III) complex used to treat iron deficiency anemia after being absorbed via GLUT2 and SGLT1 transporters |
| title_full | Flammulina velutipes polysaccharide-iron(III) complex used to treat iron deficiency anemia after being absorbed via GLUT2 and SGLT1 transporters |
| title_fullStr | Flammulina velutipes polysaccharide-iron(III) complex used to treat iron deficiency anemia after being absorbed via GLUT2 and SGLT1 transporters |
| title_full_unstemmed | Flammulina velutipes polysaccharide-iron(III) complex used to treat iron deficiency anemia after being absorbed via GLUT2 and SGLT1 transporters |
| title_short | Flammulina velutipes polysaccharide-iron(III) complex used to treat iron deficiency anemia after being absorbed via GLUT2 and SGLT1 transporters |
| title_sort | flammulina velutipes polysaccharide iron iii complex used to treat iron deficiency anemia after being absorbed via glut2 and sglt1 transporters |
| topic | Flammulina velutipes polysaccharide Iron deficiency anemia Iron supplement Caco-2 cell model Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000472 |
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