Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags
Here, we report a SERS based VFA using PNC as a sensing substrate for highly sensitive multiplex mycotoxins detection. The PNC was fabricated by filtration-based self-assembled monodisperse SiO2 NPs on a filter membrane as a template, and the obtained PNC had an ordered complementary inverse opal st...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S259015752401040X |
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| author | Ruipeng Chen Jingfang Hu Hui Wang Cheng Li Haiqi Kang Yuxuan Chen Liang Yang Xiangfang Tang Benhai Xiong Xiangwei Zhao |
| author_facet | Ruipeng Chen Jingfang Hu Hui Wang Cheng Li Haiqi Kang Yuxuan Chen Liang Yang Xiangfang Tang Benhai Xiong Xiangwei Zhao |
| author_sort | Ruipeng Chen |
| collection | DOAJ |
| description | Here, we report a SERS based VFA using PNC as a sensing substrate for highly sensitive multiplex mycotoxins detection. The PNC was fabricated by filtration-based self-assembled monodisperse SiO2 NPs on a filter membrane as a template, and the obtained PNC had an ordered complementary inverse opal structure. In parallel, three kinds of Raman dyes encoding Au@NBAAg, Au@4-MBAAg and Au@DNTBAg SERS nanotags were synthesized for the detection of OTA, AFB1 and ZON. In the immunoassay, because of the slow-photon effect of the PNC substrate, which facilitates the light coupling with SERS nanotags, the SERS signal was highly enhanced. The LODs of 8.2, 13.7 and 47.6 fg mL−1 were achieved for simultaneous detection of OTA, AFB1 and ZON, respectively, which were lower than the tolerable cutoff values recommended by the EC. Therefore, this proposed PNC based SERS VFA is expected to be a powerful method for multiplex mycotoxins detection. |
| format | Article |
| id | doaj-art-fec6a9294e1b419684d2aed6c096ebdc |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-fec6a9294e1b419684d2aed6c096ebdc2025-02-12T05:32:14ZengElsevierFood Chemistry: X2590-15752025-01-0125102152Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotagsRuipeng Chen0Jingfang Hu1Hui Wang2Cheng Li3Haiqi Kang4Yuxuan Chen5Liang Yang6Xiangfang Tang7Benhai Xiong8Xiangwei Zhao9State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaBeijing key Laboratory of Sensor, Beijing Information Science & Technology University, Beijing 100101, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaCollege of Economics and Management, Beijing University of Agriculture, Beijing 102206, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Corresponding authors.State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Corresponding authors.State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China; Southeast University Shenzhen Research Institute, Shenzhen 518000, China; Corresponding author at: State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.Here, we report a SERS based VFA using PNC as a sensing substrate for highly sensitive multiplex mycotoxins detection. The PNC was fabricated by filtration-based self-assembled monodisperse SiO2 NPs on a filter membrane as a template, and the obtained PNC had an ordered complementary inverse opal structure. In parallel, three kinds of Raman dyes encoding Au@NBAAg, Au@4-MBAAg and Au@DNTBAg SERS nanotags were synthesized for the detection of OTA, AFB1 and ZON. In the immunoassay, because of the slow-photon effect of the PNC substrate, which facilitates the light coupling with SERS nanotags, the SERS signal was highly enhanced. The LODs of 8.2, 13.7 and 47.6 fg mL−1 were achieved for simultaneous detection of OTA, AFB1 and ZON, respectively, which were lower than the tolerable cutoff values recommended by the EC. Therefore, this proposed PNC based SERS VFA is expected to be a powerful method for multiplex mycotoxins detection.http://www.sciencedirect.com/science/article/pii/S259015752401040XSurface-enhanced Raman scatteringVertical flow assayPhotonic nitrocellulose (PNC)Slow-photon effectMultiplex mycotoxin detection |
| spellingShingle | Ruipeng Chen Jingfang Hu Hui Wang Cheng Li Haiqi Kang Yuxuan Chen Liang Yang Xiangfang Tang Benhai Xiong Xiangwei Zhao Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags Food Chemistry: X Surface-enhanced Raman scattering Vertical flow assay Photonic nitrocellulose (PNC) Slow-photon effect Multiplex mycotoxin detection |
| title | Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags |
| title_full | Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags |
| title_fullStr | Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags |
| title_full_unstemmed | Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags |
| title_short | Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags |
| title_sort | vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and sers nanotags |
| topic | Surface-enhanced Raman scattering Vertical flow assay Photonic nitrocellulose (PNC) Slow-photon effect Multiplex mycotoxin detection |
| url | http://www.sciencedirect.com/science/article/pii/S259015752401040X |
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