Mutagenesis of Ganoderma lucidum protoplasts for high rate of biomass production and Se<sup>+4</sup>-rich isolates

Protoplasts were isolated from Ganoderma lucidum CJXN-9802. Factors including enzymes, hypha age, osmotants, and temperature were studied with respect their influence on protoplast production. Rapid growth mutants were obtained through screening from UV-light irradiated protoplasts. One of the mutan...

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Bibliographic Details
Main Authors: LIU Shi-wang, GUO Ze-jian, KAN Yun-chao, LI Nan-yi, LI Jin
Format: Article
Language:English
Published: Zhejiang University Press 2003-01-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2003.01.0044
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Summary:Protoplasts were isolated from Ganoderma lucidum CJXN-9802. Factors including enzymes, hypha age, osmotants, and temperature were studied with respect their influence on protoplast production. Rapid growth mutants were obtained through screening from UV-light irradiated protoplasts. One of the mutants CJXN-980222 showed increase in biomass 9.4% and 14.3% increase in Se<sup>+4</sup> content, compared with the original strain.
ISSN:1008-9209
2097-5155