Phylogenomic Analysis of Dichrocephala benthamii and Comparative Analysis within Tribe Astereae (Asteraceae)

Abstract Dichrocephala benthamii C. B. Clarke has long been used as traditional Chinese medicine. However, the chloroplast (cp) genome of D. benthamii is poorly understood so far. In this study, we sequenced and analyzed the cp genome of D. benthamii. The results showed that the cp genome is 152,350...

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Main Authors: Hui Chen, Tingyu Li, Xinyu Chen, Xinyi Zheng, Tianmeng Qu, Bo Li, Zhixi Fu
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2024-10-01
Series:Genetics and Molecular Biology
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572024000700801&lng=en&tlng=en
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author Hui Chen
Tingyu Li
Xinyu Chen
Xinyi Zheng
Tianmeng Qu
Bo Li
Zhixi Fu
author_facet Hui Chen
Tingyu Li
Xinyu Chen
Xinyi Zheng
Tianmeng Qu
Bo Li
Zhixi Fu
author_sort Hui Chen
collection DOAJ
description Abstract Dichrocephala benthamii C. B. Clarke has long been used as traditional Chinese medicine. However, the chloroplast (cp) genome of D. benthamii is poorly understood so far. In this study, we sequenced and analyzed the cp genome of D. benthamii. The results showed that the cp genome is 152,350 bp in length, with a pair of inverted repeat regions (IRa and IRb, each 24,982 bp), a large single-copy (LSC) region comprising 84,136 bp, and a small single-copy (SSC) region comprising 18,250 bp. The GC content of the cp genome was 37.3%. A total of 134 genes were identified, including 87 protein-coding genes (CDS), 38 tRNA genes, 8 rRNA genes, and 1 pseudogene (ycf1). Expansion or contraction of IR regions were detected in D. benthamii and other species of the tribe Astereae. Additionally, our analyses showed the types of sequence repeats and the highly variable regions discovered by analyzing the border regions, sequence divergence, and hot spots. The phylogenetic analysis revealed D. benthamii is the basal group of Astereae. The results of this study will be a significant contribution to the genetics and species identification related to D. benthamii.
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spelling doaj-art-fe9f0761201543769a5c13fe4cf89a702025-08-20T02:09:27ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852024-10-0147410.1590/1678-4685-gmb-2023-0340Phylogenomic Analysis of Dichrocephala benthamii and Comparative Analysis within Tribe Astereae (Asteraceae)Hui ChenTingyu Lihttps://orcid.org/0009-0005-6873-2114Xinyu ChenXinyi ZhengTianmeng QuBo LiZhixi Fuhttps://orcid.org/0000-0002-2789-6287Abstract Dichrocephala benthamii C. B. Clarke has long been used as traditional Chinese medicine. However, the chloroplast (cp) genome of D. benthamii is poorly understood so far. In this study, we sequenced and analyzed the cp genome of D. benthamii. The results showed that the cp genome is 152,350 bp in length, with a pair of inverted repeat regions (IRa and IRb, each 24,982 bp), a large single-copy (LSC) region comprising 84,136 bp, and a small single-copy (SSC) region comprising 18,250 bp. The GC content of the cp genome was 37.3%. A total of 134 genes were identified, including 87 protein-coding genes (CDS), 38 tRNA genes, 8 rRNA genes, and 1 pseudogene (ycf1). Expansion or contraction of IR regions were detected in D. benthamii and other species of the tribe Astereae. Additionally, our analyses showed the types of sequence repeats and the highly variable regions discovered by analyzing the border regions, sequence divergence, and hot spots. The phylogenetic analysis revealed D. benthamii is the basal group of Astereae. The results of this study will be a significant contribution to the genetics and species identification related to D. benthamii.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572024000700801&lng=en&tlng=enDichrocephala benthamiichloroplast genomephylogenetic analysiscomparative analysis
spellingShingle Hui Chen
Tingyu Li
Xinyu Chen
Xinyi Zheng
Tianmeng Qu
Bo Li
Zhixi Fu
Phylogenomic Analysis of Dichrocephala benthamii and Comparative Analysis within Tribe Astereae (Asteraceae)
Genetics and Molecular Biology
Dichrocephala benthamii
chloroplast genome
phylogenetic analysis
comparative analysis
title Phylogenomic Analysis of Dichrocephala benthamii and Comparative Analysis within Tribe Astereae (Asteraceae)
title_full Phylogenomic Analysis of Dichrocephala benthamii and Comparative Analysis within Tribe Astereae (Asteraceae)
title_fullStr Phylogenomic Analysis of Dichrocephala benthamii and Comparative Analysis within Tribe Astereae (Asteraceae)
title_full_unstemmed Phylogenomic Analysis of Dichrocephala benthamii and Comparative Analysis within Tribe Astereae (Asteraceae)
title_short Phylogenomic Analysis of Dichrocephala benthamii and Comparative Analysis within Tribe Astereae (Asteraceae)
title_sort phylogenomic analysis of dichrocephala benthamii and comparative analysis within tribe astereae asteraceae
topic Dichrocephala benthamii
chloroplast genome
phylogenetic analysis
comparative analysis
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572024000700801&lng=en&tlng=en
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