MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axis
Abstract Objective The regulatory role of the miR-618/JAK2/STAT3 axis in non-small cell lung cancer cells (NSCLC) was investigated with the objective of identifying a target for the precise treatment of patients with NSCLC. Methods Initially, differential gene expression was identified in the GEO da...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | Journal of Cardiothoracic Surgery |
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| Online Access: | https://doi.org/10.1186/s13019-024-03160-5 |
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| author | Ziyuan Chen Wei Chen Zhiqi Hong Xianqiao Wu Tianzheng Fang Yufei Sheng Shuai Fang Chengwei Zhou |
| author_facet | Ziyuan Chen Wei Chen Zhiqi Hong Xianqiao Wu Tianzheng Fang Yufei Sheng Shuai Fang Chengwei Zhou |
| author_sort | Ziyuan Chen |
| collection | DOAJ |
| description | Abstract Objective The regulatory role of the miR-618/JAK2/STAT3 axis in non-small cell lung cancer cells (NSCLC) was investigated with the objective of identifying a target for the precise treatment of patients with NSCLC. Methods Initially, differential gene expression was identified in the GEO database, followed by a comprehensive bioinformatics analysis. The tissue and cell levels of miR-618 were assessed using qRT-PCR, while the protein levels of JAK2 and STAT3 were determined through western blotting analysis. The association between miR-618 and JAK2 was scrutinized through bioinformatics analysis and dual-luciferase experiments. To evaluate cell proliferation, migration, and invasion, MTT, wound-healing, and Transwell assays were employed. Results The expression of miR-618 is decreased in NSCLC, and it targets the JAK2/STAT3 pathway to inhibit the proliferation, invasion, and migration of NSCLC. Conclusion Our study demonstrates that a novel miR-618/JAK2/STAT3 signaling axis is involved in suppressing malignancy in NSCLC and provides a promising target for NSCLC therapy. |
| format | Article |
| id | doaj-art-fe9ed84cf1c84b8d9fa4de93b7a07575 |
| institution | DOAJ |
| issn | 1749-8090 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Cardiothoracic Surgery |
| spelling | doaj-art-fe9ed84cf1c84b8d9fa4de93b7a075752025-08-20T02:39:35ZengBMCJournal of Cardiothoracic Surgery1749-80902024-12-011911910.1186/s13019-024-03160-5MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axisZiyuan Chen0Wei Chen1Zhiqi Hong2Xianqiao Wu3Tianzheng Fang4Yufei Sheng5Shuai Fang6Chengwei Zhou7Department of Thoracic Surgery, The First Affiliated Hospital of Ningbo UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Ningbo UniversityAbstract Objective The regulatory role of the miR-618/JAK2/STAT3 axis in non-small cell lung cancer cells (NSCLC) was investigated with the objective of identifying a target for the precise treatment of patients with NSCLC. Methods Initially, differential gene expression was identified in the GEO database, followed by a comprehensive bioinformatics analysis. The tissue and cell levels of miR-618 were assessed using qRT-PCR, while the protein levels of JAK2 and STAT3 were determined through western blotting analysis. The association between miR-618 and JAK2 was scrutinized through bioinformatics analysis and dual-luciferase experiments. To evaluate cell proliferation, migration, and invasion, MTT, wound-healing, and Transwell assays were employed. Results The expression of miR-618 is decreased in NSCLC, and it targets the JAK2/STAT3 pathway to inhibit the proliferation, invasion, and migration of NSCLC. Conclusion Our study demonstrates that a novel miR-618/JAK2/STAT3 signaling axis is involved in suppressing malignancy in NSCLC and provides a promising target for NSCLC therapy.https://doi.org/10.1186/s13019-024-03160-5Non-small cell lung cancermiRNAJAK2miR-618STAT3 |
| spellingShingle | Ziyuan Chen Wei Chen Zhiqi Hong Xianqiao Wu Tianzheng Fang Yufei Sheng Shuai Fang Chengwei Zhou MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axis Journal of Cardiothoracic Surgery Non-small cell lung cancer miRNA JAK2 miR-618 STAT3 |
| title | MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axis |
| title_full | MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axis |
| title_fullStr | MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axis |
| title_full_unstemmed | MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axis |
| title_short | MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axis |
| title_sort | mir 618 suppresses the proliferation invasion and migration of non small lung cancer via the jak2 stat3 axis |
| topic | Non-small cell lung cancer miRNA JAK2 miR-618 STAT3 |
| url | https://doi.org/10.1186/s13019-024-03160-5 |
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