Antinociceptive and Antioxidant Activity of Zanthoxylum budrunga Wall (Rutaceae) Seeds
Different parts of the medicinal plant Zanthoxylum budrunga Wall enjoy a variety of uses in ethnobotanical practice in Bangladesh. In the present study, a number of phytochemical and pharmacological investigations were done on the ethanol extract of Z. budrunga seeds (ZBSE) to evaluate its antinocic...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/869537 |
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| author | Md. Khirul Islam Nripendra Nath Biswas Sanjib Saha Hemayet Hossain Ismet Ara Jahan Tanzir Ahmed Khan Khalijah Awang Jamil A. Shilpi |
| author_facet | Md. Khirul Islam Nripendra Nath Biswas Sanjib Saha Hemayet Hossain Ismet Ara Jahan Tanzir Ahmed Khan Khalijah Awang Jamil A. Shilpi |
| author_sort | Md. Khirul Islam |
| collection | DOAJ |
| description | Different parts of the medicinal plant Zanthoxylum budrunga Wall enjoy a variety of uses in ethnobotanical practice in Bangladesh. In the present study, a number of phytochemical and pharmacological investigations were done on the ethanol extract of Z. budrunga seeds (ZBSE) to evaluate its antinociceptive and antioxidant potential. ZBSE was also subjected to HPLC analysis to detect the presence of some common antioxidants. In acetic acid induced writhing test in mice, ZBSE showed 65.28 and 74.30% inhibition of writhing at the doses of 250 and 500 mg/kg and the results were statistically significant (P<0.001). In hot-plate test, ZBSE raised the pain threshold significantly (P<0.001) throughout the entire observation period. In DPPH scavenging assay, the IC50 of ZBSE was observed at 82.60 μg/mL. The phenolic content was found to be 338.77 mg GAE/100 g of dried plant material. In reducing power assay, ZBSE showed a concentration dependent reducing ability. HPLC analysis indicated the presence of caffeic acid with a concentration of 75.45 mg/100 g ZBSE. Present investigation supported the use of Zanthoxylum budrunga seed in traditional medicine for pain management. Constituents including caffeic acid and other phenolics might have some role in the observed activity. |
| format | Article |
| id | doaj-art-fe9c8a5b40ae403190040f33b646e82b |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-fe9c8a5b40ae403190040f33b646e82b2025-02-03T01:29:56ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/869537869537Antinociceptive and Antioxidant Activity of Zanthoxylum budrunga Wall (Rutaceae) SeedsMd. Khirul Islam0Nripendra Nath Biswas1Sanjib Saha2Hemayet Hossain3Ismet Ara Jahan4Tanzir Ahmed Khan5Khalijah Awang6Jamil A. Shilpi7Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, BangladeshSchool of Chemistry, University of New South Wales, Sydney, NSW 2052, AustraliaPharmacy Discipline, Life Science School, Khulna University, Khulna 9208, BangladeshChemical Research Division, BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, BangladeshChemical Research Division, BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, BangladeshChemical Research Division, BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, BangladeshDepartment of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaCentre for Natural Products and Drug, University of Malaya, 50603 Kuala Lumpur, MalaysiaDifferent parts of the medicinal plant Zanthoxylum budrunga Wall enjoy a variety of uses in ethnobotanical practice in Bangladesh. In the present study, a number of phytochemical and pharmacological investigations were done on the ethanol extract of Z. budrunga seeds (ZBSE) to evaluate its antinociceptive and antioxidant potential. ZBSE was also subjected to HPLC analysis to detect the presence of some common antioxidants. In acetic acid induced writhing test in mice, ZBSE showed 65.28 and 74.30% inhibition of writhing at the doses of 250 and 500 mg/kg and the results were statistically significant (P<0.001). In hot-plate test, ZBSE raised the pain threshold significantly (P<0.001) throughout the entire observation period. In DPPH scavenging assay, the IC50 of ZBSE was observed at 82.60 μg/mL. The phenolic content was found to be 338.77 mg GAE/100 g of dried plant material. In reducing power assay, ZBSE showed a concentration dependent reducing ability. HPLC analysis indicated the presence of caffeic acid with a concentration of 75.45 mg/100 g ZBSE. Present investigation supported the use of Zanthoxylum budrunga seed in traditional medicine for pain management. Constituents including caffeic acid and other phenolics might have some role in the observed activity.http://dx.doi.org/10.1155/2014/869537 |
| spellingShingle | Md. Khirul Islam Nripendra Nath Biswas Sanjib Saha Hemayet Hossain Ismet Ara Jahan Tanzir Ahmed Khan Khalijah Awang Jamil A. Shilpi Antinociceptive and Antioxidant Activity of Zanthoxylum budrunga Wall (Rutaceae) Seeds The Scientific World Journal |
| title | Antinociceptive and Antioxidant Activity of Zanthoxylum budrunga Wall (Rutaceae) Seeds |
| title_full | Antinociceptive and Antioxidant Activity of Zanthoxylum budrunga Wall (Rutaceae) Seeds |
| title_fullStr | Antinociceptive and Antioxidant Activity of Zanthoxylum budrunga Wall (Rutaceae) Seeds |
| title_full_unstemmed | Antinociceptive and Antioxidant Activity of Zanthoxylum budrunga Wall (Rutaceae) Seeds |
| title_short | Antinociceptive and Antioxidant Activity of Zanthoxylum budrunga Wall (Rutaceae) Seeds |
| title_sort | antinociceptive and antioxidant activity of zanthoxylum budrunga wall rutaceae seeds |
| url | http://dx.doi.org/10.1155/2014/869537 |
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