Isolation, identification and optimization of fermentation conditions against Sclerotinia sclerotiorum strains in high salt Doenjang
With the global warming, the cultivation and storage of northern Chinese cabbage has been infected by Sclerotium bacteria, resulting in the whole plant rot. In this study, in order to develop a bio-inhibitory agent with antibacterial effect and no toxicity and no residue, three strains with high inh...
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| Format: | Article |
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Tsinghua University Press
2021-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000185 |
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| author | Xian Zhang Hongmei Li Xiaoyu Kang Seongil Lim Fanzhu Li |
| author_facet | Xian Zhang Hongmei Li Xiaoyu Kang Seongil Lim Fanzhu Li |
| author_sort | Xian Zhang |
| collection | DOAJ |
| description | With the global warming, the cultivation and storage of northern Chinese cabbage has been infected by Sclerotium bacteria, resulting in the whole plant rot. In this study, in order to develop a bio-inhibitory agent with antibacterial effect and no toxicity and no residue, three strains with high inhibition rate against Sclerotinia sclerotiorum were screened from 69 samples of Doenjang collected in different regions. By observing culture characteristics and physiological and biochemical tests, three strains were identified through 16S rDNA sequence and phylogenetic tree. The results showed that SP-6 was Bacillus amyloliquefaciens, SP-13 was Bacillus methylotrophicus, and SP-14 was Bacillus subtilis. Meanwhile, the inhibition rate of the aseptic fermentation filtrate of three strains against S. sclerotiorum was determined and the fermentation conditions of SP-14 strain was optimized. Results showed that all of three fermentation filtrates inhibited S. sclerotiorum. Data showed that the best conditions for fermentation for the antibacterial substances of SP-14 strain is as following: 1% maltose, 1% ammonium oxalate or beef extract, 1% ferrous sulfate, fermentation at 32 °C for 36 h, initial pH 8–9 and the inoculation amount 5%. The living bacteriostatic test showed that SP-14 strain had good inhibitory effect on S. sclerotiorum and the inhibitory effect was more significant when the bacteria concentration was 1.2 × 1011 CFU/mL. |
| format | Article |
| id | doaj-art-fe8f52612d4f460bb824ddfce16f5ac1 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2021-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-fe8f52612d4f460bb824ddfce16f5ac12025-02-03T06:51:00ZengTsinghua University PressFood Science and Human Wellness2213-45302021-03-01102205213Isolation, identification and optimization of fermentation conditions against Sclerotinia sclerotiorum strains in high salt DoenjangXian Zhang0Hongmei Li1Xiaoyu Kang2Seongil Lim3Fanzhu Li4Agricultural College, Yanbian University, Yanji, Jilin, ChinaAgricultural College, Yanbian University, Yanji, Jilin, ChinaAgricultural College, Yanbian University, Yanji, Jilin, ChinaFermentation and Functionality Research Group, Korea Food Research Institute, KoreaAgricultural College, Yanbian University, Yanji, Jilin, China; Corresponding author. Department of food science, Agricultural college, Yanbian University, Yanji, Jilin Province 133000, China.With the global warming, the cultivation and storage of northern Chinese cabbage has been infected by Sclerotium bacteria, resulting in the whole plant rot. In this study, in order to develop a bio-inhibitory agent with antibacterial effect and no toxicity and no residue, three strains with high inhibition rate against Sclerotinia sclerotiorum were screened from 69 samples of Doenjang collected in different regions. By observing culture characteristics and physiological and biochemical tests, three strains were identified through 16S rDNA sequence and phylogenetic tree. The results showed that SP-6 was Bacillus amyloliquefaciens, SP-13 was Bacillus methylotrophicus, and SP-14 was Bacillus subtilis. Meanwhile, the inhibition rate of the aseptic fermentation filtrate of three strains against S. sclerotiorum was determined and the fermentation conditions of SP-14 strain was optimized. Results showed that all of three fermentation filtrates inhibited S. sclerotiorum. Data showed that the best conditions for fermentation for the antibacterial substances of SP-14 strain is as following: 1% maltose, 1% ammonium oxalate or beef extract, 1% ferrous sulfate, fermentation at 32 °C for 36 h, initial pH 8–9 and the inoculation amount 5%. The living bacteriostatic test showed that SP-14 strain had good inhibitory effect on S. sclerotiorum and the inhibitory effect was more significant when the bacteria concentration was 1.2 × 1011 CFU/mL.http://www.sciencedirect.com/science/article/pii/S2213453021000185DoenjangBacillusSclerotinia sclerotiorumAntagonism |
| spellingShingle | Xian Zhang Hongmei Li Xiaoyu Kang Seongil Lim Fanzhu Li Isolation, identification and optimization of fermentation conditions against Sclerotinia sclerotiorum strains in high salt Doenjang Food Science and Human Wellness Doenjang Bacillus Sclerotinia sclerotiorum Antagonism |
| title | Isolation, identification and optimization of fermentation conditions against Sclerotinia sclerotiorum strains in high salt Doenjang |
| title_full | Isolation, identification and optimization of fermentation conditions against Sclerotinia sclerotiorum strains in high salt Doenjang |
| title_fullStr | Isolation, identification and optimization of fermentation conditions against Sclerotinia sclerotiorum strains in high salt Doenjang |
| title_full_unstemmed | Isolation, identification and optimization of fermentation conditions against Sclerotinia sclerotiorum strains in high salt Doenjang |
| title_short | Isolation, identification and optimization of fermentation conditions against Sclerotinia sclerotiorum strains in high salt Doenjang |
| title_sort | isolation identification and optimization of fermentation conditions against sclerotinia sclerotiorum strains in high salt doenjang |
| topic | Doenjang Bacillus Sclerotinia sclerotiorum Antagonism |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000185 |
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