Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf Extract

Ocimum sanctum (OS) Linn, is a medicinal herb that has been used in traditional medicine science ancient time. It contains an array of complex and diverse biologically active phytochemical constituents that can act as a reducing and stabilizing agent for synthesizing metal nanoparticles (NPs). Herei...

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Main Authors: Malik Ahmad Al-Natour, Mohammad Faiyaz Anwar, Shah Alam Khan, Mohammad Changez
Format: Article
Language:English
Published: Tamkang University Press 2025-03-01
Series:Journal of Applied Science and Engineering
Subjects:
Online Access:http://jase.tku.edu.tw/articles/jase-202510-28-10-0006
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author Malik Ahmad Al-Natour
Mohammad Faiyaz Anwar
Shah Alam Khan
Mohammad Changez
author_facet Malik Ahmad Al-Natour
Mohammad Faiyaz Anwar
Shah Alam Khan
Mohammad Changez
author_sort Malik Ahmad Al-Natour
collection DOAJ
description Ocimum sanctum (OS) Linn, is a medicinal herb that has been used in traditional medicine science ancient time. It contains an array of complex and diverse biologically active phytochemical constituents that can act as a reducing and stabilizing agent for synthesizing metal nanoparticles (NPs). Herein, OS aqueous leaf extract was used to synthesize eco-friendly round and rod-shaped Ag NPs. The synthesized spherical and rod-shaped Ag NPs were analyzed by indirect methods by analysis of plasma band (UV-vis spectra) and thin films X-ray diffraction (XRD) peaks. Whereas, for direct methods, morphologies of synthesized Ag NPs were recoded using HR-TEM. Ag NPs demonstrated dose-dependent antibacterial efficacy against bacteria ( S. aureus). The MIC and MBC values of spherical Ag NPs against 106CFU/mL of S. aureus were found to be 2.74 and 3.80µ g/mL, respectively. However, MIC and MBC values of Ag nano-rod were observed at 7.20µ g/mL and 13µ g/mL, respectively. SEM micrographs indicate that morphologies of spherical shape and nano-rod Ag NPs treated S. aureus showed breakdown, merge, and elongated morphologies with the presence of Ag NPs (nano-rod) inside of the S. aureus.
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issn 2708-9967
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publishDate 2025-03-01
publisher Tamkang University Press
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series Journal of Applied Science and Engineering
spelling doaj-art-fe7e301a34b148999eaf2f917b7f15962025-08-20T03:16:06ZengTamkang University PressJournal of Applied Science and Engineering2708-99672708-99752025-03-0128101929193710.6180/jase.202510_28(10).0006Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf ExtractMalik Ahmad Al-Natour0Mohammad Faiyaz Anwar1Shah Alam Khan2Mohammad Changez3College of Health Sciences, University of Buraimi, OmanDepartment of Microbiology, All India Institute of Medical Sciences, New Delhi, IndiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, National University of Science and Technology, Muscat, OmanResearch, Innovation, and Entrepreneurship Unit, University of Buraimi, OmanOcimum sanctum (OS) Linn, is a medicinal herb that has been used in traditional medicine science ancient time. It contains an array of complex and diverse biologically active phytochemical constituents that can act as a reducing and stabilizing agent for synthesizing metal nanoparticles (NPs). Herein, OS aqueous leaf extract was used to synthesize eco-friendly round and rod-shaped Ag NPs. The synthesized spherical and rod-shaped Ag NPs were analyzed by indirect methods by analysis of plasma band (UV-vis spectra) and thin films X-ray diffraction (XRD) peaks. Whereas, for direct methods, morphologies of synthesized Ag NPs were recoded using HR-TEM. Ag NPs demonstrated dose-dependent antibacterial efficacy against bacteria ( S. aureus). The MIC and MBC values of spherical Ag NPs against 106CFU/mL of S. aureus were found to be 2.74 and 3.80µ g/mL, respectively. However, MIC and MBC values of Ag nano-rod were observed at 7.20µ g/mL and 13µ g/mL, respectively. SEM micrographs indicate that morphologies of spherical shape and nano-rod Ag NPs treated S. aureus showed breakdown, merge, and elongated morphologies with the presence of Ag NPs (nano-rod) inside of the S. aureus.http://jase.tku.edu.tw/articles/jase-202510-28-10-0006green chemistryocimum sanctumstaphylococcus aureussilver nanoparticlestransmission electron microscopexrd
spellingShingle Malik Ahmad Al-Natour
Mohammad Faiyaz Anwar
Shah Alam Khan
Mohammad Changez
Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf Extract
Journal of Applied Science and Engineering
green chemistry
ocimum sanctum
staphylococcus aureus
silver nanoparticles
transmission electron microscope
xrd
title Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf Extract
title_full Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf Extract
title_fullStr Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf Extract
title_full_unstemmed Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf Extract
title_short Characterization and Antimicrobial Activity of Silverrods Nano: Synthesized in Green Ocimum sanctum Leaf Extract
title_sort characterization and antimicrobial activity of silverrods nano synthesized in green ocimum sanctum leaf extract
topic green chemistry
ocimum sanctum
staphylococcus aureus
silver nanoparticles
transmission electron microscope
xrd
url http://jase.tku.edu.tw/articles/jase-202510-28-10-0006
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AT mohammadfaiyazanwar characterizationandantimicrobialactivityofsilverrodsnanosynthesizedingreenocimumsanctumleafextract
AT shahalamkhan characterizationandantimicrobialactivityofsilverrodsnanosynthesizedingreenocimumsanctumleafextract
AT mohammadchangez characterizationandantimicrobialactivityofsilverrodsnanosynthesizedingreenocimumsanctumleafextract