Recent Advance in Single-Molecule Fluorescent Biosensors for Tumor Biomarker Detection
The construction of biosensors for specific, sensitive, and rapid detection of tumor biomarkers significantly contributes to biomedical research and early cancer diagnosis. However, conventional assays often involve large sample consumption and poor sensitivity, limiting their further application in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/14/11/540 |
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| author | Jie Zhang Jiawen Liu Lixue Qiao Qian Zhang Juan Hu Chun-yang Zhang |
| author_facet | Jie Zhang Jiawen Liu Lixue Qiao Qian Zhang Juan Hu Chun-yang Zhang |
| author_sort | Jie Zhang |
| collection | DOAJ |
| description | The construction of biosensors for specific, sensitive, and rapid detection of tumor biomarkers significantly contributes to biomedical research and early cancer diagnosis. However, conventional assays often involve large sample consumption and poor sensitivity, limiting their further application in real samples. In recent years, single-molecule biosensing has emerged as a robust tool for detecting and characterizing biomarkers due to its unique advantages including simplicity, low sample consumption, ultra-high sensitivity, and rapid assay time. This review summarizes the recent advances in the construction of single-molecule biosensors for the measurement of various tumor biomarkers, including DNAs, DNA modifications, RNAs, and enzymes. We give a comprehensive review about the working principles and practical applications of these single-molecule biosensors. Additionally, we discuss the challenges and limitations of current single-molecule biosensors, and highlight the future directions. |
| format | Article |
| id | doaj-art-fe74199ee6fc4585addae82ca0a7d3c9 |
| institution | OA Journals |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-fe74199ee6fc4585addae82ca0a7d3c92025-08-20T02:08:08ZengMDPI AGBiosensors2079-63742024-11-01141154010.3390/bios14110540Recent Advance in Single-Molecule Fluorescent Biosensors for Tumor Biomarker DetectionJie Zhang0Jiawen Liu1Lixue Qiao2Qian Zhang3Juan Hu4Chun-yang Zhang5School of Chemistry and Chemical Engineering, State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, ChinaCollege of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, ChinaCollege of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, ChinaSchool of Chemistry and Materials Science, Ludong University, Yantai 264025, ChinaSchool of Chemistry and Chemical Engineering, State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, ChinaSchool of Chemistry and Chemical Engineering, State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, ChinaThe construction of biosensors for specific, sensitive, and rapid detection of tumor biomarkers significantly contributes to biomedical research and early cancer diagnosis. However, conventional assays often involve large sample consumption and poor sensitivity, limiting their further application in real samples. In recent years, single-molecule biosensing has emerged as a robust tool for detecting and characterizing biomarkers due to its unique advantages including simplicity, low sample consumption, ultra-high sensitivity, and rapid assay time. This review summarizes the recent advances in the construction of single-molecule biosensors for the measurement of various tumor biomarkers, including DNAs, DNA modifications, RNAs, and enzymes. We give a comprehensive review about the working principles and practical applications of these single-molecule biosensors. Additionally, we discuss the challenges and limitations of current single-molecule biosensors, and highlight the future directions.https://www.mdpi.com/2079-6374/14/11/540biosensorbiomarkerDNA/RNA sensorenzymatic biosensorsingle-molecule biosensorfluorescence microscopy |
| spellingShingle | Jie Zhang Jiawen Liu Lixue Qiao Qian Zhang Juan Hu Chun-yang Zhang Recent Advance in Single-Molecule Fluorescent Biosensors for Tumor Biomarker Detection Biosensors biosensor biomarker DNA/RNA sensor enzymatic biosensor single-molecule biosensor fluorescence microscopy |
| title | Recent Advance in Single-Molecule Fluorescent Biosensors for Tumor Biomarker Detection |
| title_full | Recent Advance in Single-Molecule Fluorescent Biosensors for Tumor Biomarker Detection |
| title_fullStr | Recent Advance in Single-Molecule Fluorescent Biosensors for Tumor Biomarker Detection |
| title_full_unstemmed | Recent Advance in Single-Molecule Fluorescent Biosensors for Tumor Biomarker Detection |
| title_short | Recent Advance in Single-Molecule Fluorescent Biosensors for Tumor Biomarker Detection |
| title_sort | recent advance in single molecule fluorescent biosensors for tumor biomarker detection |
| topic | biosensor biomarker DNA/RNA sensor enzymatic biosensor single-molecule biosensor fluorescence microscopy |
| url | https://www.mdpi.com/2079-6374/14/11/540 |
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