Inactivation of by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinoma
This study aimed to investigate the inactivation of the parkin gene by promoter methylation and its relationship with genome instability in nasopharyngeal carcinoma. Parkin was considered as a tumor suppressor gene in various types of cancers. However, its role in nasopharyngeal carcinoma is unexplo...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-03-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317695025 |
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| author | Haifeng Ni Zhen Zhou Bo Jiang Xiaoyang Yuan Xiaolin Cao Guangwu Huang Yong Li |
| author_facet | Haifeng Ni Zhen Zhou Bo Jiang Xiaoyang Yuan Xiaolin Cao Guangwu Huang Yong Li |
| author_sort | Haifeng Ni |
| collection | DOAJ |
| description | This study aimed to investigate the inactivation of the parkin gene by promoter methylation and its relationship with genome instability in nasopharyngeal carcinoma. Parkin was considered as a tumor suppressor gene in various types of cancers. However, its role in nasopharyngeal carcinoma is unexplored. Genomic instabilities were detected in nasopharyngeal carcinoma tissues by the random amplified polymorphic DNA. The methylation-specific polymerase chain reaction, semi-quantitative reverse transcription polymerase chain reaction, and immunohistochemical analysis were used to detect methylation and mRNA and protein expression of parkin in 54 cases of nasopharyngeal carcinoma tissues and 16 cases of normal nasopharyngeal epithelia tissues, and in 5 nasopharyngeal carcinoma cell lines (CNE1, CNE2, TWO3, C666, and HONE1) and 1 normal nasopharyngeal epithelia cell line (NP69). mRNA expression of parkin in CNE1 and CNE2 was analyzed before and after methyltransferase inhibitor 5-aza-2-deoxycytidine treatment. The relationship between promoter methylation and mRNA expression, demethylation and mRNA expression, and mRNA and protein expression of the gene and clinical factors and genomic instabilities were analyzed. The mRNA and protein expression levels were significantly reduced in 54 cases of human nasopharyngeal carcinoma compared with 16 cases of normal nasopharyngeal epithelia. Parkin -methylated cases showed significantly lower mRNA and protein expression levels compared with unmethylated cases. After 5-aza-2-deoxycytidine treatment, parkin mRNA expression was restored in CNE1 and CNE2; 92.59% (50/54) of nasopharyngeal carcinoma demonstrated genomic instability. Parkin is frequently inactivated by promoter methylation, and its mRNA and protein expression correlate with lymph node metastasis and genomic instability. Parkin deficiency probably promotes tumorigenesis in nasopharyngeal carcinoma. |
| format | Article |
| id | doaj-art-fe730eb06738470da334ffd7eece66df |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-fe730eb06738470da334ffd7eece66df2025-08-20T03:22:26ZengSAGE PublishingTumor Biology1423-03802017-03-013910.1177/1010428317695025Inactivation of by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinomaHaifeng Ni0Zhen Zhou1Bo Jiang2Xiaoyang Yuan3Xiaolin Cao4Guangwu Huang5Yong Li6Department of Otolaryngology, Hangzhou First People’s Hospital, Nanjing Medical University, Hangzhou, ChinaDepartment of Otolaryngology, Hangzhou First People’s Hospital, Nanjing Medical University, Hangzhou, ChinaDepartment of Otolaryngology, Hangzhou First People’s Hospital, Nanjing Medical University, Hangzhou, ChinaDepartment of Otolaryngology, Hangzhou First People’s Hospital, Nanjing Medical University, Hangzhou, ChinaDepartment of Otolaryngology, Hangzhou First People’s Hospital, Nanjing Medical University, Hangzhou, ChinaDepartment of Otolaryngology, First Affiliated Hospital, Guangxi Medical University, Nanning, ChinaDepartment of Otolaryngology, Hangzhou First People’s Hospital, Nanjing Medical University, Hangzhou, ChinaThis study aimed to investigate the inactivation of the parkin gene by promoter methylation and its relationship with genome instability in nasopharyngeal carcinoma. Parkin was considered as a tumor suppressor gene in various types of cancers. However, its role in nasopharyngeal carcinoma is unexplored. Genomic instabilities were detected in nasopharyngeal carcinoma tissues by the random amplified polymorphic DNA. The methylation-specific polymerase chain reaction, semi-quantitative reverse transcription polymerase chain reaction, and immunohistochemical analysis were used to detect methylation and mRNA and protein expression of parkin in 54 cases of nasopharyngeal carcinoma tissues and 16 cases of normal nasopharyngeal epithelia tissues, and in 5 nasopharyngeal carcinoma cell lines (CNE1, CNE2, TWO3, C666, and HONE1) and 1 normal nasopharyngeal epithelia cell line (NP69). mRNA expression of parkin in CNE1 and CNE2 was analyzed before and after methyltransferase inhibitor 5-aza-2-deoxycytidine treatment. The relationship between promoter methylation and mRNA expression, demethylation and mRNA expression, and mRNA and protein expression of the gene and clinical factors and genomic instabilities were analyzed. The mRNA and protein expression levels were significantly reduced in 54 cases of human nasopharyngeal carcinoma compared with 16 cases of normal nasopharyngeal epithelia. Parkin -methylated cases showed significantly lower mRNA and protein expression levels compared with unmethylated cases. After 5-aza-2-deoxycytidine treatment, parkin mRNA expression was restored in CNE1 and CNE2; 92.59% (50/54) of nasopharyngeal carcinoma demonstrated genomic instability. Parkin is frequently inactivated by promoter methylation, and its mRNA and protein expression correlate with lymph node metastasis and genomic instability. Parkin deficiency probably promotes tumorigenesis in nasopharyngeal carcinoma.https://doi.org/10.1177/1010428317695025 |
| spellingShingle | Haifeng Ni Zhen Zhou Bo Jiang Xiaoyang Yuan Xiaolin Cao Guangwu Huang Yong Li Inactivation of by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinoma Tumor Biology |
| title | Inactivation of by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinoma |
| title_full | Inactivation of by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinoma |
| title_fullStr | Inactivation of by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinoma |
| title_full_unstemmed | Inactivation of by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinoma |
| title_short | Inactivation of by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinoma |
| title_sort | inactivation of by promoter methylation correlated with lymph node metastasis and genomic instability in nasopharyngeal carcinoma |
| url | https://doi.org/10.1177/1010428317695025 |
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