Clinical predictors of 3-month isoniazid rifapentine (3HP)-related adverse drug reactions (ADR) during tuberculosis preventive therapy (PAnDoRA-3HP study): an observational study protocol

Introduction Tuberculosis (TB) is the leading infectious cause of death globally. Despite WHO recommendations for TB preventive therapy (TPT), challenges persist, including incompletion of treatment and adverse drug reactions (ADRs). There is limited data on the 3-month isoniazid and rifapentine (3H...

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Main Authors: Turyahabwe Stavia, Barbara Castelnuovo, Christine Sekaggya-Wiltshire, Kelly E Dooley, Grace Banturaki, Irene Mbabazi, Ruth Mirembe Nabisere, Lucy Alinaitwe, Brian Otaalo, Florence Aber, Juliet Nampala, Rogers Owori, Josephine Bayigga, Jonathan Mayito, Eva Agnes Odongpiny Laker, Moorine Penninah Sekadde, Jotam Pasipanodya, Stella Zawedde‐Muyanja
Format: Article
Language:English
Published: BMJ Publishing Group 2024-12-01
Series:BMJ Open
Online Access:https://bmjopen.bmj.com/content/14/12/e088389.full
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author Turyahabwe Stavia
Barbara Castelnuovo
Christine Sekaggya-Wiltshire
Kelly E Dooley
Grace Banturaki
Irene Mbabazi
Ruth Mirembe Nabisere
Lucy Alinaitwe
Brian Otaalo
Florence Aber
Juliet Nampala
Rogers Owori
Josephine Bayigga
Jonathan Mayito
Eva Agnes Odongpiny Laker
Moorine Penninah Sekadde
Jotam Pasipanodya
Stella Zawedde‐Muyanja
author_facet Turyahabwe Stavia
Barbara Castelnuovo
Christine Sekaggya-Wiltshire
Kelly E Dooley
Grace Banturaki
Irene Mbabazi
Ruth Mirembe Nabisere
Lucy Alinaitwe
Brian Otaalo
Florence Aber
Juliet Nampala
Rogers Owori
Josephine Bayigga
Jonathan Mayito
Eva Agnes Odongpiny Laker
Moorine Penninah Sekadde
Jotam Pasipanodya
Stella Zawedde‐Muyanja
author_sort Turyahabwe Stavia
collection DOAJ
description Introduction Tuberculosis (TB) is the leading infectious cause of death globally. Despite WHO recommendations for TB preventive therapy (TPT), challenges persist, including incompletion of treatment and adverse drug reactions (ADRs). There is limited data on the 3-month isoniazid and rifapentine (3HP) pharmacokinetics, pharmacogenomics and their relation with ADRs. Our study aims to describe the pharmacokinetic and pharmacogenomics of 3HP used for TPT, the ADRs and their association with completion rates, and TPT outcomes, providing vital insights for TB control strategies in resource-limited settings.Methods This is an observational cohort study with a nested case–control study. We enrolled consecutive patients who had been initiated on TPT using the 3HP regimen. These are followed up biweekly and then monthly during the active phase of treatment and 3 monthly for 2 years following completion of TPT. ADR evaluation includes clinical assessment and liver function tests. Cases are selected from those who experience ADRs and controls from those who do not. Serum isoniazid and rifapentine concentrations are measured and pharmacogenomic analysis for NAT2, AADAC and CYP2E1 polymorphisms are done. Participants are followed up for 2 years to determine TPT outcomes.Analysis The safety profile of 3HP will be assessed using descriptive statistics, including proportions of patients experiencing ADRs and grade 3 or above events related to treatment. χ2 tests and regression models will determine predictors of ADRs and their impact on treatment completion. Pharmacokinetic-pharmacodynamic modelling will establish population parameters and factors influencing rifapentine and isoniazid concentrations.Ethics and dissemination Ethical approval of this study inclusive of all the appropriate documents was obtained from the Infectious Diseases Institute Research and Ethics Committee and the Uganda National Council of Science and Technology. The study adheres to legal, ethical and Good Clinical Practice (GCP) guidelines. Deidentified genotype data from 300 patients will be shared after publication. The protocol and phenotype data will be publicly accessible. Abstracts will be submitted to conferences, and a manuscript will be published poststudy.
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spelling doaj-art-fe70ad711514421eb6857b4cbbb5933f2025-08-20T02:40:02ZengBMJ Publishing GroupBMJ Open2044-60552024-12-01141210.1136/bmjopen-2024-088389Clinical predictors of 3-month isoniazid rifapentine (3HP)-related adverse drug reactions (ADR) during tuberculosis preventive therapy (PAnDoRA-3HP study): an observational study protocolTuryahabwe Stavia0Barbara Castelnuovo1Christine Sekaggya-Wiltshire2Kelly E Dooley3Grace Banturaki4Irene Mbabazi5Ruth Mirembe Nabisere6Lucy Alinaitwe7Brian Otaalo8Florence Aber9Juliet Nampala10Rogers Owori11Josephine Bayigga12Jonathan Mayito13Eva Agnes Odongpiny Laker14Moorine Penninah Sekadde15Jotam Pasipanodya16Stella Zawedde‐Muyanja17National Tuberculosis and Leprosy Program, Republic of Uganda Ministry of Health, Kampala, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaVanderbilt University Medical Center, Nashville, Tennessee, USAInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaNational TB and Leprosy Division, Republic of Uganda Ministry of Health, Kampala, UgandaVanderbilt University Medical Center, Nashville, Tennessee, USAInfectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Central, UgandaIntroduction Tuberculosis (TB) is the leading infectious cause of death globally. Despite WHO recommendations for TB preventive therapy (TPT), challenges persist, including incompletion of treatment and adverse drug reactions (ADRs). There is limited data on the 3-month isoniazid and rifapentine (3HP) pharmacokinetics, pharmacogenomics and their relation with ADRs. Our study aims to describe the pharmacokinetic and pharmacogenomics of 3HP used for TPT, the ADRs and their association with completion rates, and TPT outcomes, providing vital insights for TB control strategies in resource-limited settings.Methods This is an observational cohort study with a nested case–control study. We enrolled consecutive patients who had been initiated on TPT using the 3HP regimen. These are followed up biweekly and then monthly during the active phase of treatment and 3 monthly for 2 years following completion of TPT. ADR evaluation includes clinical assessment and liver function tests. Cases are selected from those who experience ADRs and controls from those who do not. Serum isoniazid and rifapentine concentrations are measured and pharmacogenomic analysis for NAT2, AADAC and CYP2E1 polymorphisms are done. Participants are followed up for 2 years to determine TPT outcomes.Analysis The safety profile of 3HP will be assessed using descriptive statistics, including proportions of patients experiencing ADRs and grade 3 or above events related to treatment. χ2 tests and regression models will determine predictors of ADRs and their impact on treatment completion. Pharmacokinetic-pharmacodynamic modelling will establish population parameters and factors influencing rifapentine and isoniazid concentrations.Ethics and dissemination Ethical approval of this study inclusive of all the appropriate documents was obtained from the Infectious Diseases Institute Research and Ethics Committee and the Uganda National Council of Science and Technology. The study adheres to legal, ethical and Good Clinical Practice (GCP) guidelines. Deidentified genotype data from 300 patients will be shared after publication. The protocol and phenotype data will be publicly accessible. Abstracts will be submitted to conferences, and a manuscript will be published poststudy.https://bmjopen.bmj.com/content/14/12/e088389.full
spellingShingle Turyahabwe Stavia
Barbara Castelnuovo
Christine Sekaggya-Wiltshire
Kelly E Dooley
Grace Banturaki
Irene Mbabazi
Ruth Mirembe Nabisere
Lucy Alinaitwe
Brian Otaalo
Florence Aber
Juliet Nampala
Rogers Owori
Josephine Bayigga
Jonathan Mayito
Eva Agnes Odongpiny Laker
Moorine Penninah Sekadde
Jotam Pasipanodya
Stella Zawedde‐Muyanja
Clinical predictors of 3-month isoniazid rifapentine (3HP)-related adverse drug reactions (ADR) during tuberculosis preventive therapy (PAnDoRA-3HP study): an observational study protocol
BMJ Open
title Clinical predictors of 3-month isoniazid rifapentine (3HP)-related adverse drug reactions (ADR) during tuberculosis preventive therapy (PAnDoRA-3HP study): an observational study protocol
title_full Clinical predictors of 3-month isoniazid rifapentine (3HP)-related adverse drug reactions (ADR) during tuberculosis preventive therapy (PAnDoRA-3HP study): an observational study protocol
title_fullStr Clinical predictors of 3-month isoniazid rifapentine (3HP)-related adverse drug reactions (ADR) during tuberculosis preventive therapy (PAnDoRA-3HP study): an observational study protocol
title_full_unstemmed Clinical predictors of 3-month isoniazid rifapentine (3HP)-related adverse drug reactions (ADR) during tuberculosis preventive therapy (PAnDoRA-3HP study): an observational study protocol
title_short Clinical predictors of 3-month isoniazid rifapentine (3HP)-related adverse drug reactions (ADR) during tuberculosis preventive therapy (PAnDoRA-3HP study): an observational study protocol
title_sort clinical predictors of 3 month isoniazid rifapentine 3hp related adverse drug reactions adr during tuberculosis preventive therapy pandora 3hp study an observational study protocol
url https://bmjopen.bmj.com/content/14/12/e088389.full
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