Correlation analysis between semen routine parameters and sperm DNA fragmentation index in patients with semen non-liquefaction: A retrospective study

This research investigated the correlation between routine semen parameters and the sperm DNA fragmentation index (DFI) in infertile males with non-liquefying semen, aiming to provide insights for infertility management. We conducted a retrospective analysis of 152 infertile males at our Andrology D...

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Main Authors: Ji Fengqing, Chen Junying, Lin Liyun
Format: Article
Language:English
Published: De Gruyter 2025-05-01
Series:Open Life Sciences
Subjects:
Online Access:https://doi.org/10.1515/biol-2022-1020
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author Ji Fengqing
Chen Junying
Lin Liyun
author_facet Ji Fengqing
Chen Junying
Lin Liyun
author_sort Ji Fengqing
collection DOAJ
description This research investigated the correlation between routine semen parameters and the sperm DNA fragmentation index (DFI) in infertile males with non-liquefying semen, aiming to provide insights for infertility management. We conducted a retrospective analysis of 152 infertile males at our Andrology Department from March 2023 to March 2024, assessing differences in semen parameters and DFI based on liquefaction times and identifying influential factors on DFI. Participants were divided into two groups based on their semen liquefaction times: less than 60 min (111 patients) and 60 min or more (41 patients). The group with normal liquefaction times demonstrated significantly better forward and total sperm motility, and lower sperm morphology indices and DFI values. A positive correlation was found between DFI and liquefaction time, while a negative correlation was observed with motility and normal sperm ratio. Specifically, each additional minute of liquefaction time increased DFI by 0.13, and a 1% decrease in normal sperm ratio increased DFI by 0.73. The findings indicate that delayed semen liquefaction correlates with poorer sperm motility and morphology and higher DFI, underscoring the importance of comprehensive semen assessment in evaluating male fertility.
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spelling doaj-art-fe6f89d3c0fd48e1b816adfdc679c9182025-08-20T02:34:13ZengDe GruyterOpen Life Sciences2391-54122025-05-01201374510.1515/biol-2022-1020Correlation analysis between semen routine parameters and sperm DNA fragmentation index in patients with semen non-liquefaction: A retrospective studyJi Fengqing0Chen Junying1Lin Liyun2Department of Clinical Laboratory, Xiamen Hospital of Traditional Chinese Medicine, 1739 Xianyue Road, Huli District, Xiamen, Fujian 361009, ChinaDepartment of Clinical Laboratory, Xiamen Hospital of Traditional Chinese Medicine, Xiamen, Fujian 361009, ChinaDepartment of Clinical Laboratory, Xiamen Hospital of Traditional Chinese Medicine, Xiamen, Fujian 361009, ChinaThis research investigated the correlation between routine semen parameters and the sperm DNA fragmentation index (DFI) in infertile males with non-liquefying semen, aiming to provide insights for infertility management. We conducted a retrospective analysis of 152 infertile males at our Andrology Department from March 2023 to March 2024, assessing differences in semen parameters and DFI based on liquefaction times and identifying influential factors on DFI. Participants were divided into two groups based on their semen liquefaction times: less than 60 min (111 patients) and 60 min or more (41 patients). The group with normal liquefaction times demonstrated significantly better forward and total sperm motility, and lower sperm morphology indices and DFI values. A positive correlation was found between DFI and liquefaction time, while a negative correlation was observed with motility and normal sperm ratio. Specifically, each additional minute of liquefaction time increased DFI by 0.13, and a 1% decrease in normal sperm ratio increased DFI by 0.73. The findings indicate that delayed semen liquefaction correlates with poorer sperm motility and morphology and higher DFI, underscoring the importance of comprehensive semen assessment in evaluating male fertility.https://doi.org/10.1515/biol-2022-1020infertilitysemen non-liquefactionsperm dna fragmentationroutine semen parameters
spellingShingle Ji Fengqing
Chen Junying
Lin Liyun
Correlation analysis between semen routine parameters and sperm DNA fragmentation index in patients with semen non-liquefaction: A retrospective study
Open Life Sciences
infertility
semen non-liquefaction
sperm dna fragmentation
routine semen parameters
title Correlation analysis between semen routine parameters and sperm DNA fragmentation index in patients with semen non-liquefaction: A retrospective study
title_full Correlation analysis between semen routine parameters and sperm DNA fragmentation index in patients with semen non-liquefaction: A retrospective study
title_fullStr Correlation analysis between semen routine parameters and sperm DNA fragmentation index in patients with semen non-liquefaction: A retrospective study
title_full_unstemmed Correlation analysis between semen routine parameters and sperm DNA fragmentation index in patients with semen non-liquefaction: A retrospective study
title_short Correlation analysis between semen routine parameters and sperm DNA fragmentation index in patients with semen non-liquefaction: A retrospective study
title_sort correlation analysis between semen routine parameters and sperm dna fragmentation index in patients with semen non liquefaction a retrospective study
topic infertility
semen non-liquefaction
sperm dna fragmentation
routine semen parameters
url https://doi.org/10.1515/biol-2022-1020
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AT chenjunying correlationanalysisbetweensemenroutineparametersandspermdnafragmentationindexinpatientswithsemennonliquefactionaretrospectivestudy
AT linliyun correlationanalysisbetweensemenroutineparametersandspermdnafragmentationindexinpatientswithsemennonliquefactionaretrospectivestudy