Comparative evaluation of echocardiographic and morphometric characteristics of the rat heart left ventricle
Purpose of the study. Comparative evaluation of echocardiographic and morphometric dimensions of the rat heart left ventricle. Materials and methods. The study included 10 outbred male rats weighing 160–180 g. The size of the heart left ventricle was assessed using echocardiography and morphometry....
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
LLC “Publisher OKI”
2023-05-01
|
| Series: | Фармакокинетика и Фармакодинамика |
| Subjects: | |
| Online Access: | https://www.pharmacokinetica.ru/jour/article/view/358 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849744356746985472 |
|---|---|
| author | E. O. Ionova I. A. Miroshkina A. V. Sorokina S. A. Kryzhanovskii |
| author_facet | E. O. Ionova I. A. Miroshkina A. V. Sorokina S. A. Kryzhanovskii |
| author_sort | E. O. Ionova |
| collection | DOAJ |
| description | Purpose of the study. Comparative evaluation of echocardiographic and morphometric dimensions of the rat heart left ventricle. Materials and methods. The study included 10 outbred male rats weighing 160–180 g. The size of the heart left ventricle was assessed using echocardiography and morphometry. The measurements were carried out according to standard protocols. To perform morphometric measurements, hearts were stopped in systole with a lethal dose (1.0 mg/kg) of 0.025 % strophanthin K solution, and in diastole, by immersing the hearts in a chilled physiological solution (calcium-free medium). Results. It was shown that the systolic size of the left ventricle of the heart according to echocardiography is 1.79Ѓ}0.10 mm, and morphometry 1.64Ѓ}0.09 mm (p = 0,302); diastolic size of the left ventricle, respectively, 3.42Ѓ}0.16 mm and 3.66Ѓ}0.17 mm (р = 0,318). The detected discrepancies do not exceed 10 % and, apparently, are due to the fact that the physiological dimensions of the heart left ventricle are measured by a non-invasive echocardiographic method, and for morphometric measurements of the dimensions in the systole, the heart was stopped by injection of strophanthin K, which entails an overload of heart cells with Ca2+ ions and as a result, contracture of cardiomyocytes; post-mortem cardiac arrest in diastole, in a calcium-free environment, is accompanied by a loss of cardiac muscle tone and, therefore, dilatation of the left ventricle will naturally be greater than physiological. Conclusion. Noninvasive echocardiographic measurement of the size of the heart left ventricle fully reflects the actual size of the left ventricle of the rat heart (the difference does not exceed 10 %), i. e. echocardiographic measurements are valid. |
| format | Article |
| id | doaj-art-fe6c583d8ecc4d4a8996fb04c412807d |
| institution | DOAJ |
| issn | 2587-7836 2686-8830 |
| language | Russian |
| publishDate | 2023-05-01 |
| publisher | LLC “Publisher OKI” |
| record_format | Article |
| series | Фармакокинетика и Фармакодинамика |
| spelling | doaj-art-fe6c583d8ecc4d4a8996fb04c412807d2025-08-20T03:19:57ZrusLLC “Publisher OKI”Фармакокинетика и Фармакодинамика2587-78362686-88302023-05-0101414410.37489/2587-7836-2023-1-41-44327Comparative evaluation of echocardiographic and morphometric characteristics of the rat heart left ventricleE. O. Ionova0I. A. Miroshkina1A. V. Sorokina2S. A. Kryzhanovskii3FSBI “Zakusov Institute of Pharmacology”FSBI “Zakusov Institute of Pharmacology”FSBI “Zakusov Institute of Pharmacology”FSBI “Zakusov Institute of Pharmacology”Purpose of the study. Comparative evaluation of echocardiographic and morphometric dimensions of the rat heart left ventricle. Materials and methods. The study included 10 outbred male rats weighing 160–180 g. The size of the heart left ventricle was assessed using echocardiography and morphometry. The measurements were carried out according to standard protocols. To perform morphometric measurements, hearts were stopped in systole with a lethal dose (1.0 mg/kg) of 0.025 % strophanthin K solution, and in diastole, by immersing the hearts in a chilled physiological solution (calcium-free medium). Results. It was shown that the systolic size of the left ventricle of the heart according to echocardiography is 1.79Ѓ}0.10 mm, and morphometry 1.64Ѓ}0.09 mm (p = 0,302); diastolic size of the left ventricle, respectively, 3.42Ѓ}0.16 mm and 3.66Ѓ}0.17 mm (р = 0,318). The detected discrepancies do not exceed 10 % and, apparently, are due to the fact that the physiological dimensions of the heart left ventricle are measured by a non-invasive echocardiographic method, and for morphometric measurements of the dimensions in the systole, the heart was stopped by injection of strophanthin K, which entails an overload of heart cells with Ca2+ ions and as a result, contracture of cardiomyocytes; post-mortem cardiac arrest in diastole, in a calcium-free environment, is accompanied by a loss of cardiac muscle tone and, therefore, dilatation of the left ventricle will naturally be greater than physiological. Conclusion. Noninvasive echocardiographic measurement of the size of the heart left ventricle fully reflects the actual size of the left ventricle of the rat heart (the difference does not exceed 10 %), i. e. echocardiographic measurements are valid.https://www.pharmacokinetica.ru/jour/article/view/358echocardiographymorphometrysystolic size of the left ventricle of the heartdiastolic size of the left ventricle of the heartvalidity |
| spellingShingle | E. O. Ionova I. A. Miroshkina A. V. Sorokina S. A. Kryzhanovskii Comparative evaluation of echocardiographic and morphometric characteristics of the rat heart left ventricle Фармакокинетика и Фармакодинамика echocardiography morphometry systolic size of the left ventricle of the heart diastolic size of the left ventricle of the heart validity |
| title | Comparative evaluation of echocardiographic and morphometric characteristics of the rat heart left ventricle |
| title_full | Comparative evaluation of echocardiographic and morphometric characteristics of the rat heart left ventricle |
| title_fullStr | Comparative evaluation of echocardiographic and morphometric characteristics of the rat heart left ventricle |
| title_full_unstemmed | Comparative evaluation of echocardiographic and morphometric characteristics of the rat heart left ventricle |
| title_short | Comparative evaluation of echocardiographic and morphometric characteristics of the rat heart left ventricle |
| title_sort | comparative evaluation of echocardiographic and morphometric characteristics of the rat heart left ventricle |
| topic | echocardiography morphometry systolic size of the left ventricle of the heart diastolic size of the left ventricle of the heart validity |
| url | https://www.pharmacokinetica.ru/jour/article/view/358 |
| work_keys_str_mv | AT eoionova comparativeevaluationofechocardiographicandmorphometriccharacteristicsoftheratheartleftventricle AT iamiroshkina comparativeevaluationofechocardiographicandmorphometriccharacteristicsoftheratheartleftventricle AT avsorokina comparativeevaluationofechocardiographicandmorphometriccharacteristicsoftheratheartleftventricle AT sakryzhanovskii comparativeevaluationofechocardiographicandmorphometriccharacteristicsoftheratheartleftventricle |