DNA in a bottle-Rapid metabarcoding survey for early alerts of invasive species in ports.
Biota monitoring in ports is increasingly needed for biosecurity reasons and safeguarding marine biodiversity from biological invasion. Present and future international biosecurity directives can be accomplished only if the biota acquired by maritime traffic in ports is controlled. Methodologies for...
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183347&type=printable |
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| author | Yaisel J Borrell Laura Miralles Hoang Do Huu Khaled Mohammed-Geba Eva Garcia-Vazquez |
| author_facet | Yaisel J Borrell Laura Miralles Hoang Do Huu Khaled Mohammed-Geba Eva Garcia-Vazquez |
| author_sort | Yaisel J Borrell |
| collection | DOAJ |
| description | Biota monitoring in ports is increasingly needed for biosecurity reasons and safeguarding marine biodiversity from biological invasion. Present and future international biosecurity directives can be accomplished only if the biota acquired by maritime traffic in ports is controlled. Methodologies for biota inventory are diverse and now rely principally on extensive and labor-intensive sampling along with taxonomic identification by experts. In this study, we employed an extremely simplified environmental DNA (eDNA) sampling methodology from only three 1-L bottles of water per port, followed by metabarcoding (high-throughput sequencing and DNA-based species identification) using 18S rDNA and Cytochrome oxidase I as genetic barcodes. Eight Bay of Biscay ports with available inventory of fouling invertebrates were employed as a case study. Despite minimal sampling efforts, three invasive invertebrates were detected: the barnacle Austrominius modestus, the tubeworm Ficopomatus enigmaticus and the polychaete Polydora triglanda. The same species have been previously found from visual and DNA barcoding (genetic identification of individuals) surveys in the same ports. The current costs of visual surveys, conventional DNA barcoding and this simplified metabarcoding protocol were compared. The results encourage the use of metabarcoding for early biosecurity alerts. |
| format | Article |
| id | doaj-art-fe6bf574baee40fba77f2d117651c55d |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-fe6bf574baee40fba77f2d117651c55d2025-08-20T02:45:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018334710.1371/journal.pone.0183347DNA in a bottle-Rapid metabarcoding survey for early alerts of invasive species in ports.Yaisel J BorrellLaura MirallesHoang Do HuuKhaled Mohammed-GebaEva Garcia-VazquezBiota monitoring in ports is increasingly needed for biosecurity reasons and safeguarding marine biodiversity from biological invasion. Present and future international biosecurity directives can be accomplished only if the biota acquired by maritime traffic in ports is controlled. Methodologies for biota inventory are diverse and now rely principally on extensive and labor-intensive sampling along with taxonomic identification by experts. In this study, we employed an extremely simplified environmental DNA (eDNA) sampling methodology from only three 1-L bottles of water per port, followed by metabarcoding (high-throughput sequencing and DNA-based species identification) using 18S rDNA and Cytochrome oxidase I as genetic barcodes. Eight Bay of Biscay ports with available inventory of fouling invertebrates were employed as a case study. Despite minimal sampling efforts, three invasive invertebrates were detected: the barnacle Austrominius modestus, the tubeworm Ficopomatus enigmaticus and the polychaete Polydora triglanda. The same species have been previously found from visual and DNA barcoding (genetic identification of individuals) surveys in the same ports. The current costs of visual surveys, conventional DNA barcoding and this simplified metabarcoding protocol were compared. The results encourage the use of metabarcoding for early biosecurity alerts.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183347&type=printable |
| spellingShingle | Yaisel J Borrell Laura Miralles Hoang Do Huu Khaled Mohammed-Geba Eva Garcia-Vazquez DNA in a bottle-Rapid metabarcoding survey for early alerts of invasive species in ports. PLoS ONE |
| title | DNA in a bottle-Rapid metabarcoding survey for early alerts of invasive species in ports. |
| title_full | DNA in a bottle-Rapid metabarcoding survey for early alerts of invasive species in ports. |
| title_fullStr | DNA in a bottle-Rapid metabarcoding survey for early alerts of invasive species in ports. |
| title_full_unstemmed | DNA in a bottle-Rapid metabarcoding survey for early alerts of invasive species in ports. |
| title_short | DNA in a bottle-Rapid metabarcoding survey for early alerts of invasive species in ports. |
| title_sort | dna in a bottle rapid metabarcoding survey for early alerts of invasive species in ports |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183347&type=printable |
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