Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B
Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety. Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L. monocytogenes by interacting with the cell membrane. This research further explored the antibacterial...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002567 |
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| author | Yangliu Liu Yawei Ning Zhou Chen Panpan Han Tongxin Zhi Siting Li Aijin Ma Yingmin Jia |
| author_facet | Yangliu Liu Yawei Ning Zhou Chen Panpan Han Tongxin Zhi Siting Li Aijin Ma Yingmin Jia |
| author_sort | Yangliu Liu |
| collection | DOAJ |
| description | Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety. Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L. monocytogenes by interacting with the cell membrane. This research further explored the antibacterial mechanism of brevilaterin B against L. monocytogenes at the sub-minimum inhibition concentration via transcriptomic analysis. Brevilaterin B induced growth inhibition rather than direct membrane lysis in L. monocytogenes at the minimum inhibitory concentration. Transcriptomic analysis showed 1779 difference expressed genes, including 895 up-regulated and 884 down-regulated genes. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that brevilaterin B influenced multiple pathways of L. monocytogenes, including peptidoglycan biosynthesis, membrane transport (ATP-binding cassette transports, ion transport), cellular metabolism (amino acid and lipid metabolism), ATP synthesis, and activation of the stress response (quorum sensing and bacterial chemotaxis). In conclusion, brevilaterin B affects gene expression related to biosynthesis, transport and stress response pathways on the membrane of L. monocytogenes. The present work provides the first transcriptomic assessment of the antibacterial mechanism of lipopeptide brevilaterin B at the gene level. |
| format | Article |
| id | doaj-art-fe69fda42ac34498897c393122386988 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-fe69fda42ac34498897c3931223869882025-02-03T11:09:30ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112413591368Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin BYangliu Liu0Yawei Ning1Zhou Chen2Panpan Han3Tongxin Zhi4Siting Li5Aijin Ma6Yingmin Jia7School of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaCollege of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, China; Corresponding authors at: School of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, China; Corresponding authors at: School of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaListeria monocytogenes is a worrisome food-borne pathogen threatening global food safety. Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L. monocytogenes by interacting with the cell membrane. This research further explored the antibacterial mechanism of brevilaterin B against L. monocytogenes at the sub-minimum inhibition concentration via transcriptomic analysis. Brevilaterin B induced growth inhibition rather than direct membrane lysis in L. monocytogenes at the minimum inhibitory concentration. Transcriptomic analysis showed 1779 difference expressed genes, including 895 up-regulated and 884 down-regulated genes. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that brevilaterin B influenced multiple pathways of L. monocytogenes, including peptidoglycan biosynthesis, membrane transport (ATP-binding cassette transports, ion transport), cellular metabolism (amino acid and lipid metabolism), ATP synthesis, and activation of the stress response (quorum sensing and bacterial chemotaxis). In conclusion, brevilaterin B affects gene expression related to biosynthesis, transport and stress response pathways on the membrane of L. monocytogenes. The present work provides the first transcriptomic assessment of the antibacterial mechanism of lipopeptide brevilaterin B at the gene level.http://www.sciencedirect.com/science/article/pii/S2213453022002567Antimicrobial lipopeptideBrevilaterin BAntibacterial mechanismListeria monocytogenesTranscriptomics |
| spellingShingle | Yangliu Liu Yawei Ning Zhou Chen Panpan Han Tongxin Zhi Siting Li Aijin Ma Yingmin Jia Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B Food Science and Human Wellness Antimicrobial lipopeptide Brevilaterin B Antibacterial mechanism Listeria monocytogenes Transcriptomics |
| title | Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B |
| title_full | Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B |
| title_fullStr | Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B |
| title_full_unstemmed | Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B |
| title_short | Transcriptomics reveals substance biosynthesis and transport on membranes of Listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin B |
| title_sort | transcriptomics reveals substance biosynthesis and transport on membranes of listeria monocytogenes affected by antimicrobial lipopeptide brevilaterin b |
| topic | Antimicrobial lipopeptide Brevilaterin B Antibacterial mechanism Listeria monocytogenes Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002567 |
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