Maternal supplementation with selenium nanoparticles enhances reproductive outcomes and larval quality in Asian seabass (Lates calcarifer) by influencing gene expression and physiological parameters
Selenium plays vital roles in antioxidant defense, immune function, and reproductive physiology in vertebrates. This study evaluated the impacts of dietary selenium nanoparticles (SeNPs) on reproductive outcomes, steroidogenesis, vitellogenesis, and larval quality in female Asian seabass (Lates calc...
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003199 |
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| author | Seyed Ali Khorasaninasab Saeed Keyvanshokooh Mansour Torfi Mozanzadeh Ahmad Ghasemi Mahdi Naderi Samira Nazemroaya |
| author_facet | Seyed Ali Khorasaninasab Saeed Keyvanshokooh Mansour Torfi Mozanzadeh Ahmad Ghasemi Mahdi Naderi Samira Nazemroaya |
| author_sort | Seyed Ali Khorasaninasab |
| collection | DOAJ |
| description | Selenium plays vital roles in antioxidant defense, immune function, and reproductive physiology in vertebrates. This study evaluated the impacts of dietary selenium nanoparticles (SeNPs) on reproductive outcomes, steroidogenesis, vitellogenesis, and larval quality in female Asian seabass (Lates calcarifer), by influencing gene expression, antioxidant capacity, hormone levels, and reproductive indices. Female broodfish (n = 12; 1990 ± 120 g) were fed either a basal diet (control) or a basal diet supplemented with 2 mg SeNPs/kg (SeNPs-2) for 120 days before spawning. Supplementation with SeNPs significantly (P < 0.05) increased Se deposition in the liver, ovary, and larvae, enhancing antioxidant capacity (glutathione peroxidase and reduced-glutathione), and reducing oxidative stress marker (malondialdehyde; MDA) in both broodfish and offspring. The expression levels of genes involved in steroidogenesis (star, P450scc, and 3β-hsd) and vitellogenesis (zp2 and vtg) were upregulated in the ovary or liver of the SeNPs-2 group (P < 0.05). Despite comparable serum testosterone and estradiol levels (P > 0.05), broodfish fed the SeNPs-2 diet exhibited significantly (P < 0.05) lower progesterone levels, and lower serum cholesterol and triglyceride levels. Reproductive performance, as indicated by spawning frequency, relative fecundity, fertilization rate, and hatching rate, was significantly (P < 0.05) improved in the SeNPs-2 group. Larvae descending from the SeNPs-fed broodfish exhibited higher expression of growth-promoting genes (GH and IGF-I), larger body size, and better developmental traits than those from the control group. These findings suggest that dietary SeNP supplementation positively influences Se bioavailability, gene expression, reproductive efficiency, and larval quality in L. calcarifer. SeNPs represent a promising nutritional strategy for optimizing broodstock performance and enhancing offspring quality in aquaculture. |
| format | Article |
| id | doaj-art-fe67108767aa403bae5f331124affc83 |
| institution | DOAJ |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-fe67108767aa403bae5f331124affc832025-08-20T02:57:08ZengElsevierAquaculture Reports2352-51342025-09-014310293310.1016/j.aqrep.2025.102933Maternal supplementation with selenium nanoparticles enhances reproductive outcomes and larval quality in Asian seabass (Lates calcarifer) by influencing gene expression and physiological parametersSeyed Ali Khorasaninasab0Saeed Keyvanshokooh1Mansour Torfi Mozanzadeh2Ahmad Ghasemi3Mahdi Naderi4Samira Nazemroaya5Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, IranDepartment of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran; Corresponding authors.South of Iran Aquaculture Research Centre, Iranian Fisheries Science Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Ahwaz, Iran; Corresponding authors.Department of Biotechnology, Persian Gulf Research Institute, Persian Gulf University, Bushehr, IranDepartment of Fisheries, Iran Fisheries Organization, Behbahan, Khuzestan, IranSouth of Iran Aquaculture Research Centre, Iranian Fisheries Science Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Ahwaz, IranSelenium plays vital roles in antioxidant defense, immune function, and reproductive physiology in vertebrates. This study evaluated the impacts of dietary selenium nanoparticles (SeNPs) on reproductive outcomes, steroidogenesis, vitellogenesis, and larval quality in female Asian seabass (Lates calcarifer), by influencing gene expression, antioxidant capacity, hormone levels, and reproductive indices. Female broodfish (n = 12; 1990 ± 120 g) were fed either a basal diet (control) or a basal diet supplemented with 2 mg SeNPs/kg (SeNPs-2) for 120 days before spawning. Supplementation with SeNPs significantly (P < 0.05) increased Se deposition in the liver, ovary, and larvae, enhancing antioxidant capacity (glutathione peroxidase and reduced-glutathione), and reducing oxidative stress marker (malondialdehyde; MDA) in both broodfish and offspring. The expression levels of genes involved in steroidogenesis (star, P450scc, and 3β-hsd) and vitellogenesis (zp2 and vtg) were upregulated in the ovary or liver of the SeNPs-2 group (P < 0.05). Despite comparable serum testosterone and estradiol levels (P > 0.05), broodfish fed the SeNPs-2 diet exhibited significantly (P < 0.05) lower progesterone levels, and lower serum cholesterol and triglyceride levels. Reproductive performance, as indicated by spawning frequency, relative fecundity, fertilization rate, and hatching rate, was significantly (P < 0.05) improved in the SeNPs-2 group. Larvae descending from the SeNPs-fed broodfish exhibited higher expression of growth-promoting genes (GH and IGF-I), larger body size, and better developmental traits than those from the control group. These findings suggest that dietary SeNP supplementation positively influences Se bioavailability, gene expression, reproductive efficiency, and larval quality in L. calcarifer. SeNPs represent a promising nutritional strategy for optimizing broodstock performance and enhancing offspring quality in aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513425003199Antioxidant capacityGrowth-related genesMaternal transferSteroid hormonesVitellogenesis |
| spellingShingle | Seyed Ali Khorasaninasab Saeed Keyvanshokooh Mansour Torfi Mozanzadeh Ahmad Ghasemi Mahdi Naderi Samira Nazemroaya Maternal supplementation with selenium nanoparticles enhances reproductive outcomes and larval quality in Asian seabass (Lates calcarifer) by influencing gene expression and physiological parameters Aquaculture Reports Antioxidant capacity Growth-related genes Maternal transfer Steroid hormones Vitellogenesis |
| title | Maternal supplementation with selenium nanoparticles enhances reproductive outcomes and larval quality in Asian seabass (Lates calcarifer) by influencing gene expression and physiological parameters |
| title_full | Maternal supplementation with selenium nanoparticles enhances reproductive outcomes and larval quality in Asian seabass (Lates calcarifer) by influencing gene expression and physiological parameters |
| title_fullStr | Maternal supplementation with selenium nanoparticles enhances reproductive outcomes and larval quality in Asian seabass (Lates calcarifer) by influencing gene expression and physiological parameters |
| title_full_unstemmed | Maternal supplementation with selenium nanoparticles enhances reproductive outcomes and larval quality in Asian seabass (Lates calcarifer) by influencing gene expression and physiological parameters |
| title_short | Maternal supplementation with selenium nanoparticles enhances reproductive outcomes and larval quality in Asian seabass (Lates calcarifer) by influencing gene expression and physiological parameters |
| title_sort | maternal supplementation with selenium nanoparticles enhances reproductive outcomes and larval quality in asian seabass lates calcarifer by influencing gene expression and physiological parameters |
| topic | Antioxidant capacity Growth-related genes Maternal transfer Steroid hormones Vitellogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425003199 |
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