The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality
Background and Aim: The success of semen cryopreservation relies on several aspects, including breed, age, season, collection method, extender composition, cooling rate, equilibration period, freezing rate, and thawing rate. This study aimed to investigate the effects of cooling and equilibration du...
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Veterinary World
2024-12-01
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| Online Access: | https://www.veterinaryworld.org/Vol.17/December-2024/25.pdf |
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| author | Wilasinee Inyawilert Yu-Jing Liao Oswald Nfor Ndi Koranit Pradithera Atchawut Saengtun Sureeporn Saengwong Payungsuk Intawicha Kunlayaphat Wuthijaree Vorawatt Hanthongkul Kaikaew Kamdee Anurak Khieokhajonkhet Chalothon Amporn Attapol Tiantong Chompunut Lumsangkul |
| author_facet | Wilasinee Inyawilert Yu-Jing Liao Oswald Nfor Ndi Koranit Pradithera Atchawut Saengtun Sureeporn Saengwong Payungsuk Intawicha Kunlayaphat Wuthijaree Vorawatt Hanthongkul Kaikaew Kamdee Anurak Khieokhajonkhet Chalothon Amporn Attapol Tiantong Chompunut Lumsangkul |
| author_sort | Wilasinee Inyawilert |
| collection | DOAJ |
| description | Background and Aim: The success of semen cryopreservation relies on several aspects, including breed, age, season, collection method, extender composition, cooling rate, equilibration period, freezing rate, and thawing rate. This study aimed to investigate the effects of cooling and equilibration duration, as well as the addition of antioxidants to the semen extender, on the cryopreservation of swamp buffalo semen.
Materials and Methods: Semen collected from swamp buffalo bulls was subjected to four different conditions: (T1) 2-h cooling and 2-h equilibration, (T2) 1.5-h cooling and 1.5-h equilibration, (T3) 1-h cooling and 1-h equilibration, and (T4) 0.5-h cooling and 0.5-h equilibration. Spermatozoa motility was evaluated using a computer-assisted semen analyzer. Moreover, this study also investigated the effect of antioxidant supplementation during cryopreservation using tris-citrate egg yolk extenders enriched with various antioxidants: Control (Con), 1 mM melatonin (ML), 0.5 mM gamma-oryzanol (GO), 10 μM canthaxanthin (CX), 1 mM melatonin + 0.5 mM gamma-oryzanol (ML + GO), and 1 mM melatonin + 10 μM canthaxanthin (ML + CX).
Results: Results showed that the (T1) 2-h cooling and 2-h equilibration and (T2) 1.5-h cooling and 1.5-h equilibration groups achieved higher progressive motility than the (T3) 1-h cooling and 1-h equilibration and (T4) 0.5-h cooling and 0.5-h equilibration groups. The ML-treated group exhibited superior progressive motility and total motility.
Conclusion: The optimal approach for cryopreserving swamp buffalo bull semen involves a 1.5-h cooling period followed by a 1.5-h equilibration period, with the incorporation of ML into the semen extender. |
| format | Article |
| id | doaj-art-fe5f7306094e4af9b1fa9bc24203d955 |
| institution | DOAJ |
| issn | 0972-8988 2231-0916 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj-art-fe5f7306094e4af9b1fa9bc24203d9552025-08-20T02:52:49ZengVeterinary WorldVeterinary World0972-89882231-09162024-12-0117122950295610.14202/vetworld.2024.2950-2956The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation qualityWilasinee Inyawilert0https://orcid.org/0000-0002-0176-8009Yu-Jing Liao1https://orcid.org/0000-0003-0421-3268Oswald Nfor Ndi2https://orcid.org/0000-0002-4480-7098Koranit Pradithera3https://orcid.org/0009-0007-2163-8109Atchawut Saengtun4https://orcid.org/0009-0005-4319-1825Sureeporn Saengwong5https://orcid.org/0000-0003-0914-0942Payungsuk Intawicha6https://orcid.org/0000-0002-0314-0813Kunlayaphat Wuthijaree7https://orcid.org/0000-0001-5184-6258Vorawatt Hanthongkul8https://orcid.org/0009-0006-8413-4904Kaikaew Kamdee9https://orcid.org/0009-0002-1999-8168Anurak Khieokhajonkhet10https://orcid.org/0000-0003-4149-8821Chalothon Amporn11https://orcid.org/0009-0005-9434-246XAttapol Tiantong12https://orcid.org/0000-0001-9338-5162Chompunut Lumsangkul13https://orcid.org/0000-0003-2014-5306Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand; The Center for Agricultural Biotechnology, Naresuan University, Phitsanulok, Thailand.Genetics and Physiology Division, Taiwan Livestock Research Institute, Ministry of Agriculture, Tainan, Taiwan.Department of Public Health, Institute of Public Health, Chung Shan Medical University, Taichung, Taiwan.Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand.Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand.Program of Animal Science, School of Agriculture and Natural Resources, University of Phayao, Phayao, Thailand.Program of Animal Science, School of Agriculture and Natural Resources, University of Phayao, Phayao, Thailand.Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand.Phitsanulok Artificial Insemination and Biotechnology Research Center, Phitsanulok, Thailand.Phitsanulok Artificial Insemination and Biotechnology Research Center, Phitsanulok, Thailand.Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand; The Center for Agricultural Biotechnology, Naresuan University, Phitsanulok, Thailand.Department of Veterinary Technology, Kalasin University, Kalasin, Thailand.Faculty of Animal Sciences and Agricultural Technology, Silpakorn University, Phetchaburi IT Campus, Cha-Am, Phetchaburi, Thailand.Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.Background and Aim: The success of semen cryopreservation relies on several aspects, including breed, age, season, collection method, extender composition, cooling rate, equilibration period, freezing rate, and thawing rate. This study aimed to investigate the effects of cooling and equilibration duration, as well as the addition of antioxidants to the semen extender, on the cryopreservation of swamp buffalo semen. Materials and Methods: Semen collected from swamp buffalo bulls was subjected to four different conditions: (T1) 2-h cooling and 2-h equilibration, (T2) 1.5-h cooling and 1.5-h equilibration, (T3) 1-h cooling and 1-h equilibration, and (T4) 0.5-h cooling and 0.5-h equilibration. Spermatozoa motility was evaluated using a computer-assisted semen analyzer. Moreover, this study also investigated the effect of antioxidant supplementation during cryopreservation using tris-citrate egg yolk extenders enriched with various antioxidants: Control (Con), 1 mM melatonin (ML), 0.5 mM gamma-oryzanol (GO), 10 μM canthaxanthin (CX), 1 mM melatonin + 0.5 mM gamma-oryzanol (ML + GO), and 1 mM melatonin + 10 μM canthaxanthin (ML + CX). Results: Results showed that the (T1) 2-h cooling and 2-h equilibration and (T2) 1.5-h cooling and 1.5-h equilibration groups achieved higher progressive motility than the (T3) 1-h cooling and 1-h equilibration and (T4) 0.5-h cooling and 0.5-h equilibration groups. The ML-treated group exhibited superior progressive motility and total motility. Conclusion: The optimal approach for cryopreserving swamp buffalo bull semen involves a 1.5-h cooling period followed by a 1.5-h equilibration period, with the incorporation of ML into the semen extender.https://www.veterinaryworld.org/Vol.17/December-2024/25.pdfantioxidantcryopreservationequilibrationsemenand swamp buffalo |
| spellingShingle | Wilasinee Inyawilert Yu-Jing Liao Oswald Nfor Ndi Koranit Pradithera Atchawut Saengtun Sureeporn Saengwong Payungsuk Intawicha Kunlayaphat Wuthijaree Vorawatt Hanthongkul Kaikaew Kamdee Anurak Khieokhajonkhet Chalothon Amporn Attapol Tiantong Chompunut Lumsangkul The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality Veterinary World antioxidant cryopreservation equilibration semen and swamp buffalo |
| title | The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality |
| title_full | The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality |
| title_fullStr | The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality |
| title_full_unstemmed | The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality |
| title_short | The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality |
| title_sort | optimal combination of cooling and equilibration durations along with the addition of melatonin gamma oryzanol and canthaxanthin for improving swamp buffalo semen cryopreservation quality |
| topic | antioxidant cryopreservation equilibration semen and swamp buffalo |
| url | https://www.veterinaryworld.org/Vol.17/December-2024/25.pdf |
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