In vivo anti-aging properties on fat diet-induced high fat Drosophila melanogaster of n-butanol extract from Paecilomyces hepiali
The purpose of this study was to explore the potential of the development and application of Paecilomyces hepiali, a fungus with edible and medicinal value, as a foodborne antioxidant and anti-aging agent. Its n-butanol extract (PHE) from rice cultures was selected for anti-aging experiment because...
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002348 |
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| author | Akang Dan Yushi Chen Yongqi Tian Shaoyun Wang |
| author_facet | Akang Dan Yushi Chen Yongqi Tian Shaoyun Wang |
| author_sort | Akang Dan |
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| description | The purpose of this study was to explore the potential of the development and application of Paecilomyces hepiali, a fungus with edible and medicinal value, as a foodborne antioxidant and anti-aging agent. Its n-butanol extract (PHE) from rice cultures was selected for anti-aging experiment because of significant free radical scavenging activity in vitro. In vivo, PHE could significantly prolong the mean lifespan, 50 % survival days, and the maximum lifespan of Drosophila on a high-fat diet. It is amazing that the mean lifespan increased from 19.1 days to 32.9 days, 50 % survival days increased from 15.7 days to 34.3 days, and the maximum lifespan extended from 44.7 days to 52.7 days, when the high-fat female Drosophila model was fed with 10 μg/mL PHE. Further research showed that PHE reduced the accumulation of peroxide products and increased the activity of antioxidant enzymes. Then, through antioxidant activity tracking, dimerumic acid (compound 1, the IC50 value of 3.4 μg/mL on DPPH free radicals scavenging activity), 4,5-dihydroxy-3-methoxypentanoic acid (compound 2, new compound), and thymidine (compound 3) were isolated from PHE. It is worth mentioning that dimerumic acid, the major antioxidant compound of PHE (content up to 3 %), was discovered in P. hepiali for the first time. It was concluded that PHE showed excellent anti-aging activity at a very low concentration on fat diet-induced high fat Drosophila melanogaster, and dimerumic acid may be its main material basis. These results indicated that PHE had the potential to be developed as antioxidant and anti-aging agent in the healthcare industry. |
| format | Article |
| id | doaj-art-fe5669591aee472a9836d478ace6337f |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-fe5669591aee472a9836d478ace6337f2025-02-03T11:09:29ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112412041211In vivo anti-aging properties on fat diet-induced high fat Drosophila melanogaster of n-butanol extract from Paecilomyces hepialiAkang Dan0Yushi Chen1Yongqi Tian2Shaoyun Wang3College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China; College of Chemical, Fuzhou University, Fuzhou 350108, ChinaCollege of Biological Science and Technology, Fuzhou University, Fuzhou 350108, ChinaCollege of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China; Corresponding authors at: College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, ChinaCollege of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China; Corresponding authors at: College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, ChinaThe purpose of this study was to explore the potential of the development and application of Paecilomyces hepiali, a fungus with edible and medicinal value, as a foodborne antioxidant and anti-aging agent. Its n-butanol extract (PHE) from rice cultures was selected for anti-aging experiment because of significant free radical scavenging activity in vitro. In vivo, PHE could significantly prolong the mean lifespan, 50 % survival days, and the maximum lifespan of Drosophila on a high-fat diet. It is amazing that the mean lifespan increased from 19.1 days to 32.9 days, 50 % survival days increased from 15.7 days to 34.3 days, and the maximum lifespan extended from 44.7 days to 52.7 days, when the high-fat female Drosophila model was fed with 10 μg/mL PHE. Further research showed that PHE reduced the accumulation of peroxide products and increased the activity of antioxidant enzymes. Then, through antioxidant activity tracking, dimerumic acid (compound 1, the IC50 value of 3.4 μg/mL on DPPH free radicals scavenging activity), 4,5-dihydroxy-3-methoxypentanoic acid (compound 2, new compound), and thymidine (compound 3) were isolated from PHE. It is worth mentioning that dimerumic acid, the major antioxidant compound of PHE (content up to 3 %), was discovered in P. hepiali for the first time. It was concluded that PHE showed excellent anti-aging activity at a very low concentration on fat diet-induced high fat Drosophila melanogaster, and dimerumic acid may be its main material basis. These results indicated that PHE had the potential to be developed as antioxidant and anti-aging agent in the healthcare industry.http://www.sciencedirect.com/science/article/pii/S2213453022002348Paecilomyces hepialiAnti-agingDrosophilaDimerumic acid |
| spellingShingle | Akang Dan Yushi Chen Yongqi Tian Shaoyun Wang In vivo anti-aging properties on fat diet-induced high fat Drosophila melanogaster of n-butanol extract from Paecilomyces hepiali Food Science and Human Wellness Paecilomyces hepiali Anti-aging Drosophila Dimerumic acid |
| title | In vivo anti-aging properties on fat diet-induced high fat Drosophila melanogaster of n-butanol extract from Paecilomyces hepiali |
| title_full | In vivo anti-aging properties on fat diet-induced high fat Drosophila melanogaster of n-butanol extract from Paecilomyces hepiali |
| title_fullStr | In vivo anti-aging properties on fat diet-induced high fat Drosophila melanogaster of n-butanol extract from Paecilomyces hepiali |
| title_full_unstemmed | In vivo anti-aging properties on fat diet-induced high fat Drosophila melanogaster of n-butanol extract from Paecilomyces hepiali |
| title_short | In vivo anti-aging properties on fat diet-induced high fat Drosophila melanogaster of n-butanol extract from Paecilomyces hepiali |
| title_sort | in vivo anti aging properties on fat diet induced high fat drosophila melanogaster of n butanol extract from paecilomyces hepiali |
| topic | Paecilomyces hepiali Anti-aging Drosophila Dimerumic acid |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002348 |
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