Pharmacokinetics of Photogem Using Fluorescence Spectroscopy in Dimethylhydrazine-Induced Murine Colorectal Carcinoma
This study aimed to investigate the pharmacokinetics of a hematoporphyrin derivative in colonic tumors induced by dimethylhydrazine and adjacent normal colon in Wistar rats using an in vivo fluorescence spectroscopy technique. In conventional clinical application of photodynamic therapy, the interva...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/2012/615259 |
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| _version_ | 1850162506152017920 |
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| author | Raduan Hage Juliana Ferreira Vanderlei Salvador Bagnato José Dirceu Vollet-Filho Hélio Plapler |
| author_facet | Raduan Hage Juliana Ferreira Vanderlei Salvador Bagnato José Dirceu Vollet-Filho Hélio Plapler |
| author_sort | Raduan Hage |
| collection | DOAJ |
| description | This study aimed to investigate the pharmacokinetics of a hematoporphyrin derivative in colonic tumors induced by dimethylhydrazine and adjacent normal colon in Wistar rats using an in vivo fluorescence spectroscopy technique. In conventional clinical application of photodynamic therapy, the interval between photosensitizer (PS) administration and lesion illumination is often standardized without taking into account variations due to the type or localization of the tumor and intrinsic differences in the microcirculation and vascular permeability of each target organ. The analysis of the fluorescence spectra was based on the intensity of porphyrin emission band centered at around 620 nm in normal colon and colon tumors. The photosensitizer fluorescence intensity rapidly grew for carcinoma and normal colon, reaching the maximum values 1 and 3 hours after PS injection, respectively. Data presented here allow us to verify that the best compromise between selectivity and drug concentration for colon carcinoma in rats took place in the interval between 1 to 4 h after PS injection. |
| format | Article |
| id | doaj-art-fe5457d8ea86425a9f0a33ea381d1377 |
| institution | OA Journals |
| issn | 1110-662X 1687-529X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Photoenergy |
| spelling | doaj-art-fe5457d8ea86425a9f0a33ea381d13772025-08-20T02:22:33ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2012-01-01201210.1155/2012/615259615259Pharmacokinetics of Photogem Using Fluorescence Spectroscopy in Dimethylhydrazine-Induced Murine Colorectal CarcinomaRaduan Hage0Juliana Ferreira1Vanderlei Salvador Bagnato2José Dirceu Vollet-Filho3Hélio Plapler4Departamento de Cirurgia, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu 740, 04023-900 São Paulo, SP, BrazilInstituto de Pesquisa e Desenvolvimento—IP&D, Universidade do Vale do Paraíba (UNIVAP), Avenida Shishima Hifumi 2911, 12244-000 São José dos Campos, SP, BrazilInstituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador São Carlense 400, 13566-590 São Carlos, SP, BrazilInstituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador São Carlense 400, 13566-590 São Carlos, SP, BrazilDepartamento de Cirurgia, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu 740, 04023-900 São Paulo, SP, BrazilThis study aimed to investigate the pharmacokinetics of a hematoporphyrin derivative in colonic tumors induced by dimethylhydrazine and adjacent normal colon in Wistar rats using an in vivo fluorescence spectroscopy technique. In conventional clinical application of photodynamic therapy, the interval between photosensitizer (PS) administration and lesion illumination is often standardized without taking into account variations due to the type or localization of the tumor and intrinsic differences in the microcirculation and vascular permeability of each target organ. The analysis of the fluorescence spectra was based on the intensity of porphyrin emission band centered at around 620 nm in normal colon and colon tumors. The photosensitizer fluorescence intensity rapidly grew for carcinoma and normal colon, reaching the maximum values 1 and 3 hours after PS injection, respectively. Data presented here allow us to verify that the best compromise between selectivity and drug concentration for colon carcinoma in rats took place in the interval between 1 to 4 h after PS injection.http://dx.doi.org/10.1155/2012/615259 |
| spellingShingle | Raduan Hage Juliana Ferreira Vanderlei Salvador Bagnato José Dirceu Vollet-Filho Hélio Plapler Pharmacokinetics of Photogem Using Fluorescence Spectroscopy in Dimethylhydrazine-Induced Murine Colorectal Carcinoma International Journal of Photoenergy |
| title | Pharmacokinetics of Photogem Using Fluorescence Spectroscopy in Dimethylhydrazine-Induced Murine Colorectal Carcinoma |
| title_full | Pharmacokinetics of Photogem Using Fluorescence Spectroscopy in Dimethylhydrazine-Induced Murine Colorectal Carcinoma |
| title_fullStr | Pharmacokinetics of Photogem Using Fluorescence Spectroscopy in Dimethylhydrazine-Induced Murine Colorectal Carcinoma |
| title_full_unstemmed | Pharmacokinetics of Photogem Using Fluorescence Spectroscopy in Dimethylhydrazine-Induced Murine Colorectal Carcinoma |
| title_short | Pharmacokinetics of Photogem Using Fluorescence Spectroscopy in Dimethylhydrazine-Induced Murine Colorectal Carcinoma |
| title_sort | pharmacokinetics of photogem using fluorescence spectroscopy in dimethylhydrazine induced murine colorectal carcinoma |
| url | http://dx.doi.org/10.1155/2012/615259 |
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