An in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines
Medicinal mushroom extracts, i.e. their dried biomass, have long been known as sources of bioactive compounds with positive effects on the human health. The antioxidant, antigenotoxic, antiviral, and immunomodulatory properties of the commercially available extracts Agaricus blazei auct. non Murrill...
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| Format: | Article |
| Language: | English |
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2024-12-01
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| Series: | Arhiv za Higijenu Rada i Toksikologiju |
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| Online Access: | https://doi.org/10.2478/aiht-2024-75-3915 |
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| author | Topalović Dijana Živković Lada Borozan Sunčica Santibanez Juan F. Spremo-Potparević Biljana |
| author_facet | Topalović Dijana Živković Lada Borozan Sunčica Santibanez Juan F. Spremo-Potparević Biljana |
| author_sort | Topalović Dijana |
| collection | DOAJ |
| description | Medicinal mushroom extracts, i.e. their dried biomass, have long been known as sources of bioactive compounds with positive effects on the human health. The antioxidant, antigenotoxic, antiviral, and immunomodulatory properties of the commercially available extracts Agaricus blazei auct. non Murrill (AB), Cordyceps sinensis (Berk.) Sacc. (CS), and Immune Assist (IA) have already been documented. This study, studied the influence of these three mushrooms on the viability of cell lines MCF-7, MDA-MB-231, and HS-5. The cytotoxicity of AB, CS, and IA at different concentrations (25, 50, 100, 200, 400 and 800 μg/mL) was evaluated using the MTT assay. The results showed that AB was the most effective and induced cytotoxicity in both cancer cell lines, with IC50 values of 96.7 μg/mL for MCF-7 and 368.4 μg/mL for MDA-MB-231. After treatment with CS and IA, the half-maximal inhibitory concentration was reached only in MDA- MB-231 cells (IC50=613 μg/mL for CS and 343.3 μg/mL for IA). We have shown here that AB, CS and IA can suppress the growth of MCF-7 and MDA-MB-231 cell lines, while affecting the survival of healthy HS-5 cells to a much lesser extent. Our in vitro results suggested that AB, CS and IA are promising natural sources with potential anticancer activity. |
| format | Article |
| id | doaj-art-fe5399bccedc4c9fbbdd5f4e9152e80a |
| institution | OA Journals |
| issn | 1848-6312 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Arhiv za Higijenu Rada i Toksikologiju |
| spelling | doaj-art-fe5399bccedc4c9fbbdd5f4e9152e80a2025-08-20T02:00:51ZengSciendoArhiv za Higijenu Rada i Toksikologiju1848-63122024-12-0175429730210.2478/aiht-2024-75-3915An in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell linesTopalović Dijana0Živković Lada1Borozan Sunčica2Santibanez Juan F.3Spremo-Potparević Biljana41University of Belgrade Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia1University of Belgrade Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia2University of Belgrade Faculty of Veterinary Medicine, Department of Chemistry, Belgrade, Serbia3University of Belgrade Institute for Medical Research National Institute of the Republic of Serbia, Department of Molecular Oncology, Belgrade, Serbia1University of Belgrade Faculty of Pharmacy, Department of Pathobiology, Belgrade, SerbiaMedicinal mushroom extracts, i.e. their dried biomass, have long been known as sources of bioactive compounds with positive effects on the human health. The antioxidant, antigenotoxic, antiviral, and immunomodulatory properties of the commercially available extracts Agaricus blazei auct. non Murrill (AB), Cordyceps sinensis (Berk.) Sacc. (CS), and Immune Assist (IA) have already been documented. This study, studied the influence of these three mushrooms on the viability of cell lines MCF-7, MDA-MB-231, and HS-5. The cytotoxicity of AB, CS, and IA at different concentrations (25, 50, 100, 200, 400 and 800 μg/mL) was evaluated using the MTT assay. The results showed that AB was the most effective and induced cytotoxicity in both cancer cell lines, with IC50 values of 96.7 μg/mL for MCF-7 and 368.4 μg/mL for MDA-MB-231. After treatment with CS and IA, the half-maximal inhibitory concentration was reached only in MDA- MB-231 cells (IC50=613 μg/mL for CS and 343.3 μg/mL for IA). We have shown here that AB, CS and IA can suppress the growth of MCF-7 and MDA-MB-231 cell lines, while affecting the survival of healthy HS-5 cells to a much lesser extent. Our in vitro results suggested that AB, CS and IA are promising natural sources with potential anticancer activity.https://doi.org/10.2478/aiht-2024-75-3915cytotoxicityagaricus blazeicordyceps sinensisimmune assistmttcitotoksičnostagaricus blazeicordyceps sinensisimmune assistmtt |
| spellingShingle | Topalović Dijana Živković Lada Borozan Sunčica Santibanez Juan F. Spremo-Potparević Biljana An in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines Arhiv za Higijenu Rada i Toksikologiju cytotoxicity agaricus blazei cordyceps sinensis immune assist mtt citotoksičnost agaricus blazei cordyceps sinensis immune assist mtt |
| title | An in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines |
| title_full | An in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines |
| title_fullStr | An in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines |
| title_full_unstemmed | An in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines |
| title_short | An in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines |
| title_sort | in vitro evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines |
| topic | cytotoxicity agaricus blazei cordyceps sinensis immune assist mtt citotoksičnost agaricus blazei cordyceps sinensis immune assist mtt |
| url | https://doi.org/10.2478/aiht-2024-75-3915 |
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