MEK inhibitors for the treatment of immunotherapy-resistant, AGK-BRAF fusion advanced acral melanoma: a case report and literature review

Abstract Purpose Acral melanoma (AM), a rare and aggressive melanoma subtype with poor prognosis, presents unique challenges in treatment due to its distinct molecular and immune characteristics. This case report describes a patient with AM harboring an AGK-BRAF fusion mutation, aiming to explore po...

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Main Authors: Yanling Zhang, Xifeng Zhang, Weikang Shao, Ji Gao, Mei Xiang, Yan Wang, Mengmeng Liu, Weizhen Zhang, Xianbin Liang
Format: Article
Language:English
Published: Springer 2025-04-01
Series:Journal of Cancer Research and Clinical Oncology
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Online Access:https://doi.org/10.1007/s00432-025-06083-3
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author Yanling Zhang
Xifeng Zhang
Weikang Shao
Ji Gao
Mei Xiang
Yan Wang
Mengmeng Liu
Weizhen Zhang
Xianbin Liang
author_facet Yanling Zhang
Xifeng Zhang
Weikang Shao
Ji Gao
Mei Xiang
Yan Wang
Mengmeng Liu
Weizhen Zhang
Xianbin Liang
author_sort Yanling Zhang
collection DOAJ
description Abstract Purpose Acral melanoma (AM), a rare and aggressive melanoma subtype with poor prognosis, presents unique challenges in treatment due to its distinct molecular and immune characteristics. This case report describes a patient with AM harboring an AGK-BRAF fusion mutation, aiming to explore potential mechanisms of resistance to current treatment modalities. Methods We analyzed tumor tissue samples from the primary and metastatic lesions of the patient using next-generation sequencing (NGS) for genomic profiling and multiplex immunohistochemistry (mIHC) to assess the immune microenvironment. The patient underwent multiple lines of treatment, including immunotherapy, chemotherapy, and targeted therapy, with their clinical outcomes documented and evaluated. Results The AGK-BRAF fusion mutation and its reciprocal BRAF-AGK rearrangement were identified in both primary and metastatic tumors. Immune profiling revealed abundant CD8 + T cells, PD-L1 + cells, and CD68 + macrophages localized predominantly in the tumor interstitial region, potentially explaining the poor response to immunotherapy. Despite initial disease stabilization with trametinib and lenvatinib, rapid progression occurred, highlighting tumor heterogeneity and limited efficacy of combined therapies. Conclusion This case underscores the need for personalized approaches in treating AM, especially those with rare molecular alterations like AGK-BRAF fusion. Insights from genomic and immune profiling may inform future therapeutic strategies to overcome resistance and improve outcomes in this challenging melanoma subtype.
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spelling doaj-art-fe4dd15a25b245caaf6fe7ea6f988cf62025-08-20T03:53:57ZengSpringerJournal of Cancer Research and Clinical Oncology1432-13352025-04-0115141910.1007/s00432-025-06083-3MEK inhibitors for the treatment of immunotherapy-resistant, AGK-BRAF fusion advanced acral melanoma: a case report and literature reviewYanling Zhang0Xifeng Zhang1Weikang Shao2Ji Gao3Mei Xiang4Yan Wang5Mengmeng Liu6Weizhen Zhang7Xianbin Liang8Department of Medical Oncology, The Third People’s Hospital of ZhengzhouDepartment of Medical Oncology, The Third People’s Hospital of ZhengzhouGenecast Biotechnology Co., LtdDepartment of Orthopaedics, The Third People’s Hospital of ZhengzhouDepartment of Medical Oncology, The Third People’s Hospital of ZhengzhouDepartment of Medical Oncology, The Third People’s Hospital of ZhengzhouDepartment of Pathology, The Third People’s Hospital of ZhengzhouDepartment of Medical Oncology, The Third People’s Hospital of ZhengzhouDepartment of Medical Oncology, The Third People’s Hospital of ZhengzhouAbstract Purpose Acral melanoma (AM), a rare and aggressive melanoma subtype with poor prognosis, presents unique challenges in treatment due to its distinct molecular and immune characteristics. This case report describes a patient with AM harboring an AGK-BRAF fusion mutation, aiming to explore potential mechanisms of resistance to current treatment modalities. Methods We analyzed tumor tissue samples from the primary and metastatic lesions of the patient using next-generation sequencing (NGS) for genomic profiling and multiplex immunohistochemistry (mIHC) to assess the immune microenvironment. The patient underwent multiple lines of treatment, including immunotherapy, chemotherapy, and targeted therapy, with their clinical outcomes documented and evaluated. Results The AGK-BRAF fusion mutation and its reciprocal BRAF-AGK rearrangement were identified in both primary and metastatic tumors. Immune profiling revealed abundant CD8 + T cells, PD-L1 + cells, and CD68 + macrophages localized predominantly in the tumor interstitial region, potentially explaining the poor response to immunotherapy. Despite initial disease stabilization with trametinib and lenvatinib, rapid progression occurred, highlighting tumor heterogeneity and limited efficacy of combined therapies. Conclusion This case underscores the need for personalized approaches in treating AM, especially those with rare molecular alterations like AGK-BRAF fusion. Insights from genomic and immune profiling may inform future therapeutic strategies to overcome resistance and improve outcomes in this challenging melanoma subtype.https://doi.org/10.1007/s00432-025-06083-3Acral melanomaAGK-BRAF fusionImmunotherapyTumor immune microenvironmentMEK inhibitors
spellingShingle Yanling Zhang
Xifeng Zhang
Weikang Shao
Ji Gao
Mei Xiang
Yan Wang
Mengmeng Liu
Weizhen Zhang
Xianbin Liang
MEK inhibitors for the treatment of immunotherapy-resistant, AGK-BRAF fusion advanced acral melanoma: a case report and literature review
Journal of Cancer Research and Clinical Oncology
Acral melanoma
AGK-BRAF fusion
Immunotherapy
Tumor immune microenvironment
MEK inhibitors
title MEK inhibitors for the treatment of immunotherapy-resistant, AGK-BRAF fusion advanced acral melanoma: a case report and literature review
title_full MEK inhibitors for the treatment of immunotherapy-resistant, AGK-BRAF fusion advanced acral melanoma: a case report and literature review
title_fullStr MEK inhibitors for the treatment of immunotherapy-resistant, AGK-BRAF fusion advanced acral melanoma: a case report and literature review
title_full_unstemmed MEK inhibitors for the treatment of immunotherapy-resistant, AGK-BRAF fusion advanced acral melanoma: a case report and literature review
title_short MEK inhibitors for the treatment of immunotherapy-resistant, AGK-BRAF fusion advanced acral melanoma: a case report and literature review
title_sort mek inhibitors for the treatment of immunotherapy resistant agk braf fusion advanced acral melanoma a case report and literature review
topic Acral melanoma
AGK-BRAF fusion
Immunotherapy
Tumor immune microenvironment
MEK inhibitors
url https://doi.org/10.1007/s00432-025-06083-3
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