Bioequivalence Study of Two Oral Methocarbamol Formulations in Healthy Subjects Under Fasting Conditions: A Randomized, Open-Label, Crossover Clinical Trial

<b>Objective:</b> This study aimed to assess the bioequivalence of two oral methocarbamol formulations, as follows: the test (T) methocarbamol 1500 mg tablets and the reference (R) Robaxin<sup>®</sup> 500 mg tablets (3 tablets, total dose: 1500 mg) under fasting conditions, a...

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Main Authors: Ana Ascaso-del-Rio, Paola Camargo-Mamani, Inmaculada Gilaberte, Mónica Díez-Hochleitner, Leonor Laredo-Velasco, Teresa Iglesias-Hernangómez, María Rosario Salas-Butrón, Laura Galán Caballero, Iván Alejandro Díaz-Rengifo, Carla Pérez-Ingidua, Emilio Vargas-Castrillón, Antonio Portolés-Pérez
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Pharmaceuticals
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Online Access:https://www.mdpi.com/1424-8247/18/3/354
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author Ana Ascaso-del-Rio
Paola Camargo-Mamani
Inmaculada Gilaberte
Mónica Díez-Hochleitner
Leonor Laredo-Velasco
Teresa Iglesias-Hernangómez
María Rosario Salas-Butrón
Laura Galán Caballero
Iván Alejandro Díaz-Rengifo
Carla Pérez-Ingidua
Emilio Vargas-Castrillón
Antonio Portolés-Pérez
author_facet Ana Ascaso-del-Rio
Paola Camargo-Mamani
Inmaculada Gilaberte
Mónica Díez-Hochleitner
Leonor Laredo-Velasco
Teresa Iglesias-Hernangómez
María Rosario Salas-Butrón
Laura Galán Caballero
Iván Alejandro Díaz-Rengifo
Carla Pérez-Ingidua
Emilio Vargas-Castrillón
Antonio Portolés-Pérez
author_sort Ana Ascaso-del-Rio
collection DOAJ
description <b>Objective:</b> This study aimed to assess the bioequivalence of two oral methocarbamol formulations, as follows: the test (T) methocarbamol 1500 mg tablets and the reference (R) Robaxin<sup>®</sup> 500 mg tablets (3 tablets, total dose: 1500 mg) under fasting conditions, and compare their pharmacokinetic performance. <b>Methods:</b> This was a single-center, phase I, randomized, open-label (blinded for analytical determination), two-sequence, two-period, crossover, bioequivalence study. A total of 32 healthy volunteers were randomly assigned to receive the T-R or R-T administration sequence. Each volunteer received a single dose of each methocarbamol formulation (T or R) separated by a washout period of 7 days. To evaluate the pharmacokinetic profile, blood samples were collected at nineteen time points after dosing. <b>Results:</b> The arithmetic mean C<sub>max</sub> was 31.72 µg/mL for R and 32.39 µg/mL for T, and the arithmetic mean AUC<sub>0−t</sub> was 90.25 h × µg/mL and 89.72 h × µg/mL, respectively. All adverse events reported were mild for both formulations. The 90% confidence intervals for the corresponding logarithmically transformed geometric mean ratios of C<sub>max</sub> and AUC<sub>0−t</sub> fell within the acceptance interval of 80.00–125.00%, as their values were 91.67–112.47% for ln(C<sub>max</sub>) and 92.34–103.47% for ln(AUC<sub>0−t</sub>). <b>Conclusion:</b> Therefore, one tablet of methocarbamol 1500 mg was found to be bioequivalent to the Robaxin<sup>®</sup> 500 mg tablets (3 tablets), with comparable tolerability and safety profiles.
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spelling doaj-art-fe4d01233fb04efb96fecc81e48011bf2025-08-20T03:43:37ZengMDPI AGPharmaceuticals1424-82472025-03-0118335410.3390/ph18030354Bioequivalence Study of Two Oral Methocarbamol Formulations in Healthy Subjects Under Fasting Conditions: A Randomized, Open-Label, Crossover Clinical TrialAna Ascaso-del-Rio0Paola Camargo-Mamani1Inmaculada Gilaberte2Mónica Díez-Hochleitner3Leonor Laredo-Velasco4Teresa Iglesias-Hernangómez5María Rosario Salas-Butrón6Laura Galán Caballero7Iván Alejandro Díaz-Rengifo8Carla Pérez-Ingidua9Emilio Vargas-Castrillón10Antonio Portolés-Pérez11Clinical Pharmacology Department, Hospital Clínico San Carlos, C/Prof Martín Lagos s/n, 28040 Madrid, SpainClinical Research Department, Faes Farma, Av. Autonomía 10, 48940 Leioa, SpainClinical Research Department, Faes Farma, Av. Autonomía 10, 48940 Leioa, SpainClinical Research Department, Faes Farma, Av. Autonomía 10, 48940 Leioa, SpainClinical Pharmacology Department, Hospital Clínico San Carlos, C/Prof Martín Lagos s/n, 28040 Madrid, SpainClinical Pharmacology Department, Hospital Clínico San Carlos, C/Prof Martín Lagos s/n, 28040 Madrid, SpainClinical Pharmacology Department, Hospital Clínico San Carlos, C/Prof Martín Lagos s/n, 28040 Madrid, SpainClinical Pharmacology Department, Hospital Clínico San Carlos, C/Prof Martín Lagos s/n, 28040 Madrid, SpainInstituto de Investigación Sanitaria del Hospital Clínico San Carlos, C/Prof Martín Lagos s/n, 28040 Madrid, SpainClinical Pharmacology Department, Hospital Clínico San Carlos, C/Prof Martín Lagos s/n, 28040 Madrid, SpainClinical Pharmacology Department, Hospital Clínico San Carlos, C/Prof Martín Lagos s/n, 28040 Madrid, SpainClinical Pharmacology Department, Hospital Clínico San Carlos, C/Prof Martín Lagos s/n, 28040 Madrid, Spain<b>Objective:</b> This study aimed to assess the bioequivalence of two oral methocarbamol formulations, as follows: the test (T) methocarbamol 1500 mg tablets and the reference (R) Robaxin<sup>®</sup> 500 mg tablets (3 tablets, total dose: 1500 mg) under fasting conditions, and compare their pharmacokinetic performance. <b>Methods:</b> This was a single-center, phase I, randomized, open-label (blinded for analytical determination), two-sequence, two-period, crossover, bioequivalence study. A total of 32 healthy volunteers were randomly assigned to receive the T-R or R-T administration sequence. Each volunteer received a single dose of each methocarbamol formulation (T or R) separated by a washout period of 7 days. To evaluate the pharmacokinetic profile, blood samples were collected at nineteen time points after dosing. <b>Results:</b> The arithmetic mean C<sub>max</sub> was 31.72 µg/mL for R and 32.39 µg/mL for T, and the arithmetic mean AUC<sub>0−t</sub> was 90.25 h × µg/mL and 89.72 h × µg/mL, respectively. All adverse events reported were mild for both formulations. The 90% confidence intervals for the corresponding logarithmically transformed geometric mean ratios of C<sub>max</sub> and AUC<sub>0−t</sub> fell within the acceptance interval of 80.00–125.00%, as their values were 91.67–112.47% for ln(C<sub>max</sub>) and 92.34–103.47% for ln(AUC<sub>0−t</sub>). <b>Conclusion:</b> Therefore, one tablet of methocarbamol 1500 mg was found to be bioequivalent to the Robaxin<sup>®</sup> 500 mg tablets (3 tablets), with comparable tolerability and safety profiles.https://www.mdpi.com/1424-8247/18/3/354Robaxinmethocarbamolbioequivalencebioequivalence studymethocarbamol pharmacokineticsmethocarbamol bioequivalence
spellingShingle Ana Ascaso-del-Rio
Paola Camargo-Mamani
Inmaculada Gilaberte
Mónica Díez-Hochleitner
Leonor Laredo-Velasco
Teresa Iglesias-Hernangómez
María Rosario Salas-Butrón
Laura Galán Caballero
Iván Alejandro Díaz-Rengifo
Carla Pérez-Ingidua
Emilio Vargas-Castrillón
Antonio Portolés-Pérez
Bioequivalence Study of Two Oral Methocarbamol Formulations in Healthy Subjects Under Fasting Conditions: A Randomized, Open-Label, Crossover Clinical Trial
Pharmaceuticals
Robaxin
methocarbamol
bioequivalence
bioequivalence study
methocarbamol pharmacokinetics
methocarbamol bioequivalence
title Bioequivalence Study of Two Oral Methocarbamol Formulations in Healthy Subjects Under Fasting Conditions: A Randomized, Open-Label, Crossover Clinical Trial
title_full Bioequivalence Study of Two Oral Methocarbamol Formulations in Healthy Subjects Under Fasting Conditions: A Randomized, Open-Label, Crossover Clinical Trial
title_fullStr Bioequivalence Study of Two Oral Methocarbamol Formulations in Healthy Subjects Under Fasting Conditions: A Randomized, Open-Label, Crossover Clinical Trial
title_full_unstemmed Bioequivalence Study of Two Oral Methocarbamol Formulations in Healthy Subjects Under Fasting Conditions: A Randomized, Open-Label, Crossover Clinical Trial
title_short Bioequivalence Study of Two Oral Methocarbamol Formulations in Healthy Subjects Under Fasting Conditions: A Randomized, Open-Label, Crossover Clinical Trial
title_sort bioequivalence study of two oral methocarbamol formulations in healthy subjects under fasting conditions a randomized open label crossover clinical trial
topic Robaxin
methocarbamol
bioequivalence
bioequivalence study
methocarbamol pharmacokinetics
methocarbamol bioequivalence
url https://www.mdpi.com/1424-8247/18/3/354
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