Role of lipid peroxidation in pathogenesis of senile cataract
Background /Aim. Cataract is a structural, biochemical and optical change in the eye lens, which changes transmission and refraction of light rays reducing keenness and clarity of a figure on the retina. Its occurrence is highest in older people, over the age of 65 (45.9%), thus a certain degree of...
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| Format: | Article |
| Language: | English |
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2009-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2009/0042-84500905371K.pdf |
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| author | Kisić Bojana Mirić Dijana Žorić Lepša Dragojević Ilija Stolić Aleksandra |
| author_facet | Kisić Bojana Mirić Dijana Žorić Lepša Dragojević Ilija Stolić Aleksandra |
| author_sort | Kisić Bojana |
| collection | DOAJ |
| description | Background /Aim. Cataract is a structural, biochemical and optical change in the eye lens, which changes transmission and refraction of light rays reducing keenness and clarity of a figure on the retina. Its occurrence is highest in older people, over the age of 65 (45.9%), thus a certain degree of opacification exists practically in all people over the 70. Our research was directed to measuring of lipid peroxidation products in cataract lenses involved in early stages of cataractogenesis through oxidative stress and in the development of mature cataract. Methods. Clinical and biochemical research was carried out in 101 patients with cataract, 46 women and 55 men. The average age of the group was 72.47 (ґ = 7.98). According to the cataract maturity degree the patients were classified into two groups as follows: cataracta senilis incipiens (n = 41) and cataracta senilis matura (n = 60). Measuring of diene conjugates was carried out by spectrophotometer. Fluorescent lipid peroxidation products were measured by a spectrofluorophotometer, and malondialdehyde (MDA) concentration was measured by colorimeter as a product of a reaction with thiobarbituric acid (TBA). Result. Significantly higher diene conjugated concentration in lenses was measured in the patients with the diagnosis cataracta senilis incipiens (p < 0.001) as well as the intensity of fluorescent iminopropens (p < 0.001). Significantly higher MDA concentration in lens (p < 0.001) was measured in the patients with cataracta senilis matura. Conclusion. The lens structure changes caused by lipid peroxidation can, with other risk factors present, influence the occurrence and development of mature cataract. Some cataract types show different lipid peroxidation intensity with the most distinct changes in cataract which started as corticonuclear. |
| format | Article |
| id | doaj-art-fe4724ab0e6546f0bc684f05934c37b3 |
| institution | DOAJ |
| issn | 0042-8450 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-fe4724ab0e6546f0bc684f05934c37b32025-08-20T03:06:17ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502009-01-0166537137510.2298/VSP0905371KRole of lipid peroxidation in pathogenesis of senile cataractKisić BojanaMirić DijanaŽorić LepšaDragojević IlijaStolić AleksandraBackground /Aim. Cataract is a structural, biochemical and optical change in the eye lens, which changes transmission and refraction of light rays reducing keenness and clarity of a figure on the retina. Its occurrence is highest in older people, over the age of 65 (45.9%), thus a certain degree of opacification exists practically in all people over the 70. Our research was directed to measuring of lipid peroxidation products in cataract lenses involved in early stages of cataractogenesis through oxidative stress and in the development of mature cataract. Methods. Clinical and biochemical research was carried out in 101 patients with cataract, 46 women and 55 men. The average age of the group was 72.47 (ґ = 7.98). According to the cataract maturity degree the patients were classified into two groups as follows: cataracta senilis incipiens (n = 41) and cataracta senilis matura (n = 60). Measuring of diene conjugates was carried out by spectrophotometer. Fluorescent lipid peroxidation products were measured by a spectrofluorophotometer, and malondialdehyde (MDA) concentration was measured by colorimeter as a product of a reaction with thiobarbituric acid (TBA). Result. Significantly higher diene conjugated concentration in lenses was measured in the patients with the diagnosis cataracta senilis incipiens (p < 0.001) as well as the intensity of fluorescent iminopropens (p < 0.001). Significantly higher MDA concentration in lens (p < 0.001) was measured in the patients with cataracta senilis matura. Conclusion. The lens structure changes caused by lipid peroxidation can, with other risk factors present, influence the occurrence and development of mature cataract. Some cataract types show different lipid peroxidation intensity with the most distinct changes in cataract which started as corticonuclear.http://www.doiserbia.nb.rs/img/doi/0042-8450/2009/0042-84500905371K.pdfcataractlipid peroxidationmalondialdehyde |
| spellingShingle | Kisić Bojana Mirić Dijana Žorić Lepša Dragojević Ilija Stolić Aleksandra Role of lipid peroxidation in pathogenesis of senile cataract Vojnosanitetski Pregled cataract lipid peroxidation malondialdehyde |
| title | Role of lipid peroxidation in pathogenesis of senile cataract |
| title_full | Role of lipid peroxidation in pathogenesis of senile cataract |
| title_fullStr | Role of lipid peroxidation in pathogenesis of senile cataract |
| title_full_unstemmed | Role of lipid peroxidation in pathogenesis of senile cataract |
| title_short | Role of lipid peroxidation in pathogenesis of senile cataract |
| title_sort | role of lipid peroxidation in pathogenesis of senile cataract |
| topic | cataract lipid peroxidation malondialdehyde |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2009/0042-84500905371K.pdf |
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