Development and Application of a Multiplex Reverse Transcription–Droplet Digital PCR Assay for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus in Bivalve Shellfish

Development and Application of a Multiplex Reverse Transcription–Droplet Digital PCR Assay for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus in Bivalve Shellfish

Foodborne viruses are significant contributors to global food safety incidents, posing a serious burden on human health and food safety. In this study, a multiplex reverse transcription–droplet digital PCR (RT-ddPCR) assay based on the MS2 phage as a process control virus (PCV) was developed to achi...

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Bibliographic Details
Main Authors: Maolin Wei, Jinfeng Wang, Yan Wang, Libing Liu, Xiangdong Xu, Jianchang Wang
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Foods
Subjects:
MS2 phage
multiplex
reverse transcription–droplet digital PCR
hepatitis A virus
hepatitis E virus
bivalve shellfish
Online Access:https://www.mdpi.com/2304-8158/14/1/2
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