Potential Involvement of <i>Buchnera aphidicola</i> (Enterobacteriales, Enterobacteriaceae) in Biotype Differentiation of <i>Sitobion avenae</i> (Hemiptera: Aphididae)
<i>Buchnera aphidicola</i>, an obligate endosymbiont of most aphid species, can influence aphids’ host adaptability through amino acid metabolism, potentially mediating biotype differentiation. However, its role in the biotype differentiation of <i>Sitobion avenae</i> remains...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/15/12/980 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846104329157607424 |
|---|---|
| author | Yanyan Lan Jingpeng Li Shuo Zhang Qiuju Qin Deguang Liu Chen Luo Shipeng Han Da Wang Yunzhuan He |
| author_facet | Yanyan Lan Jingpeng Li Shuo Zhang Qiuju Qin Deguang Liu Chen Luo Shipeng Han Da Wang Yunzhuan He |
| author_sort | Yanyan Lan |
| collection | DOAJ |
| description | <i>Buchnera aphidicola</i>, an obligate endosymbiont of most aphid species, can influence aphids’ host adaptability through amino acid metabolism, potentially mediating biotype differentiation. However, its role in the biotype differentiation of <i>Sitobion avenae</i> remains unclear. To address this issue, six <i>S. avenae</i> biotypes were tested in this study. <i>Buchnera</i> abundance varied among biotypes fed on different wheat/barley varieties (i.e., Zhong 4 wumang, 186-TM12-34; Dulihuang, Zaoshu No.3, Xiyin No.2). The reduction in <i>Buchnera</i> abundance through antibiotic (rifampicin) treatment altered the virulence of five <i>S. avenae</i> biotypes. Based on transcriptome analysis, the differential expression of three genes (i.e., <i>LeuB</i>, <i>TrpE</i>, and <i>IlvD</i>) related to leucine, tryptophan, isoleucine, and valine metabolism was detected between different biotypes. Principal component analysis showed that leucine and tryptophan deficiencies most significantly impacted nymph development duration and aphid fecundity. Additionally, a neighbor-joining phylogenetic tree indicated the genetic differentiation of <i>Buchnera</i> among different biotypes. These results suggest <i>Buchnera</i>-mediated amino acid metabolism is correlated with biotype differentiation in <i>S. avenae</i>, although the precise mechanisms by which <i>Buchnera</i> influences this differentiation require further investigation. This study can offer a theoretical basis for the development of resistant crops, leading to the sustainable control of this aphid and reduced reliance on chemical insecticides. |
| format | Article |
| id | doaj-art-fe3a46c76a0a46bd9451d8d68d111de3 |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-fe3a46c76a0a46bd9451d8d68d111de32024-12-27T14:31:23ZengMDPI AGInsects2075-44502024-12-01151298010.3390/insects15120980Potential Involvement of <i>Buchnera aphidicola</i> (Enterobacteriales, Enterobacteriaceae) in Biotype Differentiation of <i>Sitobion avenae</i> (Hemiptera: Aphididae)Yanyan Lan0Jingpeng Li1Shuo Zhang2Qiuju Qin3Deguang Liu4Chen Luo5Shipeng Han6Da Wang7Yunzhuan He8College of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaCollege of Plant Protection, Northwest A&F University, Yangling 712100, ChinaCollege of Plant Protection, Northwest A&F University, Yangling 712100, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071001, ChinaCollege of Plant Protection, Hebei Agricultural University, Baoding 071001, China<i>Buchnera aphidicola</i>, an obligate endosymbiont of most aphid species, can influence aphids’ host adaptability through amino acid metabolism, potentially mediating biotype differentiation. However, its role in the biotype differentiation of <i>Sitobion avenae</i> remains unclear. To address this issue, six <i>S. avenae</i> biotypes were tested in this study. <i>Buchnera</i> abundance varied among biotypes fed on different wheat/barley varieties (i.e., Zhong 4 wumang, 186-TM12-34; Dulihuang, Zaoshu No.3, Xiyin No.2). The reduction in <i>Buchnera</i> abundance through antibiotic (rifampicin) treatment altered the virulence of five <i>S. avenae</i> biotypes. Based on transcriptome analysis, the differential expression of three genes (i.e., <i>LeuB</i>, <i>TrpE</i>, and <i>IlvD</i>) related to leucine, tryptophan, isoleucine, and valine metabolism was detected between different biotypes. Principal component analysis showed that leucine and tryptophan deficiencies most significantly impacted nymph development duration and aphid fecundity. Additionally, a neighbor-joining phylogenetic tree indicated the genetic differentiation of <i>Buchnera</i> among different biotypes. These results suggest <i>Buchnera</i>-mediated amino acid metabolism is correlated with biotype differentiation in <i>S. avenae</i>, although the precise mechanisms by which <i>Buchnera</i> influences this differentiation require further investigation. This study can offer a theoretical basis for the development of resistant crops, leading to the sustainable control of this aphid and reduced reliance on chemical insecticides.https://www.mdpi.com/2075-4450/15/12/980artificial dietsbiotype differentiation<i>Buchnera</i> abundancegenetic variationgrain aphidRNA-seq |
| spellingShingle | Yanyan Lan Jingpeng Li Shuo Zhang Qiuju Qin Deguang Liu Chen Luo Shipeng Han Da Wang Yunzhuan He Potential Involvement of <i>Buchnera aphidicola</i> (Enterobacteriales, Enterobacteriaceae) in Biotype Differentiation of <i>Sitobion avenae</i> (Hemiptera: Aphididae) Insects artificial diets biotype differentiation <i>Buchnera</i> abundance genetic variation grain aphid RNA-seq |
| title | Potential Involvement of <i>Buchnera aphidicola</i> (Enterobacteriales, Enterobacteriaceae) in Biotype Differentiation of <i>Sitobion avenae</i> (Hemiptera: Aphididae) |
| title_full | Potential Involvement of <i>Buchnera aphidicola</i> (Enterobacteriales, Enterobacteriaceae) in Biotype Differentiation of <i>Sitobion avenae</i> (Hemiptera: Aphididae) |
| title_fullStr | Potential Involvement of <i>Buchnera aphidicola</i> (Enterobacteriales, Enterobacteriaceae) in Biotype Differentiation of <i>Sitobion avenae</i> (Hemiptera: Aphididae) |
| title_full_unstemmed | Potential Involvement of <i>Buchnera aphidicola</i> (Enterobacteriales, Enterobacteriaceae) in Biotype Differentiation of <i>Sitobion avenae</i> (Hemiptera: Aphididae) |
| title_short | Potential Involvement of <i>Buchnera aphidicola</i> (Enterobacteriales, Enterobacteriaceae) in Biotype Differentiation of <i>Sitobion avenae</i> (Hemiptera: Aphididae) |
| title_sort | potential involvement of i buchnera aphidicola i enterobacteriales enterobacteriaceae in biotype differentiation of i sitobion avenae i hemiptera aphididae |
| topic | artificial diets biotype differentiation <i>Buchnera</i> abundance genetic variation grain aphid RNA-seq |
| url | https://www.mdpi.com/2075-4450/15/12/980 |
| work_keys_str_mv | AT yanyanlan potentialinvolvementofibuchneraaphidicolaienterobacterialesenterobacteriaceaeinbiotypedifferentiationofisitobionavenaeihemipteraaphididae AT jingpengli potentialinvolvementofibuchneraaphidicolaienterobacterialesenterobacteriaceaeinbiotypedifferentiationofisitobionavenaeihemipteraaphididae AT shuozhang potentialinvolvementofibuchneraaphidicolaienterobacterialesenterobacteriaceaeinbiotypedifferentiationofisitobionavenaeihemipteraaphididae AT qiujuqin potentialinvolvementofibuchneraaphidicolaienterobacterialesenterobacteriaceaeinbiotypedifferentiationofisitobionavenaeihemipteraaphididae AT deguangliu potentialinvolvementofibuchneraaphidicolaienterobacterialesenterobacteriaceaeinbiotypedifferentiationofisitobionavenaeihemipteraaphididae AT chenluo potentialinvolvementofibuchneraaphidicolaienterobacterialesenterobacteriaceaeinbiotypedifferentiationofisitobionavenaeihemipteraaphididae AT shipenghan potentialinvolvementofibuchneraaphidicolaienterobacterialesenterobacteriaceaeinbiotypedifferentiationofisitobionavenaeihemipteraaphididae AT dawang potentialinvolvementofibuchneraaphidicolaienterobacterialesenterobacteriaceaeinbiotypedifferentiationofisitobionavenaeihemipteraaphididae AT yunzhuanhe potentialinvolvementofibuchneraaphidicolaienterobacterialesenterobacteriaceaeinbiotypedifferentiationofisitobionavenaeihemipteraaphididae |