Oligodendrocyte precursor cell transplantation attenuates inflammation after ischemic stroke in mice
BackgroundDisruption of blood–brain barrier and neuroinflammation are critical pathological features in the acute phase of ischemic stroke. This study investigates whether oligodendrocyte precursor cell transplantation can downregulate inflammation to attenuate blood–brain barrier disruption followi...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fneur.2025.1583982/full |
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| author | Li-Ping Wang Li-Ping Wang Chang Liu Yuanyuan Ma Aijuan Yan Guo-Yuan Yang Xinkai Qu Wenshi Wei |
| author_facet | Li-Ping Wang Li-Ping Wang Chang Liu Yuanyuan Ma Aijuan Yan Guo-Yuan Yang Xinkai Qu Wenshi Wei |
| author_sort | Li-Ping Wang |
| collection | DOAJ |
| description | BackgroundDisruption of blood–brain barrier and neuroinflammation are critical pathological features in the acute phase of ischemic stroke. This study investigates whether oligodendrocyte precursor cell transplantation can downregulate inflammation to attenuate blood–brain barrier disruption following ischemic brain injury.MethodsAdult male Institute of Cancer Research mice (n = 60) underwent transient middle cerebral artery occlusion. Post ischemic assault, these mice received a stereotactic injection of oligodendrocyte precursor cells (6 × 105). Neurobehavioral outcomes, infarct volume, inflammatory cytokines, myeloperoxidase, and tight junction protein levels were measured following ischemia.ResultsOligodendrocyte precursor cell transplantation reduced infarct volume, alleviated anxiety and depression, and promoted neurological recovery after ischemic stroke. Compared to the control group, oligodendrocyte precursor cell treated mice exhibited reduced levels of inflammatory cytokines IL-1β, IL-6, and TNF-α, reduced neutrophil infiltration, and diminished loss of tight junction protein. Oligodendrocyte precursor cells alleviated inflammation by increasing β-catenin expression. The administration of β-catenin inhibitor blocked the beneficial effects of oligodendrocyte precursor cell transplantation on neuroinflammation and blood–brain barrier permeability.ConclusionThis study demonstrates that oligodendrocyte precursor cell transplantation attenuates neuroinflammation and protectes blood–brain barrier in the acute phase of ischemic stroke. Our findings indicate that oligodendrocyte precursor cell transplantation is a promising therapeutic approach for ischemic stroke. |
| format | Article |
| id | doaj-art-fe3887f54fa6403b98a456695b489cc6 |
| institution | Kabale University |
| issn | 1664-2295 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neurology |
| spelling | doaj-art-fe3887f54fa6403b98a456695b489cc62025-08-20T03:50:49ZengFrontiers Media S.A.Frontiers in Neurology1664-22952025-07-011610.3389/fneur.2025.15839821583982Oligodendrocyte precursor cell transplantation attenuates inflammation after ischemic stroke in miceLi-Ping Wang0Li-Ping Wang1Chang Liu2Yuanyuan Ma3Aijuan Yan4Guo-Yuan Yang5Xinkai Qu6Wenshi Wei7Department of Neurology, Huadong Hospital, Fudan University, Shanghai, ChinaDepartment of Geriatric Medicine, Huadong Hospital, Fudan University, Shanghai, ChinaSchool of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Neurology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Neurology, Huadong Hospital, Fudan University, Shanghai, ChinaSchool of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Geriatric Medicine, Huadong Hospital, Fudan University, Shanghai, ChinaDepartment of Neurology, Huadong Hospital, Fudan University, Shanghai, ChinaBackgroundDisruption of blood–brain barrier and neuroinflammation are critical pathological features in the acute phase of ischemic stroke. This study investigates whether oligodendrocyte precursor cell transplantation can downregulate inflammation to attenuate blood–brain barrier disruption following ischemic brain injury.MethodsAdult male Institute of Cancer Research mice (n = 60) underwent transient middle cerebral artery occlusion. Post ischemic assault, these mice received a stereotactic injection of oligodendrocyte precursor cells (6 × 105). Neurobehavioral outcomes, infarct volume, inflammatory cytokines, myeloperoxidase, and tight junction protein levels were measured following ischemia.ResultsOligodendrocyte precursor cell transplantation reduced infarct volume, alleviated anxiety and depression, and promoted neurological recovery after ischemic stroke. Compared to the control group, oligodendrocyte precursor cell treated mice exhibited reduced levels of inflammatory cytokines IL-1β, IL-6, and TNF-α, reduced neutrophil infiltration, and diminished loss of tight junction protein. Oligodendrocyte precursor cells alleviated inflammation by increasing β-catenin expression. The administration of β-catenin inhibitor blocked the beneficial effects of oligodendrocyte precursor cell transplantation on neuroinflammation and blood–brain barrier permeability.ConclusionThis study demonstrates that oligodendrocyte precursor cell transplantation attenuates neuroinflammation and protectes blood–brain barrier in the acute phase of ischemic stroke. Our findings indicate that oligodendrocyte precursor cell transplantation is a promising therapeutic approach for ischemic stroke.https://www.frontiersin.org/articles/10.3389/fneur.2025.1583982/fullblood–brain barrierinflammationischemic strokeoligodendrocyte precursor celltransplantation |
| spellingShingle | Li-Ping Wang Li-Ping Wang Chang Liu Yuanyuan Ma Aijuan Yan Guo-Yuan Yang Xinkai Qu Wenshi Wei Oligodendrocyte precursor cell transplantation attenuates inflammation after ischemic stroke in mice Frontiers in Neurology blood–brain barrier inflammation ischemic stroke oligodendrocyte precursor cell transplantation |
| title | Oligodendrocyte precursor cell transplantation attenuates inflammation after ischemic stroke in mice |
| title_full | Oligodendrocyte precursor cell transplantation attenuates inflammation after ischemic stroke in mice |
| title_fullStr | Oligodendrocyte precursor cell transplantation attenuates inflammation after ischemic stroke in mice |
| title_full_unstemmed | Oligodendrocyte precursor cell transplantation attenuates inflammation after ischemic stroke in mice |
| title_short | Oligodendrocyte precursor cell transplantation attenuates inflammation after ischemic stroke in mice |
| title_sort | oligodendrocyte precursor cell transplantation attenuates inflammation after ischemic stroke in mice |
| topic | blood–brain barrier inflammation ischemic stroke oligodendrocyte precursor cell transplantation |
| url | https://www.frontiersin.org/articles/10.3389/fneur.2025.1583982/full |
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