Commercial bioinoculants improve colonization but do not alter the arbuscular mycorrhizal fungal community of greenhouse-grown grapevine roots
Abstract Background Arbuscular mycorrhizal fungi (AMF) are beneficial root symbionts contributing to improved plant growth and development and resistance to abiotic and biotic stresses. Commercial bioinoculants containing AMF are widely considered as an alternative to agrochemicals in vineyards. How...
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BMC
2025-01-01
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| Series: | Environmental Microbiome |
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| Online Access: | https://doi.org/10.1186/s40793-025-00676-8 |
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| author | Mariam P. Berdeja Nicole K. Reynolds Teresa Pawlowska Justine E. Vanden Heuvel |
| author_facet | Mariam P. Berdeja Nicole K. Reynolds Teresa Pawlowska Justine E. Vanden Heuvel |
| author_sort | Mariam P. Berdeja |
| collection | DOAJ |
| description | Abstract Background Arbuscular mycorrhizal fungi (AMF) are beneficial root symbionts contributing to improved plant growth and development and resistance to abiotic and biotic stresses. Commercial bioinoculants containing AMF are widely considered as an alternative to agrochemicals in vineyards. However, their effects on grapevine plants grown in soil containing native communities of AMF are still poorly understood. In a greenhouse experiment, we evaluated the influence of five different bioinoculants on the composition of native AMF communities of young Cabernet Sauvignon vines grown in a non-sterile soil. Root colonization, leaf nitrogen concentration, plant biomass and root morphology were assessed, and AMF communities of inoculated and non-inoculated grapevine roots were profiled using high-throughput sequencing. Results Contrary to our predictions, no differences in the microbiome of plants exposed to native AMF communities versus commercial AMF bioinoculants + native AMF communities were detected in roots. However, inoculation induced positive changes in root traits as well as increased AMF colonization, plant biomass, and leaf nitrogen. Most of these desirable functional traits were positively correlated with the relative abundance of operational taxonomic units identified as Glomus, Rhizophagus and Claroideoglomus genera. Conclusion These results suggest synergistic interactions between commercial AMF bioinoculants and native AMF communities of roots to promote grapevine growth. Long-term studies with further genomics, metabolomics and physiological research are needed to provide a deeper understanding of the symbiotic interaction among grapevine roots, bioinoculants and natural AMF communities and their role to promote plant adaptation to current environmental concerns. |
| format | Article |
| id | doaj-art-fe297b90250b4204975c9dd60270143b |
| institution | Kabale University |
| issn | 2524-6372 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | Environmental Microbiome |
| spelling | doaj-art-fe297b90250b4204975c9dd60270143b2025-02-02T12:43:14ZengBMCEnvironmental Microbiome2524-63722025-01-0120111510.1186/s40793-025-00676-8Commercial bioinoculants improve colonization but do not alter the arbuscular mycorrhizal fungal community of greenhouse-grown grapevine rootsMariam P. Berdeja0Nicole K. Reynolds1Teresa Pawlowska2Justine E. Vanden Heuvel3Horticulture Section, School of Integrative Plant Science, Cornell UniversityPlant Pathology and Plant Microbe-Biology Section, School of Integrative Plant Science, Cornell UniversityPlant Pathology and Plant Microbe-Biology Section, School of Integrative Plant Science, Cornell UniversityHorticulture Section, School of Integrative Plant Science, Cornell UniversityAbstract Background Arbuscular mycorrhizal fungi (AMF) are beneficial root symbionts contributing to improved plant growth and development and resistance to abiotic and biotic stresses. Commercial bioinoculants containing AMF are widely considered as an alternative to agrochemicals in vineyards. However, their effects on grapevine plants grown in soil containing native communities of AMF are still poorly understood. In a greenhouse experiment, we evaluated the influence of five different bioinoculants on the composition of native AMF communities of young Cabernet Sauvignon vines grown in a non-sterile soil. Root colonization, leaf nitrogen concentration, plant biomass and root morphology were assessed, and AMF communities of inoculated and non-inoculated grapevine roots were profiled using high-throughput sequencing. Results Contrary to our predictions, no differences in the microbiome of plants exposed to native AMF communities versus commercial AMF bioinoculants + native AMF communities were detected in roots. However, inoculation induced positive changes in root traits as well as increased AMF colonization, plant biomass, and leaf nitrogen. Most of these desirable functional traits were positively correlated with the relative abundance of operational taxonomic units identified as Glomus, Rhizophagus and Claroideoglomus genera. Conclusion These results suggest synergistic interactions between commercial AMF bioinoculants and native AMF communities of roots to promote grapevine growth. Long-term studies with further genomics, metabolomics and physiological research are needed to provide a deeper understanding of the symbiotic interaction among grapevine roots, bioinoculants and natural AMF communities and their role to promote plant adaptation to current environmental concerns.https://doi.org/10.1186/s40793-025-00676-8Arbuscular mycorrhizal fungiMetabarcodingBioinoculantsRoot traitsBiomassGrapevine |
| spellingShingle | Mariam P. Berdeja Nicole K. Reynolds Teresa Pawlowska Justine E. Vanden Heuvel Commercial bioinoculants improve colonization but do not alter the arbuscular mycorrhizal fungal community of greenhouse-grown grapevine roots Environmental Microbiome Arbuscular mycorrhizal fungi Metabarcoding Bioinoculants Root traits Biomass Grapevine |
| title | Commercial bioinoculants improve colonization but do not alter the arbuscular mycorrhizal fungal community of greenhouse-grown grapevine roots |
| title_full | Commercial bioinoculants improve colonization but do not alter the arbuscular mycorrhizal fungal community of greenhouse-grown grapevine roots |
| title_fullStr | Commercial bioinoculants improve colonization but do not alter the arbuscular mycorrhizal fungal community of greenhouse-grown grapevine roots |
| title_full_unstemmed | Commercial bioinoculants improve colonization but do not alter the arbuscular mycorrhizal fungal community of greenhouse-grown grapevine roots |
| title_short | Commercial bioinoculants improve colonization but do not alter the arbuscular mycorrhizal fungal community of greenhouse-grown grapevine roots |
| title_sort | commercial bioinoculants improve colonization but do not alter the arbuscular mycorrhizal fungal community of greenhouse grown grapevine roots |
| topic | Arbuscular mycorrhizal fungi Metabarcoding Bioinoculants Root traits Biomass Grapevine |
| url | https://doi.org/10.1186/s40793-025-00676-8 |
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