Cleavage Reaction Lateral Flow Assays for Salivary Pepsin Measurement Using a Pepsin-Susceptible Peptide Substrate
In this study, we introduce a novel cleavage reaction lateral flow assay (LFA) based on pepsin activity against a pepsin-susceptible peptide (PSP) substrate to detect salivary pepsin. Two types of cleavage reaction LFAs, the within-tube and on-strip cleavage reactions, were prepared based on the PSP...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/12/11/241 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846153991493255168 |
|---|---|
| author | Sung-Woong Kang Young Ju Lee Jae-Chul Lee Young-Gyu Eun Gi-Ja Lee |
| author_facet | Sung-Woong Kang Young Ju Lee Jae-Chul Lee Young-Gyu Eun Gi-Ja Lee |
| author_sort | Sung-Woong Kang |
| collection | DOAJ |
| description | In this study, we introduce a novel cleavage reaction lateral flow assay (LFA) based on pepsin activity against a pepsin-susceptible peptide (PSP) substrate to detect salivary pepsin. Two types of cleavage reaction LFAs, the within-tube and on-strip cleavage reactions, were prepared based on the PSP and pepsin reaction location. In the within-tube cleavage reaction LFA, samples were treated in the microtube within a heating block for 30 min separately and subsequently developed with running buffer in the LFA. For the on-strip cleavage reaction, samples were treated on the reaction zone of the strip within the heating zone of the multifunctional strip cassette for 10 min. After developing the running buffer in the LFA, the assay image was obtained using a universal mobile reader with a multifunctional strip cassette. The within-tube cleavage reaction LFA showed high sensitivity (limit of detection [LOD] 1.9 ng/mL), good specificity, and high reproducibility. This assay exhibited better linearity in the log concentration range of pepsin (4–500 ng/mL) than a commercially available dipstick assay. The on-strip cleavage reaction LFA showed a similar sensitivity (LOD 1.4 ng/mL) to that of the within-tube reaction assay. Therefore, we expect these cleavage reaction LFAs using PSP to be utilized as simple and effective tools to detect salivary pepsin. |
| format | Article |
| id | doaj-art-fe2925877fb847efa66e2eb5b3e850d5 |
| institution | Kabale University |
| issn | 2227-9040 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj-art-fe2925877fb847efa66e2eb5b3e850d52024-11-26T17:57:15ZengMDPI AGChemosensors2227-90402024-11-01121124110.3390/chemosensors12110241Cleavage Reaction Lateral Flow Assays for Salivary Pepsin Measurement Using a Pepsin-Susceptible Peptide SubstrateSung-Woong Kang0Young Ju Lee1Jae-Chul Lee2Young-Gyu Eun3Gi-Ja Lee4Department of Medical Engineering, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaDepartment of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Republic of KoreaTextile Innovation R&D Department, Korea Institute of Industrial Technology, Ansan 15588, Republic of KoreaDepartment of Otolaryngology–Head and Neck Surgery, Kyung Hee University College of Medicine, Kyung Hee University Medical Center, Seoul 02447, Republic of KoreaDepartment of Medical Engineering, Graduate School, Kyung Hee University, Seoul 02447, Republic of KoreaIn this study, we introduce a novel cleavage reaction lateral flow assay (LFA) based on pepsin activity against a pepsin-susceptible peptide (PSP) substrate to detect salivary pepsin. Two types of cleavage reaction LFAs, the within-tube and on-strip cleavage reactions, were prepared based on the PSP and pepsin reaction location. In the within-tube cleavage reaction LFA, samples were treated in the microtube within a heating block for 30 min separately and subsequently developed with running buffer in the LFA. For the on-strip cleavage reaction, samples were treated on the reaction zone of the strip within the heating zone of the multifunctional strip cassette for 10 min. After developing the running buffer in the LFA, the assay image was obtained using a universal mobile reader with a multifunctional strip cassette. The within-tube cleavage reaction LFA showed high sensitivity (limit of detection [LOD] 1.9 ng/mL), good specificity, and high reproducibility. This assay exhibited better linearity in the log concentration range of pepsin (4–500 ng/mL) than a commercially available dipstick assay. The on-strip cleavage reaction LFA showed a similar sensitivity (LOD 1.4 ng/mL) to that of the within-tube reaction assay. Therefore, we expect these cleavage reaction LFAs using PSP to be utilized as simple and effective tools to detect salivary pepsin.https://www.mdpi.com/2227-9040/12/11/241pepsinsalivalateral flow assaycleavage reactionpepsin-susceptible peptide substratemultifunctional strip cassette |
| spellingShingle | Sung-Woong Kang Young Ju Lee Jae-Chul Lee Young-Gyu Eun Gi-Ja Lee Cleavage Reaction Lateral Flow Assays for Salivary Pepsin Measurement Using a Pepsin-Susceptible Peptide Substrate Chemosensors pepsin saliva lateral flow assay cleavage reaction pepsin-susceptible peptide substrate multifunctional strip cassette |
| title | Cleavage Reaction Lateral Flow Assays for Salivary Pepsin Measurement Using a Pepsin-Susceptible Peptide Substrate |
| title_full | Cleavage Reaction Lateral Flow Assays for Salivary Pepsin Measurement Using a Pepsin-Susceptible Peptide Substrate |
| title_fullStr | Cleavage Reaction Lateral Flow Assays for Salivary Pepsin Measurement Using a Pepsin-Susceptible Peptide Substrate |
| title_full_unstemmed | Cleavage Reaction Lateral Flow Assays for Salivary Pepsin Measurement Using a Pepsin-Susceptible Peptide Substrate |
| title_short | Cleavage Reaction Lateral Flow Assays for Salivary Pepsin Measurement Using a Pepsin-Susceptible Peptide Substrate |
| title_sort | cleavage reaction lateral flow assays for salivary pepsin measurement using a pepsin susceptible peptide substrate |
| topic | pepsin saliva lateral flow assay cleavage reaction pepsin-susceptible peptide substrate multifunctional strip cassette |
| url | https://www.mdpi.com/2227-9040/12/11/241 |
| work_keys_str_mv | AT sungwoongkang cleavagereactionlateralflowassaysforsalivarypepsinmeasurementusingapepsinsusceptiblepeptidesubstrate AT youngjulee cleavagereactionlateralflowassaysforsalivarypepsinmeasurementusingapepsinsusceptiblepeptidesubstrate AT jaechullee cleavagereactionlateralflowassaysforsalivarypepsinmeasurementusingapepsinsusceptiblepeptidesubstrate AT younggyueun cleavagereactionlateralflowassaysforsalivarypepsinmeasurementusingapepsinsusceptiblepeptidesubstrate AT gijalee cleavagereactionlateralflowassaysforsalivarypepsinmeasurementusingapepsinsusceptiblepeptidesubstrate |