Protocol for the isolation of mouse fibro/adipogenic progenitors using magnetic-activated cell sorting
Summary: Fibro/adipogenic progenitors (FAPs) are mesenchymal progenitors that support muscle stem cell activation and modulate the extracellular matrix to facilitate muscle regeneration. Here, we present a protocol for the isolation of FAPs from mouse skeletal muscle. We describe steps for performin...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725001479 |
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| author | Jinghui Gao Yaochao Zheng Aria Sikal Elijah Sterling Rachel Hankin Yao Yao |
| author_facet | Jinghui Gao Yaochao Zheng Aria Sikal Elijah Sterling Rachel Hankin Yao Yao |
| author_sort | Jinghui Gao |
| collection | DOAJ |
| description | Summary: Fibro/adipogenic progenitors (FAPs) are mesenchymal progenitors that support muscle stem cell activation and modulate the extracellular matrix to facilitate muscle regeneration. Here, we present a protocol for the isolation of FAPs from mouse skeletal muscle. We describe steps for performing enzymatic digestion, mechanical dissociation, and magnetic-activated cell sorting (MACS). This protocol enables the isolation of FAPs with high yield and purity, facilitating research on muscle disorders and advancing muscle regeneration strategies. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-fe262f4bd1b94a9aa9f5b6e499e8e246 |
| institution | DOAJ |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-fe262f4bd1b94a9aa9f5b6e499e8e2462025-08-20T03:06:05ZengElsevierSTAR Protocols2666-16672025-06-016210374110.1016/j.xpro.2025.103741Protocol for the isolation of mouse fibro/adipogenic progenitors using magnetic-activated cell sortingJinghui Gao0Yaochao Zheng1Aria Sikal2Elijah Sterling3Rachel Hankin4Yao Yao5Regenerative Bioscience Center, Department of Animal and Dairy Science, College of Agricultural and Environmental Science, University of Georgia, Athens, GA 30602, USARegenerative Bioscience Center, Department of Animal and Dairy Science, College of Agricultural and Environmental Science, University of Georgia, Athens, GA 30602, USARegenerative Bioscience Center, Department of Animal and Dairy Science, College of Agricultural and Environmental Science, University of Georgia, Athens, GA 30602, USARegenerative Bioscience Center, Department of Animal and Dairy Science, College of Agricultural and Environmental Science, University of Georgia, Athens, GA 30602, USARegenerative Bioscience Center, Department of Animal and Dairy Science, College of Agricultural and Environmental Science, University of Georgia, Athens, GA 30602, USARegenerative Bioscience Center, Department of Animal and Dairy Science, College of Agricultural and Environmental Science, University of Georgia, Athens, GA 30602, USA; Corresponding authorSummary: Fibro/adipogenic progenitors (FAPs) are mesenchymal progenitors that support muscle stem cell activation and modulate the extracellular matrix to facilitate muscle regeneration. Here, we present a protocol for the isolation of FAPs from mouse skeletal muscle. We describe steps for performing enzymatic digestion, mechanical dissociation, and magnetic-activated cell sorting (MACS). This protocol enables the isolation of FAPs with high yield and purity, facilitating research on muscle disorders and advancing muscle regeneration strategies. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725001479Cell BiologyDevelopmental biologyGeneticsMolecular Biology |
| spellingShingle | Jinghui Gao Yaochao Zheng Aria Sikal Elijah Sterling Rachel Hankin Yao Yao Protocol for the isolation of mouse fibro/adipogenic progenitors using magnetic-activated cell sorting STAR Protocols Cell Biology Developmental biology Genetics Molecular Biology |
| title | Protocol for the isolation of mouse fibro/adipogenic progenitors using magnetic-activated cell sorting |
| title_full | Protocol for the isolation of mouse fibro/adipogenic progenitors using magnetic-activated cell sorting |
| title_fullStr | Protocol for the isolation of mouse fibro/adipogenic progenitors using magnetic-activated cell sorting |
| title_full_unstemmed | Protocol for the isolation of mouse fibro/adipogenic progenitors using magnetic-activated cell sorting |
| title_short | Protocol for the isolation of mouse fibro/adipogenic progenitors using magnetic-activated cell sorting |
| title_sort | protocol for the isolation of mouse fibro adipogenic progenitors using magnetic activated cell sorting |
| topic | Cell Biology Developmental biology Genetics Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725001479 |
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