A sterilization method for human decellularized vaginal matrices
Abstract Vaginal reconstruction is necessary for various congenital and acquired conditions, including vaginal aplasia, trauma, tumors, and gender incongruency. Current surgical and non-surgical treatments often result in significant complications. Decellularized vaginal matrices (DVMs) from human t...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-82409-4 |
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| author | Jayson Sueters Leonie de Boer Freek Groenman Judith A. F. Huirne Theo H. Smit Sebastian A. J. Zaat |
| author_facet | Jayson Sueters Leonie de Boer Freek Groenman Judith A. F. Huirne Theo H. Smit Sebastian A. J. Zaat |
| author_sort | Jayson Sueters |
| collection | DOAJ |
| description | Abstract Vaginal reconstruction is necessary for various congenital and acquired conditions, including vaginal aplasia, trauma, tumors, and gender incongruency. Current surgical and non-surgical treatments often result in significant complications. Decellularized vaginal matrices (DVMs) from human tissue offer a promising alternative, but require effective sterilization to ensure safety and functionality. This study aimed to develop a sterilization method for decellularized human vaginal wall scaffolds. Based on our previously implemented decellularization technique with minor modifications, we designed and examined three sterilization methods consisting of (i) chemical decellularization, (ii) decellularization with additional peracetic acid/hydrogen peroxide (PAA/H2O2); (iii) decellularization with antibiotic and antimycotic (AAE) based treatment. Sterilization efficacy was evaluated through controlled contamination with common vaginal microbes and sterility testing subsequent to each sterilization method. The extracellular matrix (ECM) structure was assessed via histological staining. Decellularization alone reduced some added bacterial contaminants but did not achieve complete sterilization. PAA/H2O2-sterilization resulted in severe ECM damage, rendering it unsuitable. The AAE-treatment demonstrated effective sterilization without compromising the ECM structure. Combined decellularization and AAE-based treatment forms a viable sterilization method for human vaginal wall tissue, maintaining ECM integrity and achieving effective micro-organism elimination. This method holds potential for clinical application in vaginal transplantation. |
| format | Article |
| id | doaj-art-fe1dcf90556f496d914dcdc7adeee76b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-fe1dcf90556f496d914dcdc7adeee76b2025-01-05T12:28:08ZengNature PortfolioScientific Reports2045-23222024-12-0114111410.1038/s41598-024-82409-4A sterilization method for human decellularized vaginal matricesJayson Sueters0Leonie de Boer1Freek Groenman2Judith A. F. Huirne3Theo H. Smit4Sebastian A. J. Zaat5Department of Gynaecology, Amsterdam UMC – Location VUmcDepartment of Medical Microbiology and Infection Prevention, Amsterdam UMC – Location AMCDepartment of Obstetrics and Gynecology, Amsterdam Reproduction and Development, Amsterdam UMC – Location VUmcDepartment of Gynaecology, Amsterdam UMC – Location VUmcDepartment of Gynaecology, Amsterdam UMC – Location VUmcDepartment of Medical Microbiology and Infection Prevention, Amsterdam UMC – Location AMCAbstract Vaginal reconstruction is necessary for various congenital and acquired conditions, including vaginal aplasia, trauma, tumors, and gender incongruency. Current surgical and non-surgical treatments often result in significant complications. Decellularized vaginal matrices (DVMs) from human tissue offer a promising alternative, but require effective sterilization to ensure safety and functionality. This study aimed to develop a sterilization method for decellularized human vaginal wall scaffolds. Based on our previously implemented decellularization technique with minor modifications, we designed and examined three sterilization methods consisting of (i) chemical decellularization, (ii) decellularization with additional peracetic acid/hydrogen peroxide (PAA/H2O2); (iii) decellularization with antibiotic and antimycotic (AAE) based treatment. Sterilization efficacy was evaluated through controlled contamination with common vaginal microbes and sterility testing subsequent to each sterilization method. The extracellular matrix (ECM) structure was assessed via histological staining. Decellularization alone reduced some added bacterial contaminants but did not achieve complete sterilization. PAA/H2O2-sterilization resulted in severe ECM damage, rendering it unsuitable. The AAE-treatment demonstrated effective sterilization without compromising the ECM structure. Combined decellularization and AAE-based treatment forms a viable sterilization method for human vaginal wall tissue, maintaining ECM integrity and achieving effective micro-organism elimination. This method holds potential for clinical application in vaginal transplantation.https://doi.org/10.1038/s41598-024-82409-4DecellularizationSterilization methodVaginaHuman acellular matrix |
| spellingShingle | Jayson Sueters Leonie de Boer Freek Groenman Judith A. F. Huirne Theo H. Smit Sebastian A. J. Zaat A sterilization method for human decellularized vaginal matrices Scientific Reports Decellularization Sterilization method Vagina Human acellular matrix |
| title | A sterilization method for human decellularized vaginal matrices |
| title_full | A sterilization method for human decellularized vaginal matrices |
| title_fullStr | A sterilization method for human decellularized vaginal matrices |
| title_full_unstemmed | A sterilization method for human decellularized vaginal matrices |
| title_short | A sterilization method for human decellularized vaginal matrices |
| title_sort | sterilization method for human decellularized vaginal matrices |
| topic | Decellularization Sterilization method Vagina Human acellular matrix |
| url | https://doi.org/10.1038/s41598-024-82409-4 |
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